Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice
Introduction: Hydatid Disease (HD), a helminth infection with various clinical complications is a serious economic and public health concern around the world. Nowadays, different agents have been broadly applied as scolicids; these agents have several side effects on patients. Therefore, the aims of...
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| Format: | Article |
| Language: | English |
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Ilam University of Medical Sciences
2018-01-01
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| Series: | Journal of Basic Research in Medical Sciences |
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| Online Access: | http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-127-7&slc_lang=en&sid=1 |
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| author | Arash Babaei Arash Jafari Mohammad Asadpour Morteza Shamsi |
| author_facet | Arash Babaei Arash Jafari Mohammad Asadpour Morteza Shamsi |
| author_sort | Arash Babaei |
| collection | DOAJ |
| description | Introduction: Hydatid Disease (HD), a helminth infection with various clinical complications is a serious economic and public health concern around the world. Nowadays, different agents have been broadly applied as scolicids; these agents have several side effects on patients. Therefore, the aims of this experimental study were to evaluate the scolicidal activity of Cucurbita maxima seeds methanolic extract (ME) against protoscolices of hydatid cyst in-vitro. Also, an in vivo assay was designed to prove the preventive effects of the extract.
Materials and methods: For this work, Two different concentrations (25 and 50 mg/ml) of the C. maxima seeds ME were used in various exposure times (10, 20, 30, 40, 50, and 60 min). Additionally, thirty mice were infected intraperitoneally by injection of 1000 viable protoscolices and were divided into three groups with ten mice in each Group I (treated by Albendazole), group II (received 50 mg/ml of C. maxima seeds ME) and group III (untreated controls).
Results: Overall, C. maxima seeds ME exhibited significant scolicidal effects in compared with control group (P<0.05). At a concentration of 50 mg/ml, the mortality percentage increased to 84.7%, 96.1% and 100% after 40, 50 and 60 minutes, respectively. Also, the obtain data in necropsy demonstrated that C. maxima seeds ME have an inhibitory effect on the hydatid cyst development. No hydatid cyst formation observed in mice treated with C. maxima seeds ME (50 mg/ml) versus control group which hydatid cyst occurred in 6 (from 10) mice.
Conclusion: The current investigation indicated that applying C. maxima seeds ME could be considered as a potential scolicidal agent due to being economical, safer and non-toxic compared to the reference chemical drugs. However, further studies on probably active compounds and also to the determination of its mode of action on parasites are required. |
| first_indexed | 2024-12-10T14:28:35Z |
| format | Article |
| id | doaj.art-be0a0247a50d4329b6d99f0af339053c |
| institution | Directory Open Access Journal |
| issn | 2383-0506 2383-0972 |
| language | English |
| last_indexed | 2024-12-10T14:28:35Z |
| publishDate | 2018-01-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Journal of Basic Research in Medical Sciences |
| spelling | doaj.art-be0a0247a50d4329b6d99f0af339053c2022-12-22T01:45:00ZengIlam University of Medical SciencesJournal of Basic Research in Medical Sciences2383-05062383-09722018-01-01512228Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in miceArash Babaei0Arash Jafari1Mohammad Asadpour2Morteza Shamsi3 Department of Biology, Faculty of Sciences, Malayer University, Malayer, Iran Department of Pathobiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran Department of Pathobiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran Department of Parasitology, Faculty of Paramedical Sciences, Ilam University of Medical Sciences, Ilam, Iran Introduction: Hydatid Disease (HD), a helminth infection with various clinical complications is a serious economic and public health concern around the world. Nowadays, different agents have been broadly applied as scolicids; these agents have several side effects on patients. Therefore, the aims of this experimental study were to evaluate the scolicidal activity of Cucurbita maxima seeds methanolic extract (ME) against protoscolices of hydatid cyst in-vitro. Also, an in vivo assay was designed to prove the preventive effects of the extract. Materials and methods: For this work, Two different concentrations (25 and 50 mg/ml) of the C. maxima seeds ME were used in various exposure times (10, 20, 30, 40, 50, and 60 min). Additionally, thirty mice were infected intraperitoneally by injection of 1000 viable protoscolices and were divided into three groups with ten mice in each Group I (treated by Albendazole), group II (received 50 mg/ml of C. maxima seeds ME) and group III (untreated controls). Results: Overall, C. maxima seeds ME exhibited significant scolicidal effects in compared with control group (P<0.05). At a concentration of 50 mg/ml, the mortality percentage increased to 84.7%, 96.1% and 100% after 40, 50 and 60 minutes, respectively. Also, the obtain data in necropsy demonstrated that C. maxima seeds ME have an inhibitory effect on the hydatid cyst development. No hydatid cyst formation observed in mice treated with C. maxima seeds ME (50 mg/ml) versus control group which hydatid cyst occurred in 6 (from 10) mice. Conclusion: The current investigation indicated that applying C. maxima seeds ME could be considered as a potential scolicidal agent due to being economical, safer and non-toxic compared to the reference chemical drugs. However, further studies on probably active compounds and also to the determination of its mode of action on parasites are required.http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-127-7&slc_lang=en&sid=1Hydatid diseaseCucurbita maxima methanolic extractsScolicidalPreventive assay |
| spellingShingle | Arash Babaei Arash Jafari Mohammad Asadpour Morteza Shamsi Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice Journal of Basic Research in Medical Sciences Hydatid disease Cucurbita maxima methanolic extracts Scolicidal Preventive assay |
| title | Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice |
| title_full | Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice |
| title_fullStr | Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice |
| title_full_unstemmed | Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice |
| title_short | Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice |
| title_sort | cucurbita maxima pumpkin seeds scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice |
| topic | Hydatid disease Cucurbita maxima methanolic extracts Scolicidal Preventive assay |
| url | http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-127-7&slc_lang=en&sid=1 |
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