Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium
Lead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1335854/full |
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| author | Hossam S. El-Beltagi Hossam S. El-Beltagi Asmaa A. Halema Zainab M. Almutairi Hayfa Habes Almutairi Nagwa I. Elarabi Abdelhadi A. Abdelhadi Ahmed R. Henawy Heba A. R. Abdelhaleem |
| author_facet | Hossam S. El-Beltagi Hossam S. El-Beltagi Asmaa A. Halema Zainab M. Almutairi Hayfa Habes Almutairi Nagwa I. Elarabi Abdelhadi A. Abdelhadi Ahmed R. Henawy Heba A. R. Abdelhaleem |
| author_sort | Hossam S. El-Beltagi |
| collection | DOAJ |
| description | Lead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated by heavily lead-containing industries. Eight lead-resistant strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as a particularly promising candidate, demonstrating an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight. Furthermore, E. kobei FACU6 displayed a remarkable a maximum tolerance concentration (MTC) for lead reaching 3,000 mg/L. To further investigate the morphological changes in E. kobei FACU6 in response to lead exposure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. These analyses revealed significant lead adsorption and intracellular accumulation in treated bacteria in contrast to the control bacterium. Whole-genome sequencing was performed to gain deeper insights into E. kobei’s lead resistance mechanisms. Structural annotation revealed a genome size of 4,856,454 bp, with a G + C content of 55.06%. The genome encodes 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. Notably, genes associated with heavy metal resistance and their corresponding regulatory elements were identified within the genome. Furthermore, the expression levels of four specific heavy metal resistance genes were evaluated. Our findings revealed a statistically significant upregulation in gene expression under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals. The outstanding potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion makes it a valuable candidate for developing safe and effective strategies for heavy metal disposal. |
| first_indexed | 2024-03-08T16:07:43Z |
| format | Article |
| id | doaj.art-be178089181a427ca3e03dd967762a0e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T16:07:43Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-be178089181a427ca3e03dd967762a0e2024-01-08T04:46:29ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-01-011110.3389/fbioe.2023.13358541335854Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacteriumHossam S. El-Beltagi0Hossam S. El-Beltagi1Asmaa A. Halema2Zainab M. Almutairi3Hayfa Habes Almutairi4Nagwa I. Elarabi5Abdelhadi A. Abdelhadi6Ahmed R. Henawy7Heba A. R. Abdelhaleem8Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiBiochemistry Department, Faculty of Agriculture, Cairo University, Giza, EgyptGenetics Department, Faculty of Agriculture, Cairo University, Giza, EgyptBiology Department, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi ArabiaDepartment of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi ArabiaGenetics Department, Faculty of Agriculture, Cairo University, Giza, EgyptGenetics Department, Faculty of Agriculture, Cairo University, Giza, EgyptMicrobiology Department, Faculty of Agriculture, Cairo University, Giza, EgyptCollege of Biotechnology, Misr University for Science and Technology (MUST), 6th October City, EgyptLead pollution of the environment poses a major global threat to the ecosystem. Bacterial bioremediation offers a promising alternative to traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated by heavily lead-containing industries. Eight lead-resistant strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6 emerged as a particularly promising candidate, demonstrating an efficient lead removal rate of 83.4% and a remarkable lead absorption capacity of 571.9 mg/g dry weight. Furthermore, E. kobei FACU6 displayed a remarkable a maximum tolerance concentration (MTC) for lead reaching 3,000 mg/L. To further investigate the morphological changes in E. kobei FACU6 in response to lead exposure, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were employed. These analyses revealed significant lead adsorption and intracellular accumulation in treated bacteria in contrast to the control bacterium. Whole-genome sequencing was performed to gain deeper insights into E. kobei’s lead resistance mechanisms. Structural annotation revealed a genome size of 4,856,454 bp, with a G + C content of 55.06%. The genome encodes 4,655 coding sequences (CDS), 75 tRNA genes, and 4 rRNA genes. Notably, genes associated with heavy metal resistance and their corresponding regulatory elements were identified within the genome. Furthermore, the expression levels of four specific heavy metal resistance genes were evaluated. Our findings revealed a statistically significant upregulation in gene expression under specific environmental conditions, including pH 7, temperature of 30°C, and high concentrations of heavy metals. The outstanding potential of E. kobei FACU6 as a source of diverse genes related to heavy metal resistance and plant growth promotion makes it a valuable candidate for developing safe and effective strategies for heavy metal disposal.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1335854/fullbioremediationgenome analysisheavy metal resistant bacterialeadqPCRTEM |
| spellingShingle | Hossam S. El-Beltagi Hossam S. El-Beltagi Asmaa A. Halema Zainab M. Almutairi Hayfa Habes Almutairi Nagwa I. Elarabi Abdelhadi A. Abdelhadi Ahmed R. Henawy Heba A. R. Abdelhaleem Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium Frontiers in Bioengineering and Biotechnology bioremediation genome analysis heavy metal resistant bacteria lead qPCR TEM |
| title | Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium |
| title_full | Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium |
| title_fullStr | Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium |
| title_full_unstemmed | Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium |
| title_short | Draft genome analysis for Enterobacter kobei, a promising lead bioremediation bacterium |
| title_sort | draft genome analysis for enterobacter kobei a promising lead bioremediation bacterium |
| topic | bioremediation genome analysis heavy metal resistant bacteria lead qPCR TEM |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1335854/full |
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