Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform
Background: Oropharyngeal (OP) and nasopharyngeal (NP) sampling has historically been considered the reference specimen type used for respiratory virus detection. Saliva could be a less invasive alternative for SARS-CoV-2 detection, but limited evidence is available. Methods: The technical and clini...
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MDPI AG
2023-01-01
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| Serija: | Diagnostics |
| Teme: | |
| Online pristup: | https://www.mdpi.com/2075-4418/13/3/347 |
| _version_ | 1827760287045910528 |
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| author | Ali Vahidnia Dennis Souverein Sjoerd M. Euser Milly Haverkort Elise Noordhuis Thomas Z. I. van Zijl Jayant Kalpoe Jan C. Sinnige Bjorn L. Herpers |
| author_facet | Ali Vahidnia Dennis Souverein Sjoerd M. Euser Milly Haverkort Elise Noordhuis Thomas Z. I. van Zijl Jayant Kalpoe Jan C. Sinnige Bjorn L. Herpers |
| author_sort | Ali Vahidnia |
| collection | DOAJ |
| description | Background: Oropharyngeal (OP) and nasopharyngeal (NP) sampling has historically been considered the reference specimen type used for respiratory virus detection. Saliva could be a less invasive alternative for SARS-CoV-2 detection, but limited evidence is available. Methods: The technical and clinical performance of saliva was compared to OP/NP on the Hologic Panther platform with two Aptima assays, the End-Point Transcription-Mediated Amplification assay (EP-TMA) and Real-Time Transcription-Mediated Amplification assay (RT-TMA). The samples were collected at the Public Health Service Testing Site XL location in Schiphol Amsterdam Airport. At the site, the Regional Public Health Laboratory Kennemerland (RPHLK) has a fully equipped laboratory facility. Results: A total of 374 samples (187 OP/NP swabs and 187 saliva samples) were collected from 187 unique patients. The Real-Time Transcription-Mediated Amplification assay (RT-TMA) resulted in comparable sensitivities for the detection of SARS-CoV-2 in both the OP/NP swabs (88.3%; 113/128) and saliva samples (87.5%; 112/128). The End-Point Transcription-Mediated Amplification assay (EP-TMA) analyses showed a similar sensitivity (86.7%; 111/128) in the OP/NP swabs but a lower sensitivity in the saliva samples (80.5%; 103/128). Within the discordant analyses, we found no associations in the symptoms, earlier SARS-CoV-2 infections and eating, smoking, drinking and tooth brushing habits within one hour before testing. Conclusions: The Hologic Panther platform Real-Time Transcription-Mediated Amplification assay (RT-TMA) yields a sensitivity for the detection of SARS-CoV-2 in saliva that is comparable to the OP/NP swabs derived from participants presenting themselves at a public health testing facility with minimal or mild symptoms. |
| first_indexed | 2024-03-11T09:49:27Z |
| format | Article |
| id | doaj.art-bdf9e7155b42403d9d58c2e02599db39 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T09:49:27Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-bdf9e7155b42403d9d58c2e02599db392023-11-16T16:23:25ZengMDPI AGDiagnostics2075-44182023-01-0113334710.3390/diagnostics13030347Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther PlatformAli Vahidnia0Dennis Souverein1Sjoerd M. Euser2Milly Haverkort3Elise Noordhuis4Thomas Z. I. van Zijl5Jayant Kalpoe6Jan C. Sinnige7Bjorn L. Herpers8Regional Public Health Laboratory Kennemerland, 2035 RC Haarlem, The NetherlandsRegional Public Health Laboratory Kennemerland, 2035 RC Haarlem, The NetherlandsRegional Public Health Laboratory Kennemerland, 2035 RC Haarlem, The NetherlandsPublic Health Service Kennemerland, 2015 CK Haarlem, The NetherlandsPublic Health Service Kennemerland, 2015 CK Haarlem, The NetherlandsPublic Health Service Kennemerland, 2015 CK Haarlem, The NetherlandsRegional Public Health Laboratory Kennemerland, 2035 RC Haarlem, The NetherlandsRegional Public Health Laboratory Kennemerland, 2035 RC Haarlem, The NetherlandsRegional Public Health Laboratory Kennemerland, 2035 RC Haarlem, The NetherlandsBackground: Oropharyngeal (OP) and nasopharyngeal (NP) sampling has historically been considered the reference specimen type used for respiratory virus detection. Saliva could be a less invasive alternative for SARS-CoV-2 detection, but limited evidence is available. Methods: The technical and clinical performance of saliva was compared to OP/NP on the Hologic Panther platform with two Aptima assays, the End-Point Transcription-Mediated Amplification assay (EP-TMA) and Real-Time Transcription-Mediated Amplification assay (RT-TMA). The samples were collected at the Public Health Service Testing Site XL location in Schiphol Amsterdam Airport. At the site, the Regional Public Health Laboratory Kennemerland (RPHLK) has a fully equipped laboratory facility. Results: A total of 374 samples (187 OP/NP swabs and 187 saliva samples) were collected from 187 unique patients. The Real-Time Transcription-Mediated Amplification assay (RT-TMA) resulted in comparable sensitivities for the detection of SARS-CoV-2 in both the OP/NP swabs (88.3%; 113/128) and saliva samples (87.5%; 112/128). The End-Point Transcription-Mediated Amplification assay (EP-TMA) analyses showed a similar sensitivity (86.7%; 111/128) in the OP/NP swabs but a lower sensitivity in the saliva samples (80.5%; 103/128). Within the discordant analyses, we found no associations in the symptoms, earlier SARS-CoV-2 infections and eating, smoking, drinking and tooth brushing habits within one hour before testing. Conclusions: The Hologic Panther platform Real-Time Transcription-Mediated Amplification assay (RT-TMA) yields a sensitivity for the detection of SARS-CoV-2 in saliva that is comparable to the OP/NP swabs derived from participants presenting themselves at a public health testing facility with minimal or mild symptoms.https://www.mdpi.com/2075-4418/13/3/347salivaTMASARS-CoV-2Hologicsensitivity |
| spellingShingle | Ali Vahidnia Dennis Souverein Sjoerd M. Euser Milly Haverkort Elise Noordhuis Thomas Z. I. van Zijl Jayant Kalpoe Jan C. Sinnige Bjorn L. Herpers Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform Diagnostics saliva TMA SARS-CoV-2 Hologic sensitivity |
| title | Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform |
| title_full | Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform |
| title_fullStr | Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform |
| title_full_unstemmed | Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform |
| title_short | Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform |
| title_sort | similar sensitivity of sars cov 2 detection in oropharyngeal nasopharyngeal and saliva samples on the hologic panther platform |
| topic | saliva TMA SARS-CoV-2 Hologic sensitivity |
| url | https://www.mdpi.com/2075-4418/13/3/347 |
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