S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress
Nitric oxide (NO) modulates plant response by post-translationally modifying proteins, mainly through S-nitrosylation. Ascorbate peroxidase (APX) in the ascorbate-glutathione (AsA-GSH) cycle participates in the removal of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Howeve...
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MDPI AG
2023-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/5/1322 |
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| author | Chuntao Lv Yuanlin Liang Manqi Wang Kunzhi Li Xudong Sun Huini Xu |
| author_facet | Chuntao Lv Yuanlin Liang Manqi Wang Kunzhi Li Xudong Sun Huini Xu |
| author_sort | Chuntao Lv |
| collection | DOAJ |
| description | Nitric oxide (NO) modulates plant response by post-translationally modifying proteins, mainly through S-nitrosylation. Ascorbate peroxidase (APX) in the ascorbate-glutathione (AsA-GSH) cycle participates in the removal of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). However, the relationship between S-nitrosylation and the role of tomato APX (<i>SlAPX</i>) under nitrate stress is still unclear. In this study, the enzyme activity, mRNA expression, and S-nitrosylation level of <i>SlAPX</i> were significantly increased in tomato roots after nitrate treatment. <i>SlAPX</i> protein could be S-nitrosylated by S-nitrosoglutathione in vitro, and APX activity was significantly increased after S-nitrosylation. The <i>SlAPX</i> overexpressed tobacco plants grew better than the wild type (WT) plants under nitrate stress. Meanwhile, the transgenic plants showed lower reactive oxygen species and malondialdehyde content, higher APX, monodehydroascorbate reductase, glutathione reductase activities, ascorbic acid/dehydroascorbic acid, and reduced glutathione/oxidized glutathione ratio, proline, and soluble sugar contents than those in the WT plants under nitrate treatment. Moreover, overexpressed transgenic seeds showed higher tolerance to methyl viologen induced oxidative stress compared with the WT. The NO accumulation and S-nitrosylation APX level were higher in transgenic plants than in WT plants after nitrate stress treatment. Our results provide novel insights into the mechanism of <i>SlAPX</i> modulation excess nitrate stress tolerance involving the S-nitrosylation modification. |
| first_indexed | 2024-03-11T04:01:56Z |
| format | Article |
| id | doaj.art-be1701a03b12489ea0eba017425a50ac |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T04:01:56Z |
| publishDate | 2023-05-01 |
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| series | Agronomy |
| spelling | doaj.art-be1701a03b12489ea0eba017425a50ac2023-11-18T00:06:31ZengMDPI AGAgronomy2073-43952023-05-01135132210.3390/agronomy13051322S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate StressChuntao Lv0Yuanlin Liang1Manqi Wang2Kunzhi Li3Xudong Sun4Huini Xu5Faculty of Life Science and Technology, Kunming University of Science and Technology, Jingming South Street, Kunming 650224, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Jingming South Street, Kunming 650224, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Jingming South Street, Kunming 650224, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Jingming South Street, Kunming 650224, ChinaKey Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Jingming South Street, Kunming 650224, ChinaNitric oxide (NO) modulates plant response by post-translationally modifying proteins, mainly through S-nitrosylation. Ascorbate peroxidase (APX) in the ascorbate-glutathione (AsA-GSH) cycle participates in the removal of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). However, the relationship between S-nitrosylation and the role of tomato APX (<i>SlAPX</i>) under nitrate stress is still unclear. In this study, the enzyme activity, mRNA expression, and S-nitrosylation level of <i>SlAPX</i> were significantly increased in tomato roots after nitrate treatment. <i>SlAPX</i> protein could be S-nitrosylated by S-nitrosoglutathione in vitro, and APX activity was significantly increased after S-nitrosylation. The <i>SlAPX</i> overexpressed tobacco plants grew better than the wild type (WT) plants under nitrate stress. Meanwhile, the transgenic plants showed lower reactive oxygen species and malondialdehyde content, higher APX, monodehydroascorbate reductase, glutathione reductase activities, ascorbic acid/dehydroascorbic acid, and reduced glutathione/oxidized glutathione ratio, proline, and soluble sugar contents than those in the WT plants under nitrate treatment. Moreover, overexpressed transgenic seeds showed higher tolerance to methyl viologen induced oxidative stress compared with the WT. The NO accumulation and S-nitrosylation APX level were higher in transgenic plants than in WT plants after nitrate stress treatment. Our results provide novel insights into the mechanism of <i>SlAPX</i> modulation excess nitrate stress tolerance involving the S-nitrosylation modification.https://www.mdpi.com/2073-4395/13/5/1322ascorbate peroxidaseNOAsA-GSH cycletomato |
| spellingShingle | Chuntao Lv Yuanlin Liang Manqi Wang Kunzhi Li Xudong Sun Huini Xu S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress Agronomy ascorbate peroxidase NO AsA-GSH cycle tomato |
| title | S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress |
| title_full | S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress |
| title_fullStr | S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress |
| title_full_unstemmed | S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress |
| title_short | S-nitrosylation of <i>SlAPX</i> Is Involved in Alleviating Oxidative Damage in Transgenic Tobacco under Nitrate Stress |
| title_sort | s nitrosylation of i slapx i is involved in alleviating oxidative damage in transgenic tobacco under nitrate stress |
| topic | ascorbate peroxidase NO AsA-GSH cycle tomato |
| url | https://www.mdpi.com/2073-4395/13/5/1322 |
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