Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus)
The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes re...
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Sociedade Brasileira de Genética
2006-01-01
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Series: | Genetics and Molecular Biology |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012 |
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author | Eileen Armstrong Alicia Postiglioni Amparo Martínez Gonzalo Rincón José Luis Vega-Pla |
author_facet | Eileen Armstrong Alicia Postiglioni Amparo Martínez Gonzalo Rincón José Luis Vega-Pla |
author_sort | Eileen Armstrong |
collection | DOAJ |
description | The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity was 0.623 and the polymorphic information content (PIC) was between 0.266 for HEL13 and 0.794 for CSSM66. The genetic diversity found in polymorphic markers in the breeding bulls of this Creole cattle population supports previous genetic analyses using major production genes and indicate that further studies should be carried out on this population to provide data of interest to cattle production. |
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issn | 1415-4757 1678-4685 |
language | English |
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publishDate | 2006-01-01 |
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series | Genetics and Molecular Biology |
spelling | doaj.art-be128af9cbd1463c858d848d48c271072022-12-22T00:55:09ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129226727210.1590/S1415-47572006000200012Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus)Eileen ArmstrongAlicia PostiglioniAmparo MartínezGonzalo RincónJosé Luis Vega-PlaThe Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity was 0.623 and the polymorphic information content (PIC) was between 0.266 for HEL13 and 0.794 for CSSM66. The genetic diversity found in polymorphic markers in the breeding bulls of this Creole cattle population supports previous genetic analyses using major production genes and indicate that further studies should be carried out on this population to provide data of interest to cattle production.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012Uruguayan Creole cattlegenetic diversitymicrosatellites |
spellingShingle | Eileen Armstrong Alicia Postiglioni Amparo Martínez Gonzalo Rincón José Luis Vega-Pla Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) Genetics and Molecular Biology Uruguayan Creole cattle genetic diversity microsatellites |
title | Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) |
title_full | Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) |
title_fullStr | Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) |
title_full_unstemmed | Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) |
title_short | Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus) |
title_sort | microsatellite analysis of a sample of uruguayan creole bulls bos taurus |
topic | Uruguayan Creole cattle genetic diversity microsatellites |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012 |
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