DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae)
The <i>Squalus</i> genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity o...
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MDPI AG
2022-07-01
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| author | Ailton A. Ariza Aisni M. C. L. Adachi Pollyana Roque Fabio H. V. Hazin Marcelo Vianna Matheus M. Rotundo Sergio M. Delpiani Juan M. Díaz de Astarloa Gabriela Delpiani Claudio Oliveira Fausto Foresti Vanessa P. Cruz |
| author_facet | Ailton A. Ariza Aisni M. C. L. Adachi Pollyana Roque Fabio H. V. Hazin Marcelo Vianna Matheus M. Rotundo Sergio M. Delpiani Juan M. Díaz de Astarloa Gabriela Delpiani Claudio Oliveira Fausto Foresti Vanessa P. Cruz |
| author_sort | Ailton A. Ariza |
| collection | DOAJ |
| description | The <i>Squalus</i> genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity of molecular data, the present study aimed to identify <i>Squalus</i> genus MOTUs, using DNA barcoding for species delimitation via ABGD (automatic barcode gap discovery), PTP (Poisson tree process), and GMYC (general mixed Yule coalescent) employing the mitochondrial COI gene. A total of 69 sequences were generated from samples obtained from the American coast in both the Atlantic and Pacific Oceans. The ABGD analysis was the most conservative among the three applied delimitations, indicating three taxonomic units, while the PTP analysis revealed nine MOTUs, with two conflicting units noted between <i>S. clarkae</i> + <i>S. mitsukurii</i> and <i>S. albicaudus</i> + <i>S. cubensis</i>. The GMYC analysis indicated an excessive division, with <i>S. acanthias</i> and <i>S. mitsukurii</i> subdivided into six MOTUs each and <i>S. blainville</i>, into four. These findings demonstrated that <i>Squalus</i> presents a complex of previously defined species, with misidentified samples deposited in databases leading to difficulties in analyzing the real distribution and diversity of species belonging to this genus. Thus, further efforts to highlight possible new species are recommended. |
| first_indexed | 2024-03-09T10:20:36Z |
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| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T10:20:36Z |
| publishDate | 2022-07-01 |
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| spelling | doaj.art-be1fc3495d644cd4a3c1aede134086202023-12-01T22:03:48ZengMDPI AGDiversity1424-28182022-07-0114754410.3390/d14070544DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae)Ailton A. Ariza0Aisni M. C. L. Adachi1Pollyana Roque2Fabio H. V. Hazin3Marcelo Vianna4Matheus M. Rotundo5Sergio M. Delpiani6Juan M. Díaz de Astarloa7Gabriela Delpiani8Claudio Oliveira9Fausto Foresti10Vanessa P. Cruz11Laboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu 18618-689, BrazilLaboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu 18618-689, BrazilLaboratório de Oceanografia Pesqueira, Universidade Federal Rural de Pernambuco, Recife 52171-900, BrazilLaboratório de Oceanografia Pesqueira, Universidade Federal Rural de Pernambuco, Recife 52171-900, BrazilLaboratório de Biologia e Tecnologia Pesqueira, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, BrazilAcervo Zoológico da Universidade Santa Cecília, Universidade Santa Cecília, Santos 11045-907, BrazilGrupo de Biotaxonomía Morfológica y Molecular de Peces, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras, Funes 3250, Mar del Plata 7600, ArgentinaGrupo de Biotaxonomía Morfológica y Molecular de Peces, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras, Funes 3250, Mar del Plata 7600, ArgentinaGrupo de Biotaxonomía Morfológica y Molecular de Peces, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras, Funes 3250, Mar del Plata 7600, ArgentinaLaboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu 18618-689, BrazilLaboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu 18618-689, BrazilLaboratório de Biologia e Genética de Peixes, Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu 18618-689, BrazilThe <i>Squalus</i> genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity of molecular data, the present study aimed to identify <i>Squalus</i> genus MOTUs, using DNA barcoding for species delimitation via ABGD (automatic barcode gap discovery), PTP (Poisson tree process), and GMYC (general mixed Yule coalescent) employing the mitochondrial COI gene. A total of 69 sequences were generated from samples obtained from the American coast in both the Atlantic and Pacific Oceans. The ABGD analysis was the most conservative among the three applied delimitations, indicating three taxonomic units, while the PTP analysis revealed nine MOTUs, with two conflicting units noted between <i>S. clarkae</i> + <i>S. mitsukurii</i> and <i>S. albicaudus</i> + <i>S. cubensis</i>. The GMYC analysis indicated an excessive division, with <i>S. acanthias</i> and <i>S. mitsukurii</i> subdivided into six MOTUs each and <i>S. blainville</i>, into four. These findings demonstrated that <i>Squalus</i> presents a complex of previously defined species, with misidentified samples deposited in databases leading to difficulties in analyzing the real distribution and diversity of species belonging to this genus. Thus, further efforts to highlight possible new species are recommended.https://www.mdpi.com/1424-2818/14/7/544dogsharksElasmobranchiimitochondrial DNACOIspecies identification |
| spellingShingle | Ailton A. Ariza Aisni M. C. L. Adachi Pollyana Roque Fabio H. V. Hazin Marcelo Vianna Matheus M. Rotundo Sergio M. Delpiani Juan M. Díaz de Astarloa Gabriela Delpiani Claudio Oliveira Fausto Foresti Vanessa P. Cruz DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae) Diversity dogsharks Elasmobranchii mitochondrial DNA COI species identification |
| title | DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae) |
| title_full | DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae) |
| title_fullStr | DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae) |
| title_full_unstemmed | DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae) |
| title_short | DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the <i>Squalus</i> Genus (Squaliformes: Squalidae) |
| title_sort | dna barcoding and species delimitation for dogfish sharks belonging to the i squalus i genus squaliformes squalidae |
| topic | dogsharks Elasmobranchii mitochondrial DNA COI species identification |
| url | https://www.mdpi.com/1424-2818/14/7/544 |
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