ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION
To study the bioenergy indicators of leykokontsentrate cells after cryopreservation and the possibility of their recovery after incubation in a medium with a low-molecular fraction from cow cord blood were the aim of research. Leykokontsentrate was obtained from donor blood by sedimentation; cryopre...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-12-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/stories/6_2015/gulevsky_6_2015.pdf |
| _version_ | 1797727952631234560 |
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| author | A. K. Yu. S. А. А. I. V. |
| author_facet | A. K. Yu. S. А. А. I. V. |
| author_sort | A. K. |
| collection | DOAJ |
| description | To study the bioenergy indicators of leykokontsentrate cells after cryopreservation and the possibility of their recovery after incubation in a medium with a low-molecular fraction from cow cord blood were the aim of research. Leykokontsentrate was obtained from donor blood by sedimentation; cryopreservation was carried in the slow freezing regimen with 5% dimethylacetamide; amount of ATP, ADP and AMP was determined by chemiluminescence; glycogen — by cytochemical method. Evidence of energy disbalance of leukoconcentrate cells after cryopreservation was obtained. It was shown that the cord blood low-molecular fraction (0.15 mg/ml) activated glycogenolysis and increased contents of ATP, ADP and AMP in leukoconcentrate cells after cryopreservation. It was also shown that the cord blood low-molecular fraction contained energy substrates and metabolites, hormones and macro- and micronutrients. Use of the low-molecular fraction (below 5 kDa) from cord blood as a component of rehabilitating medium for frozen-thawed leukoconcentrate cells contributed to improvement of their energy status, which was manifested as augmentation in the total adenylic pool and glycogenolysis activation. |
| first_indexed | 2024-03-12T11:06:46Z |
| format | Article |
| id | doaj.art-be250bd2a5c54cb7ae4b53a593e3f4c7 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T11:06:46Z |
| publishDate | 2015-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-be250bd2a5c54cb7ae4b53a593e3f4c72023-09-02T03:42:11ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2015-12-0186637910.15407/biotech8.06.063ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTIONA. K. 0Yu. S. 1А. А. 2 I. V. 3GulevskyAkhatovaА. А. SysoevSysoevaTo study the bioenergy indicators of leykokontsentrate cells after cryopreservation and the possibility of their recovery after incubation in a medium with a low-molecular fraction from cow cord blood were the aim of research. Leykokontsentrate was obtained from donor blood by sedimentation; cryopreservation was carried in the slow freezing regimen with 5% dimethylacetamide; amount of ATP, ADP and AMP was determined by chemiluminescence; glycogen — by cytochemical method. Evidence of energy disbalance of leukoconcentrate cells after cryopreservation was obtained. It was shown that the cord blood low-molecular fraction (0.15 mg/ml) activated glycogenolysis and increased contents of ATP, ADP and AMP in leukoconcentrate cells after cryopreservation. It was also shown that the cord blood low-molecular fraction contained energy substrates and metabolites, hormones and macro- and micronutrients. Use of the low-molecular fraction (below 5 kDa) from cord blood as a component of rehabilitating medium for frozen-thawed leukoconcentrate cells contributed to improvement of their energy status, which was manifested as augmentation in the total adenylic pool and glycogenolysis activation.http://biotechnology.kiev.ua/images/stories/6_2015/gulevsky_6_2015.pdfleykokontsentratcryopreservationenergy metabolismcord blood. |
| spellingShingle | A. K. Yu. S. А. А. I. V. ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION Biotechnologia Acta leykokontsentrat cryopreservation energy metabolism cord blood. |
| title | ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION |
| title_full | ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION |
| title_fullStr | ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION |
| title_full_unstemmed | ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION |
| title_short | ENERGY METABOLISM OF PACKED WHITE CELLS AFTER CRYOPRESERVATION AND REHABILITATION IN A MEDIUM CONTAINING A CORD BLOOD LOW-MOLECULAR FRACTION |
| title_sort | energy metabolism of packed white cells after cryopreservation and rehabilitation in a medium containing a cord blood low molecular fraction |
| topic | leykokontsentrat cryopreservation energy metabolism cord blood. |
| url | http://biotechnology.kiev.ua/images/stories/6_2015/gulevsky_6_2015.pdf |
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