Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia
Background/Aims: It is known that mesenchymal stem cells (MSCs) can have variable responses to hypoxic conditions and that hypoxia may specifically stimulate differentiation into osteogenic, chondrogenic, or adipogenic cells. Based on our previous study, we hypothesized that hypoxia may also induce...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2016-09-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/447861 |
| _version_ | 1818516200520417280 |
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| author | Jung-Won Choi Kyung-Eun Kim Chang Youn Lee Jiyun Lee Hyang-Hee Seo Kyu Hee Lim Eunhyun Choi Soyeon Lim Seahyong Lee Sang Woo Kim Ki-Chul Hwang |
| author_facet | Jung-Won Choi Kyung-Eun Kim Chang Youn Lee Jiyun Lee Hyang-Hee Seo Kyu Hee Lim Eunhyun Choi Soyeon Lim Seahyong Lee Sang Woo Kim Ki-Chul Hwang |
| author_sort | Jung-Won Choi |
| collection | DOAJ |
| description | Background/Aims: It is known that mesenchymal stem cells (MSCs) can have variable responses to hypoxic conditions and that hypoxia may specifically stimulate differentiation into osteogenic, chondrogenic, or adipogenic cells. Based on our previous study, we hypothesized that hypoxia may also induce MSC differentiation into cardiomyocytes and/or cells with comparable phenotypes. Methods: The differences in the proteomes were specifically investigated in bone marrow-derived rat MSCs (BM-rMSCs) under normoxic and hypoxic conditions using 2-DE combined with a MALDI-TOF-MS analysis and western blot analysis. In addition, genetic and/or proteomic interactions were assessed using a String network analysis. Results: Among the 35 markedly changed spots from a total of 393 matched spots, 24 were highly up-regulated and 11 were significantly down-regulated in hypoxic rMSCs based on a proteomic analysis. Although hypoxia failed to induce the direct differentiation of rMSCs into cardiomyocytes, several cardiomyocyte differentiation-related genes and proteins were significantly increased by hypoxic stress. Conclusion: We found that BM-rMSCs alter their expression of several cardiomyocyte differentiation-related genes and proteins under hypoxic conditions, and we examined the interactions between these genes and/or proteins, providing new insights for the applicability of MSCs preconditioned by hypoxic stimulation for use in cardiac diseases. |
| first_indexed | 2024-12-11T00:38:55Z |
| format | Article |
| id | doaj.art-be1546cab6e54d19bbdbc94369726b53 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-11T00:38:55Z |
| publishDate | 2016-09-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-be1546cab6e54d19bbdbc94369726b532022-12-22T01:27:01ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-09-013941595160710.1159/000447861447861Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to HypoxiaJung-Won ChoiKyung-Eun KimChang Youn LeeJiyun LeeHyang-Hee SeoKyu Hee LimEunhyun ChoiSoyeon LimSeahyong LeeSang Woo KimKi-Chul HwangBackground/Aims: It is known that mesenchymal stem cells (MSCs) can have variable responses to hypoxic conditions and that hypoxia may specifically stimulate differentiation into osteogenic, chondrogenic, or adipogenic cells. Based on our previous study, we hypothesized that hypoxia may also induce MSC differentiation into cardiomyocytes and/or cells with comparable phenotypes. Methods: The differences in the proteomes were specifically investigated in bone marrow-derived rat MSCs (BM-rMSCs) under normoxic and hypoxic conditions using 2-DE combined with a MALDI-TOF-MS analysis and western blot analysis. In addition, genetic and/or proteomic interactions were assessed using a String network analysis. Results: Among the 35 markedly changed spots from a total of 393 matched spots, 24 were highly up-regulated and 11 were significantly down-regulated in hypoxic rMSCs based on a proteomic analysis. Although hypoxia failed to induce the direct differentiation of rMSCs into cardiomyocytes, several cardiomyocyte differentiation-related genes and proteins were significantly increased by hypoxic stress. Conclusion: We found that BM-rMSCs alter their expression of several cardiomyocyte differentiation-related genes and proteins under hypoxic conditions, and we examined the interactions between these genes and/or proteins, providing new insights for the applicability of MSCs preconditioned by hypoxic stimulation for use in cardiac diseases.http://www.karger.com/Article/FullText/447861Bone marrow-derived rat mesenchymal stem cellsHypoxiaCardiomyocyte differentiationTwo-dimensional electrophoresisNetwork analysis |
| spellingShingle | Jung-Won Choi Kyung-Eun Kim Chang Youn Lee Jiyun Lee Hyang-Hee Seo Kyu Hee Lim Eunhyun Choi Soyeon Lim Seahyong Lee Sang Woo Kim Ki-Chul Hwang Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia Cellular Physiology and Biochemistry Bone marrow-derived rat mesenchymal stem cells Hypoxia Cardiomyocyte differentiation Two-dimensional electrophoresis Network analysis |
| title | Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia |
| title_full | Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia |
| title_fullStr | Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia |
| title_full_unstemmed | Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia |
| title_short | Alterations in Cardiomyocyte Differentiation-Related Proteins in Rat Mesenchymal Stem Cells Exposed to Hypoxia |
| title_sort | alterations in cardiomyocyte differentiation related proteins in rat mesenchymal stem cells exposed to hypoxia |
| topic | Bone marrow-derived rat mesenchymal stem cells Hypoxia Cardiomyocyte differentiation Two-dimensional electrophoresis Network analysis |
| url | http://www.karger.com/Article/FullText/447861 |
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