Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda
Abstract Human adenoviruses (HAdV) are a diverse group of viruses causing a broad range of infections of the respiratory, urogenital and gastrointestinal tracts and keratoconjunctivitis. There are seven species of human adenoviruses with 113 genotypes which may contain multiple genetic variants. Thi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-06-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-023-08403-9 |
| _version_ | 1797789935907897344 |
|---|---|
| author | Qouilazoni A. Ukuli Bernard Erima Andrew Mubiru Gladys Atim Titus Tugume Hannah Kibuuka Edison Mworozi Mariette F. Ducatez Fred Wabwire-Mangen Denis K. Byarugaba |
| author_facet | Qouilazoni A. Ukuli Bernard Erima Andrew Mubiru Gladys Atim Titus Tugume Hannah Kibuuka Edison Mworozi Mariette F. Ducatez Fred Wabwire-Mangen Denis K. Byarugaba |
| author_sort | Qouilazoni A. Ukuli |
| collection | DOAJ |
| description | Abstract Human adenoviruses (HAdV) are a diverse group of viruses causing a broad range of infections of the respiratory, urogenital and gastrointestinal tracts and keratoconjunctivitis. There are seven species of human adenoviruses with 113 genotypes which may contain multiple genetic variants. This study characterised respiratory human adenoviruses and associated factors in samples collected from selected hospitals in Uganda. A total of 2,298 nasopharyngeal samples were collected between the period of 2008 to 2016 from patients seeking health care at tertiary hospitals for influenza-like illness. They were screened by polymerase chain reaction (PCR) to determine the prevalence of HAdV. HAdV was cultured in A549 cell lines and the hexon gene was sequenced for genotyping. Of the 2,298 samples tested, 225 (9.8%) were adenovirus-positive by PCR. Age was found to be significantly associated with HAdV infections (p = 0.028) with 98% (220/225) of the positives in children aged 5 years and below and none in adults above 25 years of age. The sequenced isolates belonged to species HAdV-B and HAdV-C with most isolates identified as genotype B3. The results showed a high prevalence and genetic diversity in respiratory HAdV circulating in Ugandan population. Deeper genomic characterization based on whole genome sequencing may be necessary to further elucidate possible transmission and impact of current adenovirus-vectored vaccines in Africa. |
| first_indexed | 2024-03-13T01:57:43Z |
| format | Article |
| id | doaj.art-be337b112dd54fdb9018ccea7be1f3b6 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-03-13T01:57:43Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-be337b112dd54fdb9018ccea7be1f3b62023-07-02T11:08:48ZengBMCBMC Infectious Diseases1471-23342023-06-012311910.1186/s12879-023-08403-9Molecular characterisation of human adenoviruses associated with respiratory infections in UgandaQouilazoni A. Ukuli0Bernard Erima1Andrew Mubiru2Gladys Atim3Titus Tugume4Hannah Kibuuka5Edison Mworozi6Mariette F. Ducatez7Fred Wabwire-Mangen8Denis K. Byarugaba9Makerere University Walter Reed ProjectMakerere University Walter Reed ProjectMakerere University Walter Reed ProjectMakerere University Walter Reed ProjectMakerere University Walter Reed ProjectMakerere University Walter Reed ProjectCollege of Health Sciences, Makerere UniversityIHAP, UMR1225, Université de Toulouse, INRAE, ENVTMakerere University Walter Reed ProjectMakerere University Walter Reed ProjectAbstract Human adenoviruses (HAdV) are a diverse group of viruses causing a broad range of infections of the respiratory, urogenital and gastrointestinal tracts and keratoconjunctivitis. There are seven species of human adenoviruses with 113 genotypes which may contain multiple genetic variants. This study characterised respiratory human adenoviruses and associated factors in samples collected from selected hospitals in Uganda. A total of 2,298 nasopharyngeal samples were collected between the period of 2008 to 2016 from patients seeking health care at tertiary hospitals for influenza-like illness. They were screened by polymerase chain reaction (PCR) to determine the prevalence of HAdV. HAdV was cultured in A549 cell lines and the hexon gene was sequenced for genotyping. Of the 2,298 samples tested, 225 (9.8%) were adenovirus-positive by PCR. Age was found to be significantly associated with HAdV infections (p = 0.028) with 98% (220/225) of the positives in children aged 5 years and below and none in adults above 25 years of age. The sequenced isolates belonged to species HAdV-B and HAdV-C with most isolates identified as genotype B3. The results showed a high prevalence and genetic diversity in respiratory HAdV circulating in Ugandan population. Deeper genomic characterization based on whole genome sequencing may be necessary to further elucidate possible transmission and impact of current adenovirus-vectored vaccines in Africa.https://doi.org/10.1186/s12879-023-08403-9Human adenovirusesRespiratory infectionUganda |
| spellingShingle | Qouilazoni A. Ukuli Bernard Erima Andrew Mubiru Gladys Atim Titus Tugume Hannah Kibuuka Edison Mworozi Mariette F. Ducatez Fred Wabwire-Mangen Denis K. Byarugaba Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda BMC Infectious Diseases Human adenoviruses Respiratory infection Uganda |
| title | Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda |
| title_full | Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda |
| title_fullStr | Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda |
| title_full_unstemmed | Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda |
| title_short | Molecular characterisation of human adenoviruses associated with respiratory infections in Uganda |
| title_sort | molecular characterisation of human adenoviruses associated with respiratory infections in uganda |
| topic | Human adenoviruses Respiratory infection Uganda |
| url | https://doi.org/10.1186/s12879-023-08403-9 |
| work_keys_str_mv | AT qouilazoniaukuli molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT bernarderima molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT andrewmubiru molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT gladysatim molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT titustugume molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT hannahkibuuka molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT edisonmworozi molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT mariettefducatez molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT fredwabwiremangen molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda AT deniskbyarugaba molecularcharacterisationofhumanadenovirusesassociatedwithrespiratoryinfectionsinuganda |