Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub>
Clot retraction results from retractions of platelet filopodia and fibrin fibers and requires the functional platelet α<sub>IIb</sub>β<sub>3</sub> integrin. This assay is widely used to test the functions of platelets and fibrinogen as well as the efficacy of fibrinolysis. Ch...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/9/2345 |
| _version_ | 1797581189013307392 |
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| author | Daniel Gao Caroline W. Sun Angela B. Woodley Jing-fei Dong |
| author_facet | Daniel Gao Caroline W. Sun Angela B. Woodley Jing-fei Dong |
| author_sort | Daniel Gao |
| collection | DOAJ |
| description | Clot retraction results from retractions of platelet filopodia and fibrin fibers and requires the functional platelet α<sub>IIb</sub>β<sub>3</sub> integrin. This assay is widely used to test the functions of platelets and fibrinogen as well as the efficacy of fibrinolysis. Changes in clot retraction have been found in a variety of hemostatic abnormalities and, more recently, in arterial thrombosis. Despite its broad clinical use and low cost, many aspects of clot retraction are poorly understood. In the present study, we performed two clinical standard clot retraction assays using whole-blood and platelet-rich plasma (PRP) samples to determine how clot retraction correlates with platelet counts and mean volume, the density of α<sub>IIb</sub>β<sub>3</sub> integrin and PLA genotypes, and plasma fibrinogen levels. We found that clot retraction was affected by platelet counts, but not mean platelet volume. It correlated with the surface density of the integrin α<sub>Iib</sub>β<sub>3</sub>, but not PLA genotypes. These results indicate that clot retraction measures a unique aspect of platelet function and can serve as an additional means to detect functional changes in platelets. |
| first_indexed | 2024-03-10T23:01:47Z |
| format | Article |
| id | doaj.art-be3635023bf64597a3b2fd70f3c6eeb3 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T23:01:47Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-be3635023bf64597a3b2fd70f3c6eeb32023-11-19T09:39:59ZengMDPI AGBiomedicines2227-90592023-08-01119234510.3390/biomedicines11092345Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub>Daniel Gao0Caroline W. Sun1Angela B. Woodley2Jing-fei Dong3Bloodworks Research Institute, 1551 Southlake Ave. E., Seattle, WA 98102, USASection of Cardiovascular Sciences, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USASection of Cardiovascular Sciences, Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USABloodworks Research Institute, 1551 Southlake Ave. E., Seattle, WA 98102, USAClot retraction results from retractions of platelet filopodia and fibrin fibers and requires the functional platelet α<sub>IIb</sub>β<sub>3</sub> integrin. This assay is widely used to test the functions of platelets and fibrinogen as well as the efficacy of fibrinolysis. Changes in clot retraction have been found in a variety of hemostatic abnormalities and, more recently, in arterial thrombosis. Despite its broad clinical use and low cost, many aspects of clot retraction are poorly understood. In the present study, we performed two clinical standard clot retraction assays using whole-blood and platelet-rich plasma (PRP) samples to determine how clot retraction correlates with platelet counts and mean volume, the density of α<sub>IIb</sub>β<sub>3</sub> integrin and PLA genotypes, and plasma fibrinogen levels. We found that clot retraction was affected by platelet counts, but not mean platelet volume. It correlated with the surface density of the integrin α<sub>Iib</sub>β<sub>3</sub>, but not PLA genotypes. These results indicate that clot retraction measures a unique aspect of platelet function and can serve as an additional means to detect functional changes in platelets.https://www.mdpi.com/2227-9059/11/9/2345clot retractionintegrin α<sub>Iib</sub>β<sub>3</sub>PLA genotypes |
| spellingShingle | Daniel Gao Caroline W. Sun Angela B. Woodley Jing-fei Dong Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub> Biomedicines clot retraction integrin α<sub>Iib</sub>β<sub>3</sub> PLA genotypes |
| title | Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub> |
| title_full | Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub> |
| title_fullStr | Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub> |
| title_full_unstemmed | Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub> |
| title_short | Clot Retraction and Its Correlation with the Function of Platelet Integrin α<sub>IIb</sub>β<sub>3</sub> |
| title_sort | clot retraction and its correlation with the function of platelet integrin α sub iib sub β sub 3 sub |
| topic | clot retraction integrin α<sub>Iib</sub>β<sub>3</sub> PLA genotypes |
| url | https://www.mdpi.com/2227-9059/11/9/2345 |
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