Manufacture and extraction of PLLA films with incorporated metal ions
For the in vitro examination of the effects on cells and tissues of metal ions derived from implants by wear and corrosion, a system is needed to release metal ions in a controlled and sustained manner. As small concentrations may have an impact on biological systems, a corresponding slow release ov...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2023-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2023-1113 |
| _version_ | 1797646332469444608 |
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| author | Eickner Thomas Illner Sabine Hahn Olga Müller-Hilke Brigitte Jonitz-Heincke Anika Bader Rainer Fiedler Tomas Peters Kirsten Grabow Niels |
| author_facet | Eickner Thomas Illner Sabine Hahn Olga Müller-Hilke Brigitte Jonitz-Heincke Anika Bader Rainer Fiedler Tomas Peters Kirsten Grabow Niels |
| author_sort | Eickner Thomas |
| collection | DOAJ |
| description | For the in vitro examination of the effects on cells and tissues of metal ions derived from implants by wear and corrosion, a system is needed to release metal ions in a controlled and sustained manner. As small concentrations may have an impact on biological systems, a corresponding slow release over a defined period of time is necessary. Here, a manufacturing method of polymeric Poly(L-lactic acid) (PLLA) films with incorporated cobalt ions via dip coating is reported. The establishment of two analytical methods for the detection and quantification of cobalt ions is shown. High performance liquid chromatography enables cobalt quantification over a concentration range of 0.33 μg/mL up to 33 μg/mL in sample solutions. Extraction and detection of cobalt ions from solvent cast PLLA-cobalt-films shows the feasibility of the manufacturing method. Initial biocompatibility tests with manufactured PLLA-cobaltfilms showed no effects on adipose derived primary mesenchymal stem cells (adMSCs). |
| first_indexed | 2024-03-11T15:01:09Z |
| format | Article |
| id | doaj.art-be34c32b0c774a2a8d389494a9116b8b |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-03-11T15:01:09Z |
| publishDate | 2023-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-be34c32b0c774a2a8d389494a9116b8b2023-10-30T07:58:12ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042023-09-019145145410.1515/cdbme-2023-1113Manufacture and extraction of PLLA films with incorporated metal ionsEickner Thomas0Illner Sabine1Hahn Olga2Müller-Hilke Brigitte3Jonitz-Heincke Anika4Bader Rainer5Fiedler Tomas6Peters Kirsten7Grabow Niels8Institute for Biomedical Engineering, University Medical Center Rostock, Friedrich-Barnewitz-Str. 4, 18119Rostock, GermanyInstitute for Biomedical Engineering, University Medical Center Rostock, Friedrich- Barnewitz-Str. 4, 18119Rostock, GermanyDepartment of Cell Biology, University Medical Center Rostock, Schillingallee 69, 18057Rostock, GermanyInstitute of Immunology, University Medical Center Rostock, Schillingallee 70, 18057Rostock, GermanyDepartment of Orthopedics, University Medical Center Rostock, Doberaner Straße 142, 18057Rostock, GermanyDepartment of Orthopedics, University Medical Center Rostock, Doberaner Straße 142, 18057Rostock, GermanyInstitute for Medical Microbiology, Virology and Hygiene, University Medical Center Rostock, Schillingallee 70, 18057Rostock, GermanyDepartment of Cell Biology, University Medical Center Rostock, Schillingallee 69, 18057Rostock, GermanyInstitute for Biomedical Engineering, University Medical Center Rostock, Friedrich- Barnewitz-Str. 4, 18119Rostock, GermanyFor the in vitro examination of the effects on cells and tissues of metal ions derived from implants by wear and corrosion, a system is needed to release metal ions in a controlled and sustained manner. As small concentrations may have an impact on biological systems, a corresponding slow release over a defined period of time is necessary. Here, a manufacturing method of polymeric Poly(L-lactic acid) (PLLA) films with incorporated cobalt ions via dip coating is reported. The establishment of two analytical methods for the detection and quantification of cobalt ions is shown. High performance liquid chromatography enables cobalt quantification over a concentration range of 0.33 μg/mL up to 33 μg/mL in sample solutions. Extraction and detection of cobalt ions from solvent cast PLLA-cobalt-films shows the feasibility of the manufacturing method. Initial biocompatibility tests with manufactured PLLA-cobaltfilms showed no effects on adipose derived primary mesenchymal stem cells (adMSCs).https://doi.org/10.1515/cdbme-2023-1113metal ionswearcobaltplla filmshplc uv-visadmsc |
| spellingShingle | Eickner Thomas Illner Sabine Hahn Olga Müller-Hilke Brigitte Jonitz-Heincke Anika Bader Rainer Fiedler Tomas Peters Kirsten Grabow Niels Manufacture and extraction of PLLA films with incorporated metal ions Current Directions in Biomedical Engineering metal ions wear cobalt plla films hplc uv-vis admsc |
| title | Manufacture and extraction of PLLA films with incorporated metal ions |
| title_full | Manufacture and extraction of PLLA films with incorporated metal ions |
| title_fullStr | Manufacture and extraction of PLLA films with incorporated metal ions |
| title_full_unstemmed | Manufacture and extraction of PLLA films with incorporated metal ions |
| title_short | Manufacture and extraction of PLLA films with incorporated metal ions |
| title_sort | manufacture and extraction of plla films with incorporated metal ions |
| topic | metal ions wear cobalt plla films hplc uv-vis admsc |
| url | https://doi.org/10.1515/cdbme-2023-1113 |
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