Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i>
Sleep–wake stability is imbalanced with natural aging, and microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and aging; however, the biological functions of miRNAs in regulating aging-related sleep–wake behavior remain unexplored. This study varied the expression pattern of d...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/4/4180 |
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| author | Qiufang Li Lingxiao Wang Yurou Cao Xiaoya Wang Chao Tang Lan Zheng |
| author_facet | Qiufang Li Lingxiao Wang Yurou Cao Xiaoya Wang Chao Tang Lan Zheng |
| author_sort | Qiufang Li |
| collection | DOAJ |
| description | Sleep–wake stability is imbalanced with natural aging, and microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and aging; however, the biological functions of miRNAs in regulating aging-related sleep–wake behavior remain unexplored. This study varied the expression pattern of dmiR-283 in <i>Drosophila</i> and the result showed that the aging decline in sleep–wake behavior was caused by the accumulation of brain dmiR-283 expression, whereas the core clock genes <i>cwo</i> and Notch signaling pathway might be suppressed, which regulate the aging process. In addition, to identify exercise intervention programs of <i>Drosophila</i> that promote healthy aging, mir-283<sup>SP</sup>/+ and <i>Pdf</i> > mir-283<sup>SP</sup> flies were driven to perform endurance exercise for a duration of 3 weeks starting at 10 and 30 days, respectively. The results showed that exercise starting in youth leads to an enhanced amplitude of sleep–wake rhythms, stable periods, increased activity frequency upon awakening, and the suppression of aging brain dmiR-283 expression in mir-283<sup>SP</sup>/+ middle-aged flies. Conversely, exercise performed when the brain dmiR-283 reached a certain accumulation level showed ineffective or negative effects. In conclusion, the accumulation of dmiR-283 expression in the brain induced an age-dependent decline in sleep–wake behavior. Endurance exercise commencing in youth counteracts the increase in dmiR-283 in the aging brain, which ameliorates the deterioration of sleep–wake behavior during aging. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:41:12Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bdfd1b4ee4c44ffca65048bfd48fabf12023-11-16T21:10:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244418010.3390/ijms24044180Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i>Qiufang Li0Lingxiao Wang1Yurou Cao2Xiaoya Wang3Chao Tang4Lan Zheng5Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha 410012, ChinaThe Center for Heart Development, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha 410081, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha 410012, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha 410012, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha 410012, ChinaKey Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University, Changsha 410012, ChinaSleep–wake stability is imbalanced with natural aging, and microRNAs (miRNAs) play important roles in cell proliferation, apoptosis, and aging; however, the biological functions of miRNAs in regulating aging-related sleep–wake behavior remain unexplored. This study varied the expression pattern of dmiR-283 in <i>Drosophila</i> and the result showed that the aging decline in sleep–wake behavior was caused by the accumulation of brain dmiR-283 expression, whereas the core clock genes <i>cwo</i> and Notch signaling pathway might be suppressed, which regulate the aging process. In addition, to identify exercise intervention programs of <i>Drosophila</i> that promote healthy aging, mir-283<sup>SP</sup>/+ and <i>Pdf</i> > mir-283<sup>SP</sup> flies were driven to perform endurance exercise for a duration of 3 weeks starting at 10 and 30 days, respectively. The results showed that exercise starting in youth leads to an enhanced amplitude of sleep–wake rhythms, stable periods, increased activity frequency upon awakening, and the suppression of aging brain dmiR-283 expression in mir-283<sup>SP</sup>/+ middle-aged flies. Conversely, exercise performed when the brain dmiR-283 reached a certain accumulation level showed ineffective or negative effects. In conclusion, the accumulation of dmiR-283 expression in the brain induced an age-dependent decline in sleep–wake behavior. Endurance exercise commencing in youth counteracts the increase in dmiR-283 in the aging brain, which ameliorates the deterioration of sleep–wake behavior during aging.https://www.mdpi.com/1422-0067/24/4/4180miRNAagingsleep–wake behaviorcircadian rhythmexercise |
| spellingShingle | Qiufang Li Lingxiao Wang Yurou Cao Xiaoya Wang Chao Tang Lan Zheng Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i> International Journal of Molecular Sciences miRNA aging sleep–wake behavior circadian rhythm exercise |
| title | Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i> |
| title_full | Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i> |
| title_fullStr | Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i> |
| title_full_unstemmed | Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i> |
| title_short | Stable Expression of dmiR-283 in the Brain Promises Positive Effects in Endurance Exercise on Sleep–Wake Behavior in Aging <i>Drosophila</i> |
| title_sort | stable expression of dmir 283 in the brain promises positive effects in endurance exercise on sleep wake behavior in aging i drosophila i |
| topic | miRNA aging sleep–wake behavior circadian rhythm exercise |
| url | https://www.mdpi.com/1422-0067/24/4/4180 |
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