Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study
Nikodimos Eshetu Dabe,1 Adane Teshome Kefale,2 Tegene Legese Dadi3 1Department of Biomedical Science, College of Health Sciences, Mizan–Tepi University, Mizan Teferi, Ethiopia; 2Department of Pharmacy, College of Health Sciences, Debre Berhan University, Debre Berhan, Ethiopia; 3Department...
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Dove Medical Press
2020-07-01
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| Series: | Journal of Experimental Pharmacology |
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| Online Access: | https://www.dovepress.com/evaluation-of-abortifacient-effect-of-rumex-nepalensis-spreng-among-pr-peer-reviewed-fulltext-article-JEP |
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| author | Dabe NE Kefale AT Dadi TL |
| author_facet | Dabe NE Kefale AT Dadi TL |
| author_sort | Dabe NE |
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| description | Nikodimos Eshetu Dabe,1 Adane Teshome Kefale,2 Tegene Legese Dadi3 1Department of Biomedical Science, College of Health Sciences, Mizan–Tepi University, Mizan Teferi, Ethiopia; 2Department of Pharmacy, College of Health Sciences, Debre Berhan University, Debre Berhan, Ethiopia; 3Department of Public Health, College of Health Sciences, Mizan–Tepi University, Mizan Teferi, EthiopiaCorrespondence: Nikodimos Eshetu Dabe Email nixon.eshetu@gmail.comBackground: Rumex nepalensis Spreng (Amharic: Yewsha Tult) belongs to the Polygonaceae (buckwheat) family. In Ethiopia, the plant is traditionally used for the treatment of stomach ache, tonsillitis, ascariasis, uterine bleeding, etc. An ethnobotanical study from Mizan–Tepi University also reported the use of the plant by “Shekicho” people as an abortifacient. As a result, this study aimed at the assessment of the outcome of hydro-ethanolic leaves extract of R. nepalensis on Swiss albino pregnant rats and confirm its abortifacient activity.Methods: The hydro-alcoholic leaves extract of Rumex nepalensis Spreng was evaluated for its abortifacient activity in Swiss albino rats. The mature female rats were mated overnight to male rats in mating cages. Two different dosage regimens (300 mg/kg, 600 mg/kg) of the extract were administered. Laparotomy was performed on the rats to assess the uterus and ovary, the viable, non-viable, adsorbing sites, and corpora lutea. Differences between the experimental and control groups were compared using one-way analysis of variance (ANOVA), followed by Dunnett’s T-test to determine their level of significance.Results and Discussion: This study revealed that Rumex nepalensis Spreng had anti-implantation and abortifacient activities at both 300 and 600 mg/kg doses, which was statistically significant as compared with the controls. It was relatively safe up to the dose of 5000 mg/kg, where no mortality and organ toxicity were manifested. Phytochemicals identified were alkaloids, flavonoids, saponins, tannins, steroids, and anthraquinones.Conclusion: In general, our study showed that R. nepalensis had a significant abortifacient activity that testifies its traditional dibs. Therefore, the use of this plant should be avoided in pregnant women to minimize unintended abortion and further studies are needed to know its mechanism of activity and to identify the phytochemicals corresponding to this activity. Checking its efficacy on other species is also needed.Keywords: Rumex nepalensis Spreng, traditional medicine, abortifacient activity |
| first_indexed | 2024-12-19T20:59:59Z |
| format | Article |
| id | doaj.art-be1c98d60c7142a29786580d6961514d |
| institution | Directory Open Access Journal |
| issn | 1179-1454 |
| language | English |
| last_indexed | 2024-12-19T20:59:59Z |
| publishDate | 2020-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Experimental Pharmacology |
| spelling | doaj.art-be1c98d60c7142a29786580d6961514d2022-12-21T20:05:50ZengDove Medical PressJournal of Experimental Pharmacology1179-14542020-07-01Volume 1225526555841Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based StudyDabe NEKefale ATDadi TLNikodimos Eshetu Dabe,1 Adane Teshome Kefale,2 Tegene Legese Dadi3 1Department of Biomedical Science, College of Health Sciences, Mizan–Tepi University, Mizan Teferi, Ethiopia; 2Department of Pharmacy, College of Health Sciences, Debre Berhan University, Debre Berhan, Ethiopia; 3Department of Public Health, College of Health Sciences, Mizan–Tepi University, Mizan Teferi, EthiopiaCorrespondence: Nikodimos Eshetu Dabe Email nixon.eshetu@gmail.comBackground: Rumex nepalensis Spreng (Amharic: Yewsha Tult) belongs to the Polygonaceae (buckwheat) family. In Ethiopia, the plant is traditionally used for the treatment of stomach ache, tonsillitis, ascariasis, uterine bleeding, etc. An ethnobotanical study from Mizan–Tepi University also reported the use of the plant by “Shekicho” people as an abortifacient. As a result, this study aimed at the assessment of the outcome of hydro-ethanolic leaves extract of R. nepalensis on Swiss albino pregnant rats and confirm its abortifacient activity.Methods: The hydro-alcoholic leaves extract of Rumex nepalensis Spreng was evaluated for its abortifacient activity in Swiss albino rats. The mature female rats were mated overnight to male rats in mating cages. Two different dosage regimens (300 mg/kg, 600 mg/kg) of the extract were administered. Laparotomy was performed on the rats to assess the uterus and ovary, the viable, non-viable, adsorbing sites, and corpora lutea. Differences between the experimental and control groups were compared using one-way analysis of variance (ANOVA), followed by Dunnett’s T-test to determine their level of significance.Results and Discussion: This study revealed that Rumex nepalensis Spreng had anti-implantation and abortifacient activities at both 300 and 600 mg/kg doses, which was statistically significant as compared with the controls. It was relatively safe up to the dose of 5000 mg/kg, where no mortality and organ toxicity were manifested. Phytochemicals identified were alkaloids, flavonoids, saponins, tannins, steroids, and anthraquinones.Conclusion: In general, our study showed that R. nepalensis had a significant abortifacient activity that testifies its traditional dibs. Therefore, the use of this plant should be avoided in pregnant women to minimize unintended abortion and further studies are needed to know its mechanism of activity and to identify the phytochemicals corresponding to this activity. Checking its efficacy on other species is also needed.Keywords: Rumex nepalensis Spreng, traditional medicine, abortifacient activityhttps://www.dovepress.com/evaluation-of-abortifacient-effect-of-rumex-nepalensis-spreng-among-pr-peer-reviewed-fulltext-article-JEPrumex nepalensis sprengtraditional medicineabortifacient activity |
| spellingShingle | Dabe NE Kefale AT Dadi TL Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study Journal of Experimental Pharmacology rumex nepalensis spreng traditional medicine abortifacient activity |
| title | Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study |
| title_full | Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study |
| title_fullStr | Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study |
| title_full_unstemmed | Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study |
| title_short | Evaluation of Abortifacient Effect of Rumex nepalensis Spreng Among Pregnant Swiss Albino Rats: Laboratory-Based Study |
| title_sort | evaluation of abortifacient effect of rumex nepalensis spreng among pregnant swiss albino rats laboratory based study |
| topic | rumex nepalensis spreng traditional medicine abortifacient activity |
| url | https://www.dovepress.com/evaluation-of-abortifacient-effect-of-rumex-nepalensis-spreng-among-pr-peer-reviewed-fulltext-article-JEP |
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