DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods
Cell quantification is widely used both in basic and applied research. A typical example of its use is drug discovery research. Presently, plenty of methods for cell quantification are available. In this review, the basic techniques used for cell quantification, with a special emphasis on techniques...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/18/5515 |
| _version_ | 1797518030668824576 |
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| author | Anna Ligasová Karel Koberna |
| author_facet | Anna Ligasová Karel Koberna |
| author_sort | Anna Ligasová |
| collection | DOAJ |
| description | Cell quantification is widely used both in basic and applied research. A typical example of its use is drug discovery research. Presently, plenty of methods for cell quantification are available. In this review, the basic techniques used for cell quantification, with a special emphasis on techniques based on fluorescent DNA dyes, are described. The main aim of this review is to guide readers through the possibilities of cell quantification with various methods and to show the strengths and weaknesses of these methods, especially with respect to their sensitivity, accuracy, and length. As these methods are frequently accompanied by an analysis of cell proliferation and cell viability, some of these approaches are also described. |
| first_indexed | 2024-03-10T07:24:28Z |
| format | Article |
| id | doaj.art-be2a1890feb34004a4f2ad1369f62f89 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T07:24:28Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-be2a1890feb34004a4f2ad1369f62f892023-11-22T14:24:16ZengMDPI AGMolecules1420-30492021-09-012618551510.3390/molecules26185515DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification MethodsAnna Ligasová0Karel Koberna1Institute of Molecular and Translational Medicine Dentistry, Faculty of Medicine and Dentistry and Czech Advanced Technology Research Institute, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech RepublicInstitute of Molecular and Translational Medicine Dentistry, Faculty of Medicine and Dentistry and Czech Advanced Technology Research Institute, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech RepublicCell quantification is widely used both in basic and applied research. A typical example of its use is drug discovery research. Presently, plenty of methods for cell quantification are available. In this review, the basic techniques used for cell quantification, with a special emphasis on techniques based on fluorescent DNA dyes, are described. The main aim of this review is to guide readers through the possibilities of cell quantification with various methods and to show the strengths and weaknesses of these methods, especially with respect to their sensitivity, accuracy, and length. As these methods are frequently accompanied by an analysis of cell proliferation and cell viability, some of these approaches are also described.https://www.mdpi.com/1420-3049/26/18/5515DNA dyescell quantificationenzymatic conversion of substratecell metabolism |
| spellingShingle | Anna Ligasová Karel Koberna DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods Molecules DNA dyes cell quantification enzymatic conversion of substrate cell metabolism |
| title | DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods |
| title_full | DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods |
| title_fullStr | DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods |
| title_full_unstemmed | DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods |
| title_short | DNA Dyes—Highly Sensitive Reporters of Cell Quantification: Comparison with Other Cell Quantification Methods |
| title_sort | dna dyes highly sensitive reporters of cell quantification comparison with other cell quantification methods |
| topic | DNA dyes cell quantification enzymatic conversion of substrate cell metabolism |
| url | https://www.mdpi.com/1420-3049/26/18/5515 |
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