Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate
Glyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/9/3/104 |
| _version_ | 1819128226372386816 |
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| author | Julia Döring David Rettke Gerhard Rödel Tilo Pompe Kai Ostermann |
| author_facet | Julia Döring David Rettke Gerhard Rödel Tilo Pompe Kai Ostermann |
| author_sort | Julia Döring |
| collection | DOAJ |
| description | Glyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosate usually requires specialized, costly instruments, is labor intensive and time consuming. Here we present a fast and simple method to detect glyphosate in the nanomolar range based on the surface immobilization of glyphosate’s target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) via fusion to the hydrophobin Ccg2 and determination of enzyme activity with a malachite green assay, which is a common photometric technique to measure inorganic phosphate (Pi). The assay demonstrates a new approach for a fast and simple detection of pesticides. |
| first_indexed | 2024-12-22T08:24:27Z |
| format | Article |
| id | doaj.art-be30331c2af348fe9ca36faa8b1f6e1d |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-22T08:24:27Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-be30331c2af348fe9ca36faa8b1f6e1d2022-12-21T18:32:40ZengMDPI AGBiosensors2079-63742019-08-019310410.3390/bios9030104bios9030104Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of GlyphosateJulia Döring0David Rettke1Gerhard Rödel2Tilo Pompe3Kai Ostermann4Institute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, GermanyInstitute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, GermanyInstitute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, GermanyInstitute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, GermanyInstitute of Genetics, Technische Universität Dresden, Zellescher Weg 20b, 01217 Dresden, GermanyGlyphosate, the most widely used pesticide worldwide, is under debate due to its potentially cancerogenic effects and harmful influence on biodiversity and environment. Therefore, the detection of glyphosate in water, food or environmental probes is of high interest. Currently detection of glyphosate usually requires specialized, costly instruments, is labor intensive and time consuming. Here we present a fast and simple method to detect glyphosate in the nanomolar range based on the surface immobilization of glyphosate’s target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) via fusion to the hydrophobin Ccg2 and determination of enzyme activity with a malachite green assay, which is a common photometric technique to measure inorganic phosphate (Pi). The assay demonstrates a new approach for a fast and simple detection of pesticides.https://www.mdpi.com/2079-6374/9/3/104glyphosatemalachite green assayhydrophobinEPSPSimmobilization |
| spellingShingle | Julia Döring David Rettke Gerhard Rödel Tilo Pompe Kai Ostermann Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate Biosensors glyphosate malachite green assay hydrophobin EPSPS immobilization |
| title | Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate |
| title_full | Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate |
| title_fullStr | Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate |
| title_full_unstemmed | Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate |
| title_short | Surface Functionalization by Hydrophobin-EPSPS Fusion Protein Allows for the Fast and Simple Detection of Glyphosate |
| title_sort | surface functionalization by hydrophobin epsps fusion protein allows for the fast and simple detection of glyphosate |
| topic | glyphosate malachite green assay hydrophobin EPSPS immobilization |
| url | https://www.mdpi.com/2079-6374/9/3/104 |
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