Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i>
Plasmid-mediated quinolone resistance (PMQR) remains one of the main mechanisms of bacterial quinolone resistance and plays an important role in the transmission of antibiotic resistance genes (ARGs). In this study, two novel plasmids, p3M-2A and p3M-2B, which mediate quinolone resistance in <i&g...
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MDPI AG
2020-07-01
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| author | Hongyang Zhang Mingding Chang Xiaochen Zhang Peiyan Cai Yixin Dai Tongzhen Song Zhenzhou Wu Haijin Xu Mingqiang Qiao |
| author_facet | Hongyang Zhang Mingding Chang Xiaochen Zhang Peiyan Cai Yixin Dai Tongzhen Song Zhenzhou Wu Haijin Xu Mingqiang Qiao |
| author_sort | Hongyang Zhang |
| collection | DOAJ |
| description | Plasmid-mediated quinolone resistance (PMQR) remains one of the main mechanisms of bacterial quinolone resistance and plays an important role in the transmission of antibiotic resistance genes (ARGs). In this study, two novel plasmids, p3M-2A and p3M-2B, which mediate quinolone resistance in <i>Proteus vulgaris</i> strain 3M (P3M) were identified. Of these, only p3M-2B appeared to be a <i>qnrD</i>-carrying plasmid. Both p3M-2A and p3M-2B could be transferred into <i>Escherichia coli</i>, and the latter caused a twofold change in ciprofloxacin resistance, according to the measured minimum inhibitory concentration (MIC). Plasmid curing/complementation and qRT-PCR results showed that p3M-2A can directly regulate the expression of <i>qnrD</i> in p3M-2B under treatment with ciprofloxacin, in which process, <i>ORF1</i> was found to play an important role. Sequence alignments and phylogenetic analysis revealed the evolutionary relationships of all reported <i>qnrD</i>-carrying plasmids and showed that <i>ORF1–4</i> in p3M-2B is the most conserved backbone for the normal function of <i>qnrD</i>-carrying plasmids. The identified direct repeats (DR) suggested that, from an evolutionary perspective, p3M-2B may have originated from the 2683-bp <i>qnrD</i>-carrying plasmid and may increase the possibility of plasmid recombination and then of <i>qnrD</i> transfer. To the best of our knowledge, this is the first identification of a novel <i>qnrD</i>-carrying plasmid isolated from a <i>P. vulgaris</i> strain of shrimp origin and a plasmid that plays a regulatory role in <i>qnrD</i> expression. This study also sheds new light on plasmid evolution and on the mechanism of horizontal transfer of ARGs encoded by plasmids. |
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| spelling | doaj.art-be066949bb9d4883b34e4503f37558472023-11-20T07:12:30ZengMDPI AGMicroorganisms2076-26072020-07-0187107410.3390/microorganisms8071074Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i>Hongyang Zhang0Mingding Chang1Xiaochen Zhang2Peiyan Cai3Yixin Dai4Tongzhen Song5Zhenzhou Wu6Haijin Xu7Mingqiang Qiao8The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaZhengzhou University Industrial Technology Institute Co. Ltd., Zhengzhou 450000, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, ChinaPlasmid-mediated quinolone resistance (PMQR) remains one of the main mechanisms of bacterial quinolone resistance and plays an important role in the transmission of antibiotic resistance genes (ARGs). In this study, two novel plasmids, p3M-2A and p3M-2B, which mediate quinolone resistance in <i>Proteus vulgaris</i> strain 3M (P3M) were identified. Of these, only p3M-2B appeared to be a <i>qnrD</i>-carrying plasmid. Both p3M-2A and p3M-2B could be transferred into <i>Escherichia coli</i>, and the latter caused a twofold change in ciprofloxacin resistance, according to the measured minimum inhibitory concentration (MIC). Plasmid curing/complementation and qRT-PCR results showed that p3M-2A can directly regulate the expression of <i>qnrD</i> in p3M-2B under treatment with ciprofloxacin, in which process, <i>ORF1</i> was found to play an important role. Sequence alignments and phylogenetic analysis revealed the evolutionary relationships of all reported <i>qnrD</i>-carrying plasmids and showed that <i>ORF1–4</i> in p3M-2B is the most conserved backbone for the normal function of <i>qnrD</i>-carrying plasmids. The identified direct repeats (DR) suggested that, from an evolutionary perspective, p3M-2B may have originated from the 2683-bp <i>qnrD</i>-carrying plasmid and may increase the possibility of plasmid recombination and then of <i>qnrD</i> transfer. To the best of our knowledge, this is the first identification of a novel <i>qnrD</i>-carrying plasmid isolated from a <i>P. vulgaris</i> strain of shrimp origin and a plasmid that plays a regulatory role in <i>qnrD</i> expression. This study also sheds new light on plasmid evolution and on the mechanism of horizontal transfer of ARGs encoded by plasmids.https://www.mdpi.com/2076-2607/8/7/1074quinolone resistance<i>Proteus vulgaris</i><i>qnrD</i>-carrying plasmids<i>qnrD</i> expressiondirect repeatshomologous recombination |
| spellingShingle | Hongyang Zhang Mingding Chang Xiaochen Zhang Peiyan Cai Yixin Dai Tongzhen Song Zhenzhou Wu Haijin Xu Mingqiang Qiao Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i> Microorganisms quinolone resistance <i>Proteus vulgaris</i> <i>qnrD</i>-carrying plasmids <i>qnrD</i> expression direct repeats homologous recombination |
| title | Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i> |
| title_full | Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i> |
| title_fullStr | Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i> |
| title_full_unstemmed | Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i> |
| title_short | Functional Identification and Evolutionary Analysis of Two Novel Plasmids Mediating Quinolone Resistance in <i>Proteus vulgaris</i> |
| title_sort | functional identification and evolutionary analysis of two novel plasmids mediating quinolone resistance in i proteus vulgaris i |
| topic | quinolone resistance <i>Proteus vulgaris</i> <i>qnrD</i>-carrying plasmids <i>qnrD</i> expression direct repeats homologous recombination |
| url | https://www.mdpi.com/2076-2607/8/7/1074 |
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