A time-dependent, two-step binding mode of the nitro dye flavianic acid to trypsin in acid media
Synthetic dyes bind to proteins causing selective coprecipitation of the complexes in acid aqueous solution by a process of reversible denaturation that can be used as an alternative method for protein fractionation. The events that occur before precipitation were investigated by equilibrium dialysi...
Main Authors: | J.M. Schneedorf, M.M. Santoro, R.E. Lovrien, M. Mares-Guia |
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Format: | Article |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2001-08-01
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Series: | Brazilian Journal of Medical and Biological Research |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800012 |
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