Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017
Background and Objectives: Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in many populations, including military recruits receiving basic training. Therefore, this study was set out to determine the molecular epidemiology, genotype and phylogenetic featu...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-10-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2692 |
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| author | Mohammad Hadi Karbalaie Niya Ali Salimi Jeda Ahmad Tavakoli Hesam Sohani Fahimeh Safarnezhad Tameshkel Mahshid Panahi Morteza Izadi Farhad Zamani Hossein Keyvani |
| author_facet | Mohammad Hadi Karbalaie Niya Ali Salimi Jeda Ahmad Tavakoli Hesam Sohani Fahimeh Safarnezhad Tameshkel Mahshid Panahi Morteza Izadi Farhad Zamani Hossein Keyvani |
| author_sort | Mohammad Hadi Karbalaie Niya |
| collection | DOAJ |
| description | Background and Objectives: Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in many populations, including military recruits receiving basic training. Therefore, this study was set out to determine the molecular epidemiology, genotype and phylogenetic features of RSVs in patients with respiratory infection as a case study.
Materials and Methods: In this study, military barracks of Tehran, Iran, between January to March 2017 exposed to respiratory diseases were used for sampling. Throat swabs were taken, a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was performed to identify RSV and then the genotyping and phylogenetic analyses of RSVs in patients with a respiratory infection.
Results: Among 400 Iranian military trainees with respiratory symptoms, RSV infection was identified in 2.75% (11/400) using RT-PCR. Sequencing showed the incidence of type A (2.5%, n=10) to be much higher than type B (0.25%, n=1); Sore throat was the most common symptom among RSV patients. Phylogenetic analysis revealed that the majority of strains from the studied samples were more consistent with those from the Philippines and the US strains.
Conclusion: This study is the first to document RSV as a major cause of acute respiratory illness among military trainees in Iran. The prevalence of RSV is substantial in the cold season and the prevalence of genotype A is dominant in the country, leading to take essential steps in preparing a preventive vaccine against this viral infection. |
| first_indexed | 2024-12-20T17:30:05Z |
| format | Article |
| id | doaj.art-be02b6169d354010b4ddb54cf28405d9 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-20T17:30:05Z |
| publishDate | 2020-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-be02b6169d354010b4ddb54cf28405d92022-12-21T19:31:26ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472020-10-0112510.18502/ijm.v12i5.4612Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017Mohammad Hadi Karbalaie Niya0Ali Salimi Jeda1Ahmad Tavakoli2Hesam Sohani3Fahimeh Safarnezhad Tameshkel4Mahshid Panahi5Morteza Izadi6Farhad Zamani7Hossein Keyvani8Gastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran AND Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran AND Pediatric Infectious Diseases Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Faculty of Biology, College of Sciences, University of Sciences and Research, Tehran, IranGastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, IranGastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, IranHealth Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranGastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, IranGastrointestinal and Liver Diseases Research Center, Iran University of Medical Sciences, Tehran, Iran AND Department of Medical Virology, School of Medicine, Iran University of Medical Sciences, Tehran, IranBackground and Objectives: Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in many populations, including military recruits receiving basic training. Therefore, this study was set out to determine the molecular epidemiology, genotype and phylogenetic features of RSVs in patients with respiratory infection as a case study. Materials and Methods: In this study, military barracks of Tehran, Iran, between January to March 2017 exposed to respiratory diseases were used for sampling. Throat swabs were taken, a reverse transcriptase-polymerase chain reaction (RT-PCR) assay was performed to identify RSV and then the genotyping and phylogenetic analyses of RSVs in patients with a respiratory infection. Results: Among 400 Iranian military trainees with respiratory symptoms, RSV infection was identified in 2.75% (11/400) using RT-PCR. Sequencing showed the incidence of type A (2.5%, n=10) to be much higher than type B (0.25%, n=1); Sore throat was the most common symptom among RSV patients. Phylogenetic analysis revealed that the majority of strains from the studied samples were more consistent with those from the Philippines and the US strains. Conclusion: This study is the first to document RSV as a major cause of acute respiratory illness among military trainees in Iran. The prevalence of RSV is substantial in the cold season and the prevalence of genotype A is dominant in the country, leading to take essential steps in preparing a preventive vaccine against this viral infection.https://ijm.tums.ac.ir/index.php/ijm/article/view/2692Respiratory tract infection;Human respiratory syncytial virus;Military trainees;Reverse transcription polymerase chain reaction;Genotyping |
| spellingShingle | Mohammad Hadi Karbalaie Niya Ali Salimi Jeda Ahmad Tavakoli Hesam Sohani Fahimeh Safarnezhad Tameshkel Mahshid Panahi Morteza Izadi Farhad Zamani Hossein Keyvani Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017 Iranian Journal of Microbiology Respiratory tract infection; Human respiratory syncytial virus; Military trainees; Reverse transcription polymerase chain reaction; Genotyping |
| title | Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017 |
| title_full | Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017 |
| title_fullStr | Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017 |
| title_full_unstemmed | Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017 |
| title_short | Molecular epidemiology of human respiratory syncytial virus (HRSV) in Iranian military trainees with acute respiratory symptoms in 2017 |
| title_sort | molecular epidemiology of human respiratory syncytial virus hrsv in iranian military trainees with acute respiratory symptoms in 2017 |
| topic | Respiratory tract infection; Human respiratory syncytial virus; Military trainees; Reverse transcription polymerase chain reaction; Genotyping |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/2692 |
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