Isolation of Keratin from Waste Wool Using Hydrothermal Processes
The subcritical water (SubCW) extractions of waste wool to produce keratin were performed at temperatures of 150 °C to 250 °C and at different reaction times between 5 min to 75 min. The resulting proteins in the obtained products were confirmed with Fourier-transform infrared spectroscopy (FTIR). T...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Slovenian Chemical Society
2021-06-01
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| Series: | Acta Chimica Slovenica |
| Subjects: | |
| Online Access: | https://journals.matheo.si/index.php/ACSi/article/view/6538 |
| _version_ | 1818887248901308416 |
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| author | Aleksandra Verdnik Maja Čolnik Željko Knez Mojca Škerget |
| author_facet | Aleksandra Verdnik Maja Čolnik Željko Knez Mojca Škerget |
| author_sort | Aleksandra Verdnik |
| collection | DOAJ |
| description | The subcritical water (SubCW) extractions of waste wool to produce keratin were performed at temperatures of 150 °C to 250 °C and at different reaction times between 5 min to 75 min. The resulting proteins in the obtained products were confirmed with Fourier-transform infrared spectroscopy (FTIR). The molecular weight of the protein extracts was determined by using two different methods: with a polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE) and by using a gel permeation chromatography. The results show, that by using SubCW, keratin can be isolated from waste wool in very high yields, much higher than by other chemical methods. Maximal yield was achieved at 180 °C and 60 min and it was 90.3%. The molecular weight distributions of extracted proteins, which were generated from waste wool were between 14 kDa and 4 kDa, what is comparable to the results obtained by other chemical methods. |
| first_indexed | 2024-12-19T16:34:13Z |
| format | Article |
| id | doaj.art-be3ac68cf1fe4d33bb58010aa6acff1c |
| institution | Directory Open Access Journal |
| issn | 1318-0207 1580-3155 |
| language | English |
| last_indexed | 2024-12-19T16:34:13Z |
| publishDate | 2021-06-01 |
| publisher | Slovenian Chemical Society |
| record_format | Article |
| series | Acta Chimica Slovenica |
| spelling | doaj.art-be3ac68cf1fe4d33bb58010aa6acff1c2022-12-21T20:14:03ZengSlovenian Chemical SocietyActa Chimica Slovenica1318-02071580-31552021-06-0168243344010.17344/acsi.2020.6538966Isolation of Keratin from Waste Wool Using Hydrothermal ProcessesAleksandra Verdnik0Maja Čolnik1Željko Knez2Mojca Škerget3University of Maribor Faculty of Chemistry and Chemical EngineeringUniversity of Maribor Faculty of Chemistry and Chemical EngineeringUniversity of Maribor Faculty of Chemistry and Chemical EngineeringUniversity of Maribor Faculty of Chemistry and Chemical EngineeringThe subcritical water (SubCW) extractions of waste wool to produce keratin were performed at temperatures of 150 °C to 250 °C and at different reaction times between 5 min to 75 min. The resulting proteins in the obtained products were confirmed with Fourier-transform infrared spectroscopy (FTIR). The molecular weight of the protein extracts was determined by using two different methods: with a polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE) and by using a gel permeation chromatography. The results show, that by using SubCW, keratin can be isolated from waste wool in very high yields, much higher than by other chemical methods. Maximal yield was achieved at 180 °C and 60 min and it was 90.3%. The molecular weight distributions of extracted proteins, which were generated from waste wool were between 14 kDa and 4 kDa, what is comparable to the results obtained by other chemical methods.https://journals.matheo.si/index.php/ACSi/article/view/6538waste woolkeratinisolationsubcwgel permeation chromatographysds-page electrophoresis |
| spellingShingle | Aleksandra Verdnik Maja Čolnik Željko Knez Mojca Škerget Isolation of Keratin from Waste Wool Using Hydrothermal Processes Acta Chimica Slovenica waste wool keratin isolation subcw gel permeation chromatography sds-page electrophoresis |
| title | Isolation of Keratin from Waste Wool Using Hydrothermal Processes |
| title_full | Isolation of Keratin from Waste Wool Using Hydrothermal Processes |
| title_fullStr | Isolation of Keratin from Waste Wool Using Hydrothermal Processes |
| title_full_unstemmed | Isolation of Keratin from Waste Wool Using Hydrothermal Processes |
| title_short | Isolation of Keratin from Waste Wool Using Hydrothermal Processes |
| title_sort | isolation of keratin from waste wool using hydrothermal processes |
| topic | waste wool keratin isolation subcw gel permeation chromatography sds-page electrophoresis |
| url | https://journals.matheo.si/index.php/ACSi/article/view/6538 |
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