Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp.
Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 a...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2003-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/163960 |
| _version_ | 1797423652193435648 |
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| author | Andreas Paar Alexander Raninger Fernanda de Sousa Ivo Beurer Artur Cavaco-Paulo Georg M. Gübitz |
| author_facet | Andreas Paar Alexander Raninger Fernanda de Sousa Ivo Beurer Artur Cavaco-Paulo Georg M. Gübitz |
| author_sort | Andreas Paar |
| collection | DOAJ |
| description | Catalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment. |
| first_indexed | 2024-03-09T07:50:00Z |
| format | Article |
| id | doaj.art-bdfc877912b44ca98b9de1fd2f6972d0 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:50:00Z |
| publishDate | 2003-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-bdfc877912b44ca98b9de1fd2f6972d02023-12-03T02:07:04ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062003-01-01412101104Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp.Andreas Paar0Alexander Raninger1Fernanda de Sousa2Ivo Beurer3Artur Cavaco-Paulo4Georg M. Gübitz5Graz University of Technology, Department of Environmental Biotechnology, Petersgasse 12, A-8010 Graz, AustriaGraz University of Technology, Department of Environmental Biotechnology, Petersgasse 12, A-8010 Graz, AustriaUniversity of Minho, Textile Engineering Department, P-4800 Guimaraes, PortugalEcole Polytechnique Fédérale de Lausanne, SwitzerlandUniversity of Minho, Textile Engineering Department, P-4800 Guimaraes, PortugalGraz University of Technology, Department of Environmental Biotechnology, Petersgasse 12, A-8010 Graz, AustriaCatalase-peroxidase (CP) production by a Bacillus sp. (Bacillus KF) newly isolated from a textile finishing effluent and by this strain immobilised on light expanded clay was studied. In cultivations of Bacillus KF increased catalase activity (about 30-fold) was measured after the addition of H2O2 and Orange IV, while ascorbic acid, pyrogallol and Paraquat, seemed to be poor inducers. Catalases in the cell extract from Bacillus KF showed remarkable stability at high temperatures and pH values with half-lifes of 20 h at pH=9 and 60 °C while half-lifes based on the activity of catalases of only 2.2 h were measured in a column reactor during hydrogen peroxide degradation for the whole cells. However, after the addition of cultivation medium immobilised cells can be regenerated and thus used for textile bleaching effluent treatment.http://hrcak.srce.hr/file/163960catalase-peroxidaseBacillus sp.bleaching |
| spellingShingle | Andreas Paar Alexander Raninger Fernanda de Sousa Ivo Beurer Artur Cavaco-Paulo Georg M. Gübitz Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp. Food Technology and Biotechnology catalase-peroxidase Bacillus sp. bleaching |
| title | Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp. |
| title_full | Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp. |
| title_fullStr | Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp. |
| title_full_unstemmed | Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp. |
| title_short | Production of Catalase-Peroxidase and Continuous Degradation of Hydrogen Peroxide by an Immobilised Alkalothermophilic Bacillus sp. |
| title_sort | production of catalase peroxidase and continuous degradation of hydrogen peroxide by an immobilised alkalothermophilic bacillus sp |
| topic | catalase-peroxidase Bacillus sp. bleaching |
| url | http://hrcak.srce.hr/file/163960 |
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