A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2001-08-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012 |
| _version_ | 1819236546743631872 |
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| author | J.M. Schneedorf M.M. Santoro R.E. Lovrien M. Mares-Guia |
| author_facet | J.M. Schneedorf M.M. Santoro R.E. Lovrien M. Mares-Guia |
| author_sort | J.M. Schneedorf |
| collection | DOAJ |
| description | Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the macromolecule. |
| first_indexed | 2024-12-23T13:06:10Z |
| format | Article |
| id | doaj.art-be1a004166cf45259079f7e197e2ef19 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-23T13:06:10Z |
| publishDate | 2001-08-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-be1a004166cf45259079f7e197e2ef192022-12-21T17:45:52ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2001-08-013481047105410.1590/S0100-879X2001000800012A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid mediaJ.M. SchneedorfM.M. SantoroR.E. LovrienM. Mares-GuiaSynthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysis using bovine trypsin and flavianic acid as a model able to cause coprecipitation. A two-step mode of interaction was found to be dependent on the incubation periods allowed for binding, with pronounced binding occurring after 42 h of incubation. The first step seems to involve hydration effects and conformational changes induced by binding of the first dye molecule, following rapid denaturation due to the binding of six additional flavianate anions to the macromolecule.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012dye bindingbinding isothermcoprecipitation |
| spellingShingle | J.M. Schneedorf M.M. Santoro R.E. Lovrien M. Mares-Guia A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media Brazilian Journal of Medical and Biological Research dye binding binding isotherm coprecipitation |
| title | A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media |
| title_full | A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media |
| title_fullStr | A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media |
| title_full_unstemmed | A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media |
| title_short | A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media |
| title_sort | time dependent two step binding mode of the nitro dye flavianic acid to trypsin in acid media |
| topic | dye binding binding isotherm coprecipitation |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012 |
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