Point-of-Care Testing – Biosensor for Norepinephrine Determination
An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of l...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2020-06-01
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| Series: | International Journal of Electronics and Telecommunications |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/115214/PDF/50_2020.pdf |
| _version_ | 1811343580320497664 |
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| author | Sylwia Baluta Agnieszka Swist Joanna Cabaj Karol Malecha |
| author_facet | Sylwia Baluta Agnieszka Swist Joanna Cabaj Karol Malecha |
| author_sort | Sylwia Baluta |
| collection | DOAJ |
| description | An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with thin polymeric film. This sensing arrangement utilized the catalytic oxidation of NE to norepinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of enzyme – laccase. With the optimized conditions, the analytical performance demonstrated selectivity in a wide linear range (0.1–200x10-6 M) with a detection limit of 240 nM and a quantification limit of 365 nM. Moreover, the method was successfully applied for selective NE determination in the presence of interfering substances. |
| first_indexed | 2024-04-13T19:32:38Z |
| format | Article |
| id | doaj.art-bdf9ecdc252f44b8aed1adccd056bb7a |
| institution | Directory Open Access Journal |
| issn | 2081-8491 2300-1933 |
| language | English |
| last_indexed | 2024-04-13T19:32:38Z |
| publishDate | 2020-06-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | International Journal of Electronics and Telecommunications |
| spelling | doaj.art-bdf9ecdc252f44b8aed1adccd056bb7a2022-12-22T02:33:09ZengPolish Academy of SciencesInternational Journal of Electronics and Telecommunications2081-84912300-19332020-06-01vol. 66No 2369372https://doi.org/10.24425/ijet.2020.131887Point-of-Care Testing – Biosensor for Norepinephrine DeterminationSylwia BalutaAgnieszka SwistJoanna CabajKarol MalechaAn useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with thin polymeric film. This sensing arrangement utilized the catalytic oxidation of NE to norepinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of enzyme – laccase. With the optimized conditions, the analytical performance demonstrated selectivity in a wide linear range (0.1–200x10-6 M) with a detection limit of 240 nM and a quantification limit of 365 nM. Moreover, the method was successfully applied for selective NE determination in the presence of interfering substances.https://journals.pan.pl/Content/115214/PDF/50_2020.pdfbiosensorcyclic voltammetrylaccasenorepinephrinepoint-of-care |
| spellingShingle | Sylwia Baluta Agnieszka Swist Joanna Cabaj Karol Malecha Point-of-Care Testing – Biosensor for Norepinephrine Determination International Journal of Electronics and Telecommunications biosensor cyclic voltammetry laccase norepinephrine point-of-care |
| title | Point-of-Care Testing – Biosensor for Norepinephrine Determination |
| title_full | Point-of-Care Testing – Biosensor for Norepinephrine Determination |
| title_fullStr | Point-of-Care Testing – Biosensor for Norepinephrine Determination |
| title_full_unstemmed | Point-of-Care Testing – Biosensor for Norepinephrine Determination |
| title_short | Point-of-Care Testing – Biosensor for Norepinephrine Determination |
| title_sort | point of care testing biosensor for norepinephrine determination |
| topic | biosensor cyclic voltammetry laccase norepinephrine point-of-care |
| url | https://journals.pan.pl/Content/115214/PDF/50_2020.pdf |
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