Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration

Background: The regeneration of lost/damaged support tissue in the periodontium, including the alveolar bone, periodontal ligament, and cementum, is an ambitious purpose of periodontal regenerative therapy and might effectively reduce periodontitis-caused tooth loss. Guided tissue regeneration (GTR)...

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Main Authors: Akansha Kishen, Anju Cecil, S Chitra
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:Saudi Dental Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1013905223001037
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author Akansha Kishen
Anju Cecil
S Chitra
author_facet Akansha Kishen
Anju Cecil
S Chitra
author_sort Akansha Kishen
collection DOAJ
description Background: The regeneration of lost/damaged support tissue in the periodontium, including the alveolar bone, periodontal ligament, and cementum, is an ambitious purpose of periodontal regenerative therapy and might effectively reduce periodontitis-caused tooth loss. Guided tissue regeneration (GTR) is a technique currently used in dentistry for periodontal surgery, which allows osseous regeneration prior to soft tissue migration into the area of interest. Calcium phosphate-based bone grafts (mostly Tricalcium Phosphate or Hydroxyapatite) are bio ceramics that show the greatest similarity to the mineral found in the bone. Thereby, giving calcium-phosphate excellent biocompatibility, biodegradability and osteoconductivity. The aim of the study is to fabricate hydroxyapatite reinforced polymeric hydrogel membrane for regeneration. Materials and Method: Pure alginate fabrication was done by cross linking sodium alginate with calcium chloride. Hydroxyapatite (HAP) alginate (Alg) was formulated by adding nanoparticles to the alginate mixture, which was then cross-linked with calcium chloride to formulate a HAP alginate polymeric membrane. The Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), and biocompatibility tests were performed to analyse the membrane characteristics. Results: Fabricated Hydroxyapatite- alginate (Hap- Alg) membrane has longer durability, because of strong crystal structure which in turn might take a longer time to regenerate. The membrane was found to be biocompatible and HAp induces faster mineralisation which in turn will increase the tissue regeneration rate of the membrane. Conclusion: The findings of our study suggests that the HAP-Alg hydro gel membrane is highly durable and hemocompatible and it has faster mineralisation capability thus making it superior from the clinically available membranes for GTR. Further analyses needs to be conducted to evaluate the potential of this membrane to be used for regeneration.
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spelling doaj.art-be0c2d7ca138417a92d5ad03cd480c392023-09-24T05:14:18ZengElsevierSaudi Dental Journal1013-90522023-09-01356678683Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regenerationAkansha Kishen0Anju Cecil1S Chitra2Saveetha Dental College and hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai-600077, Tamil Nadu, IndiaDepartment of Periodontics, Saveetha Dental College and hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai-600077, Tamil Nadu, India; Corresponding author at: Department of Periodontics, Saveetha Dental College and hospitals Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai-600077, Tamil Nadu, India.Department of Biomaterials, Saveetha Dental College and hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha University, Chennai-600077, Tamil Nadu, IndiaBackground: The regeneration of lost/damaged support tissue in the periodontium, including the alveolar bone, periodontal ligament, and cementum, is an ambitious purpose of periodontal regenerative therapy and might effectively reduce periodontitis-caused tooth loss. Guided tissue regeneration (GTR) is a technique currently used in dentistry for periodontal surgery, which allows osseous regeneration prior to soft tissue migration into the area of interest. Calcium phosphate-based bone grafts (mostly Tricalcium Phosphate or Hydroxyapatite) are bio ceramics that show the greatest similarity to the mineral found in the bone. Thereby, giving calcium-phosphate excellent biocompatibility, biodegradability and osteoconductivity. The aim of the study is to fabricate hydroxyapatite reinforced polymeric hydrogel membrane for regeneration. Materials and Method: Pure alginate fabrication was done by cross linking sodium alginate with calcium chloride. Hydroxyapatite (HAP) alginate (Alg) was formulated by adding nanoparticles to the alginate mixture, which was then cross-linked with calcium chloride to formulate a HAP alginate polymeric membrane. The Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), and biocompatibility tests were performed to analyse the membrane characteristics. Results: Fabricated Hydroxyapatite- alginate (Hap- Alg) membrane has longer durability, because of strong crystal structure which in turn might take a longer time to regenerate. The membrane was found to be biocompatible and HAp induces faster mineralisation which in turn will increase the tissue regeneration rate of the membrane. Conclusion: The findings of our study suggests that the HAP-Alg hydro gel membrane is highly durable and hemocompatible and it has faster mineralisation capability thus making it superior from the clinically available membranes for GTR. Further analyses needs to be conducted to evaluate the potential of this membrane to be used for regeneration.http://www.sciencedirect.com/science/article/pii/S1013905223001037HAP membraneHydro gelGTRPeriodontal therapyDentistryHydroxyapatite
spellingShingle Akansha Kishen
Anju Cecil
S Chitra
Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
Saudi Dental Journal
HAP membrane
Hydro gel
GTR
Periodontal therapy
Dentistry
Hydroxyapatite
title Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
title_full Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
title_fullStr Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
title_full_unstemmed Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
title_short Fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
title_sort fabrication of hydroxyapatite reinforced polymeric hydrogel membrane for regeneration
topic HAP membrane
Hydro gel
GTR
Periodontal therapy
Dentistry
Hydroxyapatite
url http://www.sciencedirect.com/science/article/pii/S1013905223001037
work_keys_str_mv AT akanshakishen fabricationofhydroxyapatitereinforcedpolymerichydrogelmembraneforregeneration
AT anjucecil fabricationofhydroxyapatitereinforcedpolymerichydrogelmembraneforregeneration
AT schitra fabricationofhydroxyapatitereinforcedpolymerichydrogelmembraneforregeneration