Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy
Osteoarthritis (OA) is a common orthopedic chronic disease, and the use of nanomaterials as carriers is an effective way to realize slow and controlled release therapy for OA drugs. In this study, we synthesized a polyethylene glycol-stabilized bilayer-decorated cationic liposome (CLis) as a drug de...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2024-02-01
|
| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/5.0189591 |
| _version_ | 1797278307797958656 |
|---|---|
| author | Xiaodong Liu Hongbin Dai Zhiqing Wang Chunyan Huang Kai Huang |
| author_facet | Xiaodong Liu Hongbin Dai Zhiqing Wang Chunyan Huang Kai Huang |
| author_sort | Xiaodong Liu |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a common orthopedic chronic disease, and the use of nanomaterials as carriers is an effective way to realize slow and controlled release therapy for OA drugs. In this study, we synthesized a polyethylene glycol-stabilized bilayer-decorated cationic liposome (CLis) as a drug delivery system for delivering glucosamine sulfate (GS) drug to achieve the treatment of OA in rats. The CLis encapsulated with GS drug (GS-CLis) was prepared by a reverse evaporation method, and its physical properties, encapsulation rate, and drug release performance were evaluated. The biological properties of GS-CLis were evaluated in vitro. The therapeutic effect of GS-CLis on osteoarthritis was evaluated in vivo, and the in vivo biosafety of the drug was assessed by hematology, blood biochemistry, and hematoxylin-eosin staining. GS-CLis had a particle size of (236.28 ± 4.76) nm, a potential of (27.35 ± 4.86) mv, and a spherical shape. The encapsulation rate was (96.18 ± 5.77)% and the drug-loading capacity was (9.61 ± 0.28)%, which provided a good slow drug release. GS-CLis has low cytotoxicity, low hemolysis rate, and good biocompatibility. GS-CLis can more effectively alleviate joint surface damage and inhibit the expression of inflammatory factors, and has no significant effect on the body weight of rats, with good biological safety. In this study, we successfully synthesized novel GS-CLis for the treatment of OA, which improved the retention time and therapeutic effect of GS in OA and provided a research basis for its development as an OA nanoformulation. |
| first_indexed | 2024-03-07T16:00:21Z |
| format | Article |
| id | doaj.art-be40ff76bf22453fbf29a2d17b27fb30 |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-03-07T16:00:21Z |
| publishDate | 2024-02-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-be40ff76bf22453fbf29a2d17b27fb302024-03-04T21:29:33ZengAIP Publishing LLCAIP Advances2158-32262024-02-01142025137025137-1210.1063/5.0189591Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapyXiaodong Liu0Hongbin Dai1Zhiqing Wang2Chunyan Huang3Kai Huang4Department of Orthopaedics 2, Shanghai Jing’an District Zhabei Central Hospital, 200073 Shanghai, ChinaDepartment of Orthopaedics 2, Shanghai Jing’an District Zhabei Central Hospital, 200073 Shanghai, ChinaDepartment of Orthopaedics 2, Shanghai Jing’an District Zhabei Central Hospital, 200073 Shanghai, ChinaDepartment of Anaesthesia, Shanghai Jing’an District Zhabei Central Hospital, 200073 Shanghai, ChinaDepartment of Orthopaedics 2, Shanghai Jing’an District Zhabei Central Hospital, 200073 Shanghai, ChinaOsteoarthritis (OA) is a common orthopedic chronic disease, and the use of nanomaterials as carriers is an effective way to realize slow and controlled release therapy for OA drugs. In this study, we synthesized a polyethylene glycol-stabilized bilayer-decorated cationic liposome (CLis) as a drug delivery system for delivering glucosamine sulfate (GS) drug to achieve the treatment of OA in rats. The CLis encapsulated with GS drug (GS-CLis) was prepared by a reverse evaporation method, and its physical properties, encapsulation rate, and drug release performance were evaluated. The biological properties of GS-CLis were evaluated in vitro. The therapeutic effect of GS-CLis on osteoarthritis was evaluated in vivo, and the in vivo biosafety of the drug was assessed by hematology, blood biochemistry, and hematoxylin-eosin staining. GS-CLis had a particle size of (236.28 ± 4.76) nm, a potential of (27.35 ± 4.86) mv, and a spherical shape. The encapsulation rate was (96.18 ± 5.77)% and the drug-loading capacity was (9.61 ± 0.28)%, which provided a good slow drug release. GS-CLis has low cytotoxicity, low hemolysis rate, and good biocompatibility. GS-CLis can more effectively alleviate joint surface damage and inhibit the expression of inflammatory factors, and has no significant effect on the body weight of rats, with good biological safety. In this study, we successfully synthesized novel GS-CLis for the treatment of OA, which improved the retention time and therapeutic effect of GS in OA and provided a research basis for its development as an OA nanoformulation.http://dx.doi.org/10.1063/5.0189591 |
| spellingShingle | Xiaodong Liu Hongbin Dai Zhiqing Wang Chunyan Huang Kai Huang Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy AIP Advances |
| title | Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy |
| title_full | Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy |
| title_fullStr | Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy |
| title_full_unstemmed | Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy |
| title_short | Polyethylene glycol-stabilized cationic liposome encapsulating glucosamine sulfate: A promising nanoformulation for osteoarthritis therapy |
| title_sort | polyethylene glycol stabilized cationic liposome encapsulating glucosamine sulfate a promising nanoformulation for osteoarthritis therapy |
| url | http://dx.doi.org/10.1063/5.0189591 |
| work_keys_str_mv | AT xiaodongliu polyethyleneglycolstabilizedcationicliposomeencapsulatingglucosaminesulfateapromisingnanoformulationforosteoarthritistherapy AT hongbindai polyethyleneglycolstabilizedcationicliposomeencapsulatingglucosaminesulfateapromisingnanoformulationforosteoarthritistherapy AT zhiqingwang polyethyleneglycolstabilizedcationicliposomeencapsulatingglucosaminesulfateapromisingnanoformulationforosteoarthritistherapy AT chunyanhuang polyethyleneglycolstabilizedcationicliposomeencapsulatingglucosaminesulfateapromisingnanoformulationforosteoarthritistherapy AT kaihuang polyethyleneglycolstabilizedcationicliposomeencapsulatingglucosaminesulfateapromisingnanoformulationforosteoarthritistherapy |