Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology
Bovine leptospirosis causes economic losses and raises public health concerns. It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, such as the Caatinga biome in Brazil, where the climate is hot and dry, and the etiological agent requir...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/8/3/177 |
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| author | Nathanael Natércio da Costa Barnabé Rafael Rodrigues Soares Deivyson Kelvis Silva Barros Denise Batista Nogueira Flávia Teresa Ribeiro da Costa João Pessoa Araújo Júnior Camila Dantas Malossi Leila Sabrina Ullmann Diego Figueiredo da Costa Maria Luana Cristiny Rodrigues Silva Severino Silvano dos Santos Higino Carolina de Sousa Américo Batista Santos Sérgio Santos de Azevedo Clebert José Alves |
| author_facet | Nathanael Natércio da Costa Barnabé Rafael Rodrigues Soares Deivyson Kelvis Silva Barros Denise Batista Nogueira Flávia Teresa Ribeiro da Costa João Pessoa Araújo Júnior Camila Dantas Malossi Leila Sabrina Ullmann Diego Figueiredo da Costa Maria Luana Cristiny Rodrigues Silva Severino Silvano dos Santos Higino Carolina de Sousa Américo Batista Santos Sérgio Santos de Azevedo Clebert José Alves |
| author_sort | Nathanael Natércio da Costa Barnabé |
| collection | DOAJ |
| description | Bovine leptospirosis causes economic losses and raises public health concerns. It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, such as the Caatinga biome in Brazil, where the climate is hot and dry, and the etiological agent require alternative routes of transmission. This study aimed to close knowledge gaps to the diagnosis and epidemiology of <i>Leptospira</i> spp. infection in cows from the Caatinga biome, Brazil. Samples of the blood, urinary tract (urine, bladder and kidney) and reproductive tract (vaginal fluid, uterus, uterine tube, ovary and placenta) were collected from 42 slaughtered cows. Diagnostic tests included were the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-<i>Leptospira</i> spp. antibodies were found in 27 (64.3%) of the animals analyzed using MAT at a 1:50 dilution (cut-off 50), while 31 (73.8%) animals had at least one organ/fluid where the presence of <i>Leptospira</i> spp. DNA was identified, and 29 animals (69%) were positive at bacteriological culture. The highest sensitivity values for MAT were obtained at the cut-off point of 50. In conclusion, even under hot and dry climate conditions, it is possible that <i>Leptospira</i> spp. can spread through alternative routes such as venereal transmission; moreover, a cut-off of 50 is recommended for the serological diagnosis of cattle from the Caatinga biome. |
| first_indexed | 2024-03-11T05:48:31Z |
| format | Article |
| id | doaj.art-be43c64d77b444d49ac4ba67a0fad5a0 |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-11T05:48:31Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-be43c64d77b444d49ac4ba67a0fad5a02023-11-17T14:15:30ZengMDPI AGTropical Medicine and Infectious Disease2414-63662023-03-018317710.3390/tropicalmed8030177Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and EpidemiologyNathanael Natércio da Costa Barnabé0Rafael Rodrigues Soares1Deivyson Kelvis Silva Barros2Denise Batista Nogueira3Flávia Teresa Ribeiro da Costa4João Pessoa Araújo Júnior5Camila Dantas Malossi6Leila Sabrina Ullmann7Diego Figueiredo da Costa8Maria Luana Cristiny Rodrigues Silva9Severino Silvano dos Santos Higino10Carolina de Sousa Américo Batista Santos11Sérgio Santos de Azevedo12Clebert José Alves13Academic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilFaculty of Veterinary Medicine and Zootechny (FMVZ), University of São Paulo (USP), São Paulo 05508-220, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilInstitute of Biosciences, Department of Microbiology and Immunology, University of the São Paulo State (UNESP), Botucatu 18618-687, BrazilInstitute of Biosciences, Department of Microbiology and Immunology, University of the São Paulo State (UNESP), Botucatu 18618-687, BrazilInstitute of Biosciences, Department of Microbiology and Immunology, University of the São Paulo State (UNESP), Botucatu 18618-687, BrazilDepartment of Veterinary Science, Federal University of Paraíba (UFPB), Areia 58397-000, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilAcademic Unit of Veterinary Medicine (UAMV), Federal University of Campina Grande (UFCG), Patos 58708-110, BrazilBovine leptospirosis causes economic losses and raises public health concerns. It is possible that there are peculiarities in the epidemiology of leptospirosis in regions with a semiarid climate, such as the Caatinga biome in Brazil, where the climate is hot and dry, and the etiological agent require alternative routes of transmission. This study aimed to close knowledge gaps to the diagnosis and epidemiology of <i>Leptospira</i> spp. infection in cows from the Caatinga biome, Brazil. Samples of the blood, urinary tract (urine, bladder and kidney) and reproductive tract (vaginal fluid, uterus, uterine tube, ovary and placenta) were collected from 42 slaughtered cows. Diagnostic tests included were the microscopic agglutination test (MAT), polymerase chain reaction (PCR) and bacterial isolation. Anti-<i>Leptospira</i> spp. antibodies were found in 27 (64.3%) of the animals analyzed using MAT at a 1:50 dilution (cut-off 50), while 31 (73.8%) animals had at least one organ/fluid where the presence of <i>Leptospira</i> spp. DNA was identified, and 29 animals (69%) were positive at bacteriological culture. The highest sensitivity values for MAT were obtained at the cut-off point of 50. In conclusion, even under hot and dry climate conditions, it is possible that <i>Leptospira</i> spp. can spread through alternative routes such as venereal transmission; moreover, a cut-off of 50 is recommended for the serological diagnosis of cattle from the Caatinga biome.https://www.mdpi.com/2414-6366/8/3/177<i>Leptospira</i> spp.serologycut-off pointbacteriological culturePCRsemiarid conditions |
| spellingShingle | Nathanael Natércio da Costa Barnabé Rafael Rodrigues Soares Deivyson Kelvis Silva Barros Denise Batista Nogueira Flávia Teresa Ribeiro da Costa João Pessoa Araújo Júnior Camila Dantas Malossi Leila Sabrina Ullmann Diego Figueiredo da Costa Maria Luana Cristiny Rodrigues Silva Severino Silvano dos Santos Higino Carolina de Sousa Américo Batista Santos Sérgio Santos de Azevedo Clebert José Alves Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology Tropical Medicine and Infectious Disease <i>Leptospira</i> spp. serology cut-off point bacteriological culture PCR semiarid conditions |
| title | Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
| title_full | Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
| title_fullStr | Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
| title_full_unstemmed | Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
| title_short | Bovine Leptospirosis in Caatinga Biome, Brazil: New Insights into Diagnosis and Epidemiology |
| title_sort | bovine leptospirosis in caatinga biome brazil new insights into diagnosis and epidemiology |
| topic | <i>Leptospira</i> spp. serology cut-off point bacteriological culture PCR semiarid conditions |
| url | https://www.mdpi.com/2414-6366/8/3/177 |
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