Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma
<p>Abstract</p> <p>Background</p> <p>Succinate dehydrogenase (SDH) has been associated with carcinogenesis in pheochromocytoma and paraganglioma. In the present study we investigated components of the oxidative phosphorylation system in human neuroblastoma tissue sample...
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| Format: | Article |
| Language: | English |
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BMC
2010-04-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/10/149 |
| _version_ | 1818178081792196608 |
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| author | Feichtinger Rene' G Zimmermann Franz Mayr Johannes A Neureiter Daniel Hauser-Kronberger Cornelia Schilling Freimut H Jones Neil Sperl Wolfgang Kofler Barbara |
| author_facet | Feichtinger Rene' G Zimmermann Franz Mayr Johannes A Neureiter Daniel Hauser-Kronberger Cornelia Schilling Freimut H Jones Neil Sperl Wolfgang Kofler Barbara |
| author_sort | Feichtinger Rene' G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Succinate dehydrogenase (SDH) has been associated with carcinogenesis in pheochromocytoma and paraganglioma. In the present study we investigated components of the oxidative phosphorylation system in human neuroblastoma tissue samples.</p> <p>Methods</p> <p>Spectrophotometric measurements, immunohistochemical analysis and Western blot analysis were used to characterize the aerobic mitochondrial energy metabolism in neuroblastomas (NB).</p> <p>Results</p> <p>Compared to mitochondrial citrate synthase, SDH activity was severely reduced in NB (n = 14) versus kidney tissue. However no pathogenic mutations could be identified in any of the four subunits of SDH. Furthermore, no genetic alterations could be identified in the two novel SDH assembly factors SDHAF1 and SDH5. Alterations in genes encoding nfs-1, frataxin and isd-11 that could lead to a diminished SDH activity have not been detected in NB.</p> <p>Conclusion</p> <p>Because downregulation of other complexes of the oxidative phosphorylation system was also observed, a more generalized reduction of mitochondrial respiration seems to be present in neuroblastoma in contrast to the single enzyme defect found in hereditary pheochromocytomas.</p> |
| first_indexed | 2024-12-11T20:42:19Z |
| format | Article |
| id | doaj.art-be1f8fdf6a4a444bb5b660412c7f8996 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T20:42:19Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-be1f8fdf6a4a444bb5b660412c7f89962022-12-22T00:51:29ZengBMCBMC Cancer1471-24072010-04-0110114910.1186/1471-2407-10-149Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastomaFeichtinger Rene' GZimmermann FranzMayr Johannes ANeureiter DanielHauser-Kronberger CorneliaSchilling Freimut HJones NeilSperl WolfgangKofler Barbara<p>Abstract</p> <p>Background</p> <p>Succinate dehydrogenase (SDH) has been associated with carcinogenesis in pheochromocytoma and paraganglioma. In the present study we investigated components of the oxidative phosphorylation system in human neuroblastoma tissue samples.</p> <p>Methods</p> <p>Spectrophotometric measurements, immunohistochemical analysis and Western blot analysis were used to characterize the aerobic mitochondrial energy metabolism in neuroblastomas (NB).</p> <p>Results</p> <p>Compared to mitochondrial citrate synthase, SDH activity was severely reduced in NB (n = 14) versus kidney tissue. However no pathogenic mutations could be identified in any of the four subunits of SDH. Furthermore, no genetic alterations could be identified in the two novel SDH assembly factors SDHAF1 and SDH5. Alterations in genes encoding nfs-1, frataxin and isd-11 that could lead to a diminished SDH activity have not been detected in NB.</p> <p>Conclusion</p> <p>Because downregulation of other complexes of the oxidative phosphorylation system was also observed, a more generalized reduction of mitochondrial respiration seems to be present in neuroblastoma in contrast to the single enzyme defect found in hereditary pheochromocytomas.</p>http://www.biomedcentral.com/1471-2407/10/149 |
| spellingShingle | Feichtinger Rene' G Zimmermann Franz Mayr Johannes A Neureiter Daniel Hauser-Kronberger Cornelia Schilling Freimut H Jones Neil Sperl Wolfgang Kofler Barbara Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma BMC Cancer |
| title | Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma |
| title_full | Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma |
| title_fullStr | Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma |
| title_full_unstemmed | Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma |
| title_short | Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma |
| title_sort | low aerobic mitochondrial energy metabolism in poorly or undifferentiated neuroblastoma |
| url | http://www.biomedcentral.com/1471-2407/10/149 |
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