First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy
The urine from 35 giraffes was studied by untargeted <sup>1</sup>H-NMR, with the purpose of obtaining, for the first time, a fingerprint of its metabolome. The metabolome, as downstream of the transcriptome and proteome, has been considered as the most representative approach to monitor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/4/157 |
| _version_ | 1797570432458555392 |
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| author | Chenglin Zhu Sabrina Fasoli Gloria Isani Luca Laghi |
| author_facet | Chenglin Zhu Sabrina Fasoli Gloria Isani Luca Laghi |
| author_sort | Chenglin Zhu |
| collection | DOAJ |
| description | The urine from 35 giraffes was studied by untargeted <sup>1</sup>H-NMR, with the purpose of obtaining, for the first time, a fingerprint of its metabolome. The metabolome, as downstream of the transcriptome and proteome, has been considered as the most representative approach to monitor the relationships between animal physiological features and environment. Thirty-nine molecules were unambiguously quantified, able to give information about diet, proteins digestion, energy generation, and gut-microbial co-metabolism. The samples collected allowed study of the effects of age and sex on the giraffe urinary metabolome. In addition, preliminary information about how sampling procedure and pregnancy could affect a giraffe’s urinary metabolome was obtained. Such work could trigger the setting up of methods to non-invasively study the health status of giraffes, which is utterly needed, considering that anesthetic-related complications make their immobilization a very risky practice. |
| first_indexed | 2024-03-10T20:24:29Z |
| format | Article |
| id | doaj.art-be3a62afaa4e4e03bed9f154362a7fd5 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T20:24:29Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-be3a62afaa4e4e03bed9f154362a7fd52023-11-19T21:55:57ZengMDPI AGMetabolites2218-19892020-04-0110415710.3390/metabo10040157First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance SpectroscopyChenglin Zhu0Sabrina Fasoli1Gloria Isani2Luca Laghi3Department of Agro-Food Science and Technology, University of Bologna, 47521 Cesena, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, 40064 Bologna, ItalyDepartment of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, 40064 Bologna, ItalyDepartment of Agro-Food Science and Technology, University of Bologna, 47521 Cesena, ItalyThe urine from 35 giraffes was studied by untargeted <sup>1</sup>H-NMR, with the purpose of obtaining, for the first time, a fingerprint of its metabolome. The metabolome, as downstream of the transcriptome and proteome, has been considered as the most representative approach to monitor the relationships between animal physiological features and environment. Thirty-nine molecules were unambiguously quantified, able to give information about diet, proteins digestion, energy generation, and gut-microbial co-metabolism. The samples collected allowed study of the effects of age and sex on the giraffe urinary metabolome. In addition, preliminary information about how sampling procedure and pregnancy could affect a giraffe’s urinary metabolome was obtained. Such work could trigger the setting up of methods to non-invasively study the health status of giraffes, which is utterly needed, considering that anesthetic-related complications make their immobilization a very risky practice.https://www.mdpi.com/2218-1989/10/4/157captive giraffesurinemetabolomics<sup>1</sup>H-NMR |
| spellingShingle | Chenglin Zhu Sabrina Fasoli Gloria Isani Luca Laghi First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy Metabolites captive giraffes urine metabolomics <sup>1</sup>H-NMR |
| title | First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy |
| title_full | First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy |
| title_fullStr | First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy |
| title_full_unstemmed | First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy |
| title_short | First Insights into the Urinary Metabolome of Captive Giraffes by Proton Nuclear Magnetic Resonance Spectroscopy |
| title_sort | first insights into the urinary metabolome of captive giraffes by proton nuclear magnetic resonance spectroscopy |
| topic | captive giraffes urine metabolomics <sup>1</sup>H-NMR |
| url | https://www.mdpi.com/2218-1989/10/4/157 |
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