A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis.
We have previously illustrated the nitrate/nitrite respiratory pathway of Shewanella oneidensis, which is renowned for its remarkable versatility in respiration. Here we investigated the systems regulating the pathway with a reliable approach which enables characterization of mutants impaired in nit...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3519889?pdf=render |
| _version_ | 1818267476084916224 |
|---|---|
| author | Yangyang Dong Jixuan Wang Huihui Fu Guangqi Zhou Miaomiao Shi Haichun Gao |
| author_facet | Yangyang Dong Jixuan Wang Huihui Fu Guangqi Zhou Miaomiao Shi Haichun Gao |
| author_sort | Yangyang Dong |
| collection | DOAJ |
| description | We have previously illustrated the nitrate/nitrite respiratory pathway of Shewanella oneidensis, which is renowned for its remarkable versatility in respiration. Here we investigated the systems regulating the pathway with a reliable approach which enables characterization of mutants impaired in nitrate/nitrite respiration by guaranteeing biomass. The S. oneidensis genome encodes an Escherichia coli NarQ/NarX homolog SO3981 and two E. coli NarP/NarL homologs SO1860 and SO3982. Results of physiological characterization and mutational analyses demonstrated that S. oneidensis possesses a single two-component system (TCS) for regulation of nitrate/nitrite respiration, consisting of the sensor kinase SO3981(NarQ) and the response regulator SO3982(NarP). The TCS directly controls the transcription of nap and nrfA (genes encoding nitrate and nitrite reductases, respectively) but regulates the former less tightly than the latter. Additionally, phosphorylation at residue 57 of SO3982 is essential for its DNA-binding capacity. At the global control level, Crp is found to regulate expression of narQP as well as nap and nrfA. In contrast to NarP-NarQ, Crp is more essential for nap rather than nrfA. |
| first_indexed | 2024-12-12T20:23:12Z |
| format | Article |
| id | doaj.art-be294e8f09954f76bbbf77b6a1fa5e76 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T20:23:12Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-be294e8f09954f76bbbf77b6a1fa5e762022-12-22T00:13:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5164310.1371/journal.pone.0051643A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis.Yangyang DongJixuan WangHuihui FuGuangqi ZhouMiaomiao ShiHaichun GaoWe have previously illustrated the nitrate/nitrite respiratory pathway of Shewanella oneidensis, which is renowned for its remarkable versatility in respiration. Here we investigated the systems regulating the pathway with a reliable approach which enables characterization of mutants impaired in nitrate/nitrite respiration by guaranteeing biomass. The S. oneidensis genome encodes an Escherichia coli NarQ/NarX homolog SO3981 and two E. coli NarP/NarL homologs SO1860 and SO3982. Results of physiological characterization and mutational analyses demonstrated that S. oneidensis possesses a single two-component system (TCS) for regulation of nitrate/nitrite respiration, consisting of the sensor kinase SO3981(NarQ) and the response regulator SO3982(NarP). The TCS directly controls the transcription of nap and nrfA (genes encoding nitrate and nitrite reductases, respectively) but regulates the former less tightly than the latter. Additionally, phosphorylation at residue 57 of SO3982 is essential for its DNA-binding capacity. At the global control level, Crp is found to regulate expression of narQP as well as nap and nrfA. In contrast to NarP-NarQ, Crp is more essential for nap rather than nrfA.http://europepmc.org/articles/PMC3519889?pdf=render |
| spellingShingle | Yangyang Dong Jixuan Wang Huihui Fu Guangqi Zhou Miaomiao Shi Haichun Gao A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis. PLoS ONE |
| title | A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis. |
| title_full | A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis. |
| title_fullStr | A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis. |
| title_full_unstemmed | A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis. |
| title_short | A Crp-dependent two-component system regulates nitrate and nitrite respiration in Shewanella oneidensis. |
| title_sort | crp dependent two component system regulates nitrate and nitrite respiration in shewanella oneidensis |
| url | http://europepmc.org/articles/PMC3519889?pdf=render |
| work_keys_str_mv | AT yangyangdong acrpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT jixuanwang acrpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT huihuifu acrpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT guangqizhou acrpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT miaomiaoshi acrpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT haichungao acrpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT yangyangdong crpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT jixuanwang crpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT huihuifu crpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT guangqizhou crpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT miaomiaoshi crpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis AT haichungao crpdependenttwocomponentsystemregulatesnitrateandnitriterespirationinshewanellaoneidensis |