Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai
Background: The increase in incidence of Methicillin Resistant Staphyloccocus aureus (MRSA) and its extraordinary potential to develop antimicrobial resistance has highlighted the need for better agents to treat such infections. This has led to a renewed interest in use of new drugs for treatme...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Krishna Vishwa Vidyapeeth (Deemed to be University), Karad
2015-01-01
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| Series: | Journal of Krishna Institute of Medical Sciences University |
| Subjects: | |
| Online Access: | http://jkimsu.com/jkimsu-vol4no3/JKIMSU,%20Vol.%204,%20No.%203,%20July-Sept%202015%20Page%2010-16.pdf |
| _version_ | 1827092837896814592 |
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| author | Arunagiri Subramanian Vidushi Chitalia Shashikant P. Vaidya Rajas V. Warke Abhay Chowdhary Ranjana A. Deshmukh |
| author_facet | Arunagiri Subramanian Vidushi Chitalia Shashikant P. Vaidya Rajas V. Warke Abhay Chowdhary Ranjana A. Deshmukh |
| author_sort | Arunagiri Subramanian |
| collection | DOAJ |
| description | Background: The increase in incidence of Methicillin
Resistant Staphyloccocus aureus (MRSA) and its
extraordinary potential to develop antimicrobial
resistance has highlighted the need for better agents to
treat such infections. This has led to a renewed interest
in use of new drugs for treatment with clindamycin and
quinuprsitin-dalfopristin being the preferred choice for
treatment. Aim & Objectives: This study was undertaken
to detect the prevalence of MacrolideLincosamide-Streptogramin
(MLS) resistance among clinical isolates of MRSA.Material and Methods:Two
hundred and thirty clinical isolates of S. aureus were
subjected to routine antibiotic susceptibility testing
including cefoxitin, erythromycin and quinupristindalfopristin.
Inducible resistance to clindamycin was
tested by 'D' test as per Clinical and Laboratory
Standards Institute (CLSI) guidelines. Results: Out of
all S. aureus isolates, 93.91% were identified as
MRSA. In the disc diffusion testing, 81.5% of isolates
showed erythromycin resistance. Among these, the
prevalence of constitutive (cMLS ), inducible (iMLS ) b b
and MS-phenotype were 35.80%, 31.82% and 32.39%
respectively by the D-test method. 77.8% of isolates
were resistant to quinupristin-dalfopristin and the
Minimum Inhibitory Concentration (MIC) ranged
from 4–32 µg/ml. 89.20% of isolates were resistant to
both quinupristin-dalfopristin and erythromycin of
which 35.03%, 35.67% and 29.30% belonged to
iMLS , cMLS and MS phenotype respectively. Conclusion: The emergence of quinupristindalfopristin
resistance and MLS phenotypes brings b
about the need for the simple and reliable D-test in
routine diagnosis and further susceptibility testing for
proper antimicrobial therapy. |
| first_indexed | 2024-04-12T16:29:33Z |
| format | Article |
| id | doaj.art-be1236ffb5d24f41a7e625a398441278 |
| institution | Directory Open Access Journal |
| issn | 2231-4261 2231-4261 |
| language | English |
| last_indexed | 2025-03-20T06:18:33Z |
| publishDate | 2015-01-01 |
| publisher | Krishna Vishwa Vidyapeeth (Deemed to be University), Karad |
| record_format | Article |
| series | Journal of Krishna Institute of Medical Sciences University |
| spelling | doaj.art-be1236ffb5d24f41a7e625a3984412782024-10-02T16:35:27ZengKrishna Vishwa Vidyapeeth (Deemed to be University), KaradJournal of Krishna Institute of Medical Sciences University2231-42612231-42612015-01-01431016Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in MumbaiArunagiri Subramanian0Vidushi Chitalia1Shashikant P. Vaidya2Rajas V. Warke3Abhay Chowdhary4 Ranjana A. Deshmukh5Department of Clinical Pathology, Haffkine Institute, Acharya Donde Marg, Parel, Mumbai- 400012 (Maharashtra), India,Department of Clinical Pathology, Haffkine Institute, Acharya Donde Marg, Parel, Mumbai- 400012 (Maharashtra), India,Department of Clinical Pathology, Haffkine Institute, Acharya Donde Marg, Parel, Mumbai- 400012 (Maharashtra), India,Department of Molecular Biology, HiMedia Laboratories, Vadhani Industrial Estate, Lal Bahadur Shastri Marg, Mumbai- 400070 (Maharashtra) India,Department of Virology & Immunology, Haffkine Institute, Acharya Donde Marg, Parel, Mumbai-400 012 (Maharashtra) IndiaDepartment of Virology & Immunology, Haffkine Institute, Acharya Donde Marg, Parel, Mumbai-400 012 (Maharashtra) IndiaBackground: The increase in incidence of Methicillin Resistant Staphyloccocus aureus (MRSA) and its extraordinary potential to develop antimicrobial resistance has highlighted the need for better agents to treat such infections. This has led to a renewed interest in use of new drugs for treatment with clindamycin and quinuprsitin-dalfopristin being the preferred choice for treatment. Aim & Objectives: This study was undertaken to detect the prevalence of MacrolideLincosamide-Streptogramin (MLS) resistance among clinical isolates of MRSA.Material and Methods:Two hundred and thirty clinical isolates of S. aureus were subjected to routine antibiotic susceptibility testing including cefoxitin, erythromycin and quinupristindalfopristin. Inducible resistance to clindamycin was tested by 'D' test as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: Out of all S. aureus isolates, 93.91% were identified as MRSA. In the disc diffusion testing, 81.5% of isolates showed erythromycin resistance. Among these, the prevalence of constitutive (cMLS ), inducible (iMLS ) b b and MS-phenotype were 35.80%, 31.82% and 32.39% respectively by the D-test method. 77.8% of isolates were resistant to quinupristin-dalfopristin and the Minimum Inhibitory Concentration (MIC) ranged from 4–32 µg/ml. 89.20% of isolates were resistant to both quinupristin-dalfopristin and erythromycin of which 35.03%, 35.67% and 29.30% belonged to iMLS , cMLS and MS phenotype respectively. Conclusion: The emergence of quinupristindalfopristin resistance and MLS phenotypes brings b about the need for the simple and reliable D-test in routine diagnosis and further susceptibility testing for proper antimicrobial therapy.http://jkimsu.com/jkimsu-vol4no3/JKIMSU,%20Vol.%204,%20No.%203,%20July-Sept%202015%20Page%2010-16.pdfClindamycinD-testGlycopeptidesInducible ResistanceQuinupristin-dalfopristin |
| spellingShingle | Arunagiri Subramanian Vidushi Chitalia Shashikant P. Vaidya Rajas V. Warke Abhay Chowdhary Ranjana A. Deshmukh Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai Journal of Krishna Institute of Medical Sciences University Clindamycin D-test Glycopeptides Inducible Resistance Quinupristin-dalfopristin |
| title | Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai |
| title_full | Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai |
| title_fullStr | Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai |
| title_full_unstemmed | Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai |
| title_short | Detection of Macrolide, Lincosamide and Streptogramin Resistance among Methicillin Resistant Staphylococcus aureus (MRSA) in Mumbai |
| title_sort | detection of macrolide lincosamide and streptogramin resistance among methicillin resistant staphylococcus aureus mrsa in mumbai |
| topic | Clindamycin D-test Glycopeptides Inducible Resistance Quinupristin-dalfopristin |
| url | http://jkimsu.com/jkimsu-vol4no3/JKIMSU,%20Vol.%204,%20No.%203,%20July-Sept%202015%20Page%2010-16.pdf |
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