Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
IntroductionIndustrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomin...
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| Format: | Article |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1092184/full |
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| author | Pablo Martínez-Rodríguez Iván Sánchez-Castro Jesús J. Ojeda María M. Abad Michael Descostes Michael Descostes Mohamed Larbi Merroun |
| author_facet | Pablo Martínez-Rodríguez Iván Sánchez-Castro Jesús J. Ojeda María M. Abad Michael Descostes Michael Descostes Mohamed Larbi Merroun |
| author_sort | Pablo Martínez-Rodríguez |
| collection | DOAJ |
| description | IntroductionIndustrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g., phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unraveled to optimize results when applying this remediation process.MethodsIn this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.).Results and DiscussionIt was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48 h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. These findings show the biotechnological use of this strain in the bioremediation of U as a function of phosphate substrate: U biomineralization (in a phosphate free system) and indirectly through the solubilization of orthophosphate from phosphate (P) containing waste products needed for U precipitation. |
| first_indexed | 2024-04-11T00:04:45Z |
| format | Article |
| id | doaj.art-be09c7c5783c4aafafca6f3c2918c66a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T00:04:45Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-be09c7c5783c4aafafca6f3c2918c66a2023-01-09T13:07:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10921841092184Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach studyPablo Martínez-Rodríguez0Iván Sánchez-Castro1Jesús J. Ojeda2María M. Abad3Michael Descostes4Michael Descostes5Mohamed Larbi Merroun6Department of Microbiology, University of Granada, Granada, SpainDepartment of Microbiology, University of Granada, Granada, SpainDepartment of Chemical Engineering, Faculty of Science and Engineering, Swansea University, Swansea, United KingdomCentro de Instrumentación Científica (CIC), University of Granada, Granada, SpainEnvironmental R&D Department, ORANO Mining, Chatillon, FranceCentre de Géosciences, MINES Paris, PSL University, Fontainebleau, FranceDepartment of Microbiology, University of Granada, Granada, SpainIntroductionIndustrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g., phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unraveled to optimize results when applying this remediation process.MethodsIn this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.).Results and DiscussionIt was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48 h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. These findings show the biotechnological use of this strain in the bioremediation of U as a function of phosphate substrate: U biomineralization (in a phosphate free system) and indirectly through the solubilization of orthophosphate from phosphate (P) containing waste products needed for U precipitation.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1092184/fullMicrobacteriumuraniumphosphate sourcebioprecipitationsolubilizationPSB |
| spellingShingle | Pablo Martínez-Rodríguez Iván Sánchez-Castro Jesús J. Ojeda María M. Abad Michael Descostes Michael Descostes Mohamed Larbi Merroun Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study Frontiers in Microbiology Microbacterium uranium phosphate source bioprecipitation solubilization PSB |
| title | Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study |
| title_full | Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study |
| title_fullStr | Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study |
| title_full_unstemmed | Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study |
| title_short | Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study |
| title_sort | effect of different phosphate sources on uranium biomineralization by the microbacterium sp be9 strain a multidisciplinary approach study |
| topic | Microbacterium uranium phosphate source bioprecipitation solubilization PSB |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1092184/full |
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