In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy
The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates...
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MDPI AG
2014-05-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/12/5/2827 |
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| author | Rimi Miyaoka Masahito Hosokawa Masahiro Ando Tetsushi Mori Hiro-o Hamaguchi Haruko Takeyama |
| author_facet | Rimi Miyaoka Masahito Hosokawa Masahiro Ando Tetsushi Mori Hiro-o Hamaguchi Haruko Takeyama |
| author_sort | Rimi Miyaoka |
| collection | DOAJ |
| description | The study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes—Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling. |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T05:21:55Z |
| publishDate | 2014-05-01 |
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| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-be173fd7ff1f44dfbd41e8d185f7acef2022-12-22T02:10:09ZengMDPI AGMarine Drugs1660-33972014-05-011252827283910.3390/md12052827md12052827In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman MicrospectroscopyRimi Miyaoka0Masahito Hosokawa1Masahiro Ando2Tetsushi Mori3Hiro-o Hamaguchi4Haruko Takeyama5Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, JapanDepartment of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, JapanConsolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513, Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, JapanDepartment of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, JapanConsolidated Research Institute for Advanced Science and Medical Care, Waseda University, 513, Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, JapanDepartment of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, JapanThe study of spatial distribution of secondary metabolites within microbial cells facilitates the screening of candidate strains from marine environments for functional metabolites and allows for the subsequent assessment of the production of metabolites, such as antibiotics. This paper demonstrates the first application of Raman microspectroscopy for in situ detection of the antifungal antibiotic amphotericin B (AmB) produced by actinomycetes—Streptomyces nodosus. Raman spectra measured from hyphae of S. nodosus show the specific Raman bands, caused by resonance enhancement, corresponding to the polyene chain of AmB. In addition, Raman microspectroscopy enabled us to monitor the time-dependent change of AmB production corresponding to the growth of mycelia. The Raman images of S. nodosus reveal the heterogeneous distribution of AmB within the mycelia and individual hyphae. Moreover, the molecular association state of AmB in the mycelia was directly identified by observed Raman spectral shifts. These findings suggest that Raman microspectroscopy could be used for in situ monitoring of antibiotic production directly in marine microorganisms with a method that is non-destructive and does not require labeling.http://www.mdpi.com/1660-3397/12/5/2827Raman microspectroscopy in situ detectionantibioticssecondary metabolitesactinomycetes |
| spellingShingle | Rimi Miyaoka Masahito Hosokawa Masahiro Ando Tetsushi Mori Hiro-o Hamaguchi Haruko Takeyama In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy Marine Drugs Raman microspectroscopy in situ detection antibiotics secondary metabolites actinomycetes |
| title | In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy |
| title_full | In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy |
| title_fullStr | In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy |
| title_full_unstemmed | In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy |
| title_short | In Situ Detection of Antibiotic Amphotericin B Produced in Streptomyces nodosus Using Raman Microspectroscopy |
| title_sort | in situ detection of antibiotic amphotericin b produced in streptomyces nodosus using raman microspectroscopy |
| topic | Raman microspectroscopy in situ detection antibiotics secondary metabolites actinomycetes |
| url | http://www.mdpi.com/1660-3397/12/5/2827 |
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