In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh
Seaweed belongs to marine biota and contains nutrients and secondary metabolites beneficial for health. This study aimed to determine the antidiabetic activity of extracts and fractions of green seaweed <i>Halimeda tuna</i>. The <i>H. tuna</i> sample was extracted with the ma...
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/2/393 |
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| author | Mohamad Gazali Odi Jolanda Amir Husni Nurjanah Fadzilah Adibah Abd Majid Zuriat Rina Syafitri |
| author_facet | Mohamad Gazali Odi Jolanda Amir Husni Nurjanah Fadzilah Adibah Abd Majid Zuriat Rina Syafitri |
| author_sort | Mohamad Gazali |
| collection | DOAJ |
| description | Seaweed belongs to marine biota and contains nutrients and secondary metabolites beneficial for health. This study aimed to determine the antidiabetic activity of extracts and fractions of green seaweed <i>Halimeda tuna</i>. The <i>H. tuna</i> sample was extracted with the maceration method using methanol and then partitioned using ethyl acetate and water to obtain ethyl acetate and water fractions. The methanol extract, ethyl acetate fraction, and water fraction of <i>H. tuna</i> were tested for their inhibitory activity against α-amilase and α-glucosidase. The methanol extract and the fractions with the highest inhibitory activity were phytochemically tested and analyzed using gas chromatography–mass spectrometry (GC-MS). The results showed that the ethyl acetate fraction (IC<sub>50</sub> = 0.88 ± 0.20 mg/mL) inhibited α-amylase relatively similar to acarbose (IC<sub>50</sub> = 0.76 ± 0.04 mg/mL). The methanol extract (IC<sub>50</sub> = 0.05 ± 0.01 mg/mL) and the ethyl acetate fraction (IC<sub>50</sub> = 0.01 ± 0.00 mg/mL) demonstrated stronger inhibitory activity against α-glucosidase than acarbose (IC<sub>50</sub> = 0.27 ± 0.13 mg/mL). Phytochemical testing showed that the methanol extract and the ethyl acetate fraction contained secondary metabolites: alkaloids, flavonoids, steroids, and phenol hydroquinone. The compounds in methanol extract predicted to have inhibitory activity against α-amylase and α-glucosidase were Docosanol, Neophytadiene, Stigmasta-7,22-dien-3-ol,acetate,(3.beta.,5.alpha.,22E), Octadecanoic acid,2-oxo-,methyl ester, and phytol, while those in the ethyl acetate fraction were n-Nonadecane, Phytol, Butyl ester, 14-.Beta.-H-pregna, Octadecenoic acid, and Oleic acid. |
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| language | English |
| last_indexed | 2024-03-09T11:24:44Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-be10ec1763944b7faeece342896df4e82023-12-01T00:06:09ZengMDPI AGPlants2223-77472023-01-0112239310.3390/plants12020393In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, AcehMohamad Gazali0Odi Jolanda1Amir Husni2Nurjanah3Fadzilah Adibah Abd Majid4Zuriat5Rina Syafitri6Department of Marine Science, Faculty of Fisheries and Marine Science, Teuku Umar University, Aceh 23681, IndonesiaDepartment of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Aquatic Product Technology, Faculty of Fisheries and Marine Science, IPB University, Bogor 16680, IndonesiaInstitute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu 21030, MalaysiaDepartment of Fisheries, Faculty of Fisheries and Marine Science, Teuku Umar University, Aceh 23681, IndonesiaDepartment of Agribusiness, Faculty of Agriculture, Teuku Umar University, Aceh 23681, IndonesiaSeaweed belongs to marine biota and contains nutrients and secondary metabolites beneficial for health. This study aimed to determine the antidiabetic activity of extracts and fractions of green seaweed <i>Halimeda tuna</i>. The <i>H. tuna</i> sample was extracted with the maceration method using methanol and then partitioned using ethyl acetate and water to obtain ethyl acetate and water fractions. The methanol extract, ethyl acetate fraction, and water fraction of <i>H. tuna</i> were tested for their inhibitory activity against α-amilase and α-glucosidase. The methanol extract and the fractions with the highest inhibitory activity were phytochemically tested and analyzed using gas chromatography–mass spectrometry (GC-MS). The results showed that the ethyl acetate fraction (IC<sub>50</sub> = 0.88 ± 0.20 mg/mL) inhibited α-amylase relatively similar to acarbose (IC<sub>50</sub> = 0.76 ± 0.04 mg/mL). The methanol extract (IC<sub>50</sub> = 0.05 ± 0.01 mg/mL) and the ethyl acetate fraction (IC<sub>50</sub> = 0.01 ± 0.00 mg/mL) demonstrated stronger inhibitory activity against α-glucosidase than acarbose (IC<sub>50</sub> = 0.27 ± 0.13 mg/mL). Phytochemical testing showed that the methanol extract and the ethyl acetate fraction contained secondary metabolites: alkaloids, flavonoids, steroids, and phenol hydroquinone. The compounds in methanol extract predicted to have inhibitory activity against α-amylase and α-glucosidase were Docosanol, Neophytadiene, Stigmasta-7,22-dien-3-ol,acetate,(3.beta.,5.alpha.,22E), Octadecanoic acid,2-oxo-,methyl ester, and phytol, while those in the ethyl acetate fraction were n-Nonadecane, Phytol, Butyl ester, 14-.Beta.-H-pregna, Octadecenoic acid, and Oleic acid.https://www.mdpi.com/2223-7747/12/2/393<i>Halimeda tuna</i>α-amylaseα-glucosidasemethanol extractethyl acetate fraction |
| spellingShingle | Mohamad Gazali Odi Jolanda Amir Husni Nurjanah Fadzilah Adibah Abd Majid Zuriat Rina Syafitri In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh Plants <i>Halimeda tuna</i> α-amylase α-glucosidase methanol extract ethyl acetate fraction |
| title | In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh |
| title_full | In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh |
| title_fullStr | In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh |
| title_full_unstemmed | In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh |
| title_short | In Vitro α-Amylase and α-Glucosidase Inhibitory Activity of Green Seaweed <i>Halimeda tuna</i> Extract from the Coast of Lhok Bubon, Aceh |
| title_sort | in vitro α amylase and α glucosidase inhibitory activity of green seaweed i halimeda tuna i extract from the coast of lhok bubon aceh |
| topic | <i>Halimeda tuna</i> α-amylase α-glucosidase methanol extract ethyl acetate fraction |
| url | https://www.mdpi.com/2223-7747/12/2/393 |
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