Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia
The glutathione S-transferase (GST) genes encode enzymes that mediate the detoxification of xenobiotics by catalyzing the conjugation of glutathione (GSH) to xenobiotic substrates. The aim of the current study is to investigate the association between GSTT1 and GSTM1 polymorphisms and chronic myelo...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2019-04-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4176 |
| _version_ | 1797260908792119296 |
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| author | Abdel Rahim Mahmoud Muddathir Elharam Ibrahim Abdallah Omar Falah Khabour Ream Elzain Abdelgader Mahmoud Mohamed Elgari |
| author_facet | Abdel Rahim Mahmoud Muddathir Elharam Ibrahim Abdallah Omar Falah Khabour Ream Elzain Abdelgader Mahmoud Mohamed Elgari |
| author_sort | Abdel Rahim Mahmoud Muddathir |
| collection | DOAJ |
| description |
The glutathione S-transferase (GST) genes encode enzymes that mediate the detoxification of xenobiotics by catalyzing the conjugation of glutathione (GSH) to xenobiotic substrates. The aim of the current study is to investigate the association between GSTT1 and GSTM1 polymorphisms and chronic myeloid leukemia (CML) among Sudanese patients. Patients with CML (n = 115) were recruited to the study from the Radiation and Isotope Centre Khartoum (RICK)-Sudan. Healthy individuals (n = 104) were included as controls. Genotyping of GSTT1 and GSTM1 polymorphisms was performed using multiplex PCR. Null deletions in the GSTT1 and GSTM1 genes are common in the Sudanese population (control group), with frequencies of 33.9% and 38.2%, respectively. The frequencies of GSTT1 (OR: 3.25, 95% CI: 1.87–5.65, p < 0.001) and GSTM1 (OR: 2.14, 95% CI: 1.25–3.67, p < 0.005) null genotypes were significantly higher in CML patients vs. controls. The distribution of GSTT1 and GSTM1 null polymorphisms was not different between male and female (p > 0.01) and young and old CML patients (p > 0.05). Hematological parameters were not affected by null polymorphisms in the patient group (p > 0.05). In addition, the frequency of GSTM1 null polymorphism was lower in advanced-phase CML patients compared to chronic-phase patients (p < 0.05). The GSTT1 and GSTM1 null polymorphisms are associated with CML among Sudanese patients, independently of their age and gender.
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| first_indexed | 2024-04-24T23:32:48Z |
| format | Article |
| id | doaj.art-be430c85f3354432b2d551c239b770e2 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:48Z |
| publishDate | 2019-04-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-be430c85f3354432b2d551c239b770e22024-03-15T14:30:54ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2019-04-0119410.17305/bjbms.2019.4176Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemiaAbdel Rahim Mahmoud Muddathir0Elharam Ibrahim Abdallah1Omar Falah Khabour2https://orcid.org/0000-0002-3006-3104Ream Elzain Abdelgader3https://orcid.org/0000-0001-7507-4774Mahmoud Mohamed Elgari4Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Taibah University, Medina, Saudi Arabia; Department of Hematology and Blood Transfusion, Faculty of Medical Laboratory Sciences, Alzaeim Alazhari University, Khartoum, SudanDepartment of Hematology and Blood Transfusion, Faculty of Medical Laboratory Sciences, Alzaeim Alazhari University, Khartoum, SudanDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Taibah University, Medina, Saudi Arabia; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology, Irbid, JordanDepartment of Hematology and Blood Transfusion, Faculty of Medical Laboratory Sciences, Kordofan University, El-Obeid, SudanDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Taibah University, Medina, Saudi Arabia The glutathione S-transferase (GST) genes encode enzymes that mediate the detoxification of xenobiotics by catalyzing the conjugation of glutathione (GSH) to xenobiotic substrates. The aim of the current study is to investigate the association between GSTT1 and GSTM1 polymorphisms and chronic myeloid leukemia (CML) among Sudanese patients. Patients with CML (n = 115) were recruited to the study from the Radiation and Isotope Centre Khartoum (RICK)-Sudan. Healthy individuals (n = 104) were included as controls. Genotyping of GSTT1 and GSTM1 polymorphisms was performed using multiplex PCR. Null deletions in the GSTT1 and GSTM1 genes are common in the Sudanese population (control group), with frequencies of 33.9% and 38.2%, respectively. The frequencies of GSTT1 (OR: 3.25, 95% CI: 1.87–5.65, p < 0.001) and GSTM1 (OR: 2.14, 95% CI: 1.25–3.67, p < 0.005) null genotypes were significantly higher in CML patients vs. controls. The distribution of GSTT1 and GSTM1 null polymorphisms was not different between male and female (p > 0.01) and young and old CML patients (p > 0.05). Hematological parameters were not affected by null polymorphisms in the patient group (p > 0.05). In addition, the frequency of GSTM1 null polymorphism was lower in advanced-phase CML patients compared to chronic-phase patients (p < 0.05). The GSTT1 and GSTM1 null polymorphisms are associated with CML among Sudanese patients, independently of their age and gender. https://www.bjbms.org/ojs/index.php/bjbms/article/view/4176GSTsglutathione S-transferasenull polymorphismGSTT1GSTM1CML |
| spellingShingle | Abdel Rahim Mahmoud Muddathir Elharam Ibrahim Abdallah Omar Falah Khabour Ream Elzain Abdelgader Mahmoud Mohamed Elgari Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia Biomolecules & Biomedicine GSTs glutathione S-transferase null polymorphism GSTT1 GSTM1 CML |
| title | Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia |
| title_full | Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia |
| title_fullStr | Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia |
| title_full_unstemmed | Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia |
| title_short | Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia |
| title_sort | age and gender independent association of glutathione s transferase null polymorphisms with chronic myeloid leukemia |
| topic | GSTs glutathione S-transferase null polymorphism GSTT1 GSTM1 CML |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4176 |
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