Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy
Objective: Confocal laser scanning microscopy (CLSM) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of CLSM for the biologist is its ability to provide optical sections from three-dimensional specimen. The present...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2009-12-01
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| Series: | Frontiers in Dentistry |
| Subjects: | |
| Online Access: | https://jdt.tums.ac.ir/index.php/jdt/article/view/194 |
| _version_ | 1818472690195890176 |
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| author | KA. Al-Salihi NABA. Tarmidzi |
| author_facet | KA. Al-Salihi NABA. Tarmidzi |
| author_sort | KA. Al-Salihi |
| collection | DOAJ |
| description | Objective:
Confocal laser scanning microscopy (CLSM) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of CLSM for the biologist is its ability to provide optical sections from three-dimensional specimen. The present study was designed to assess the thickness content of in vivo accumulated dental plaque using CLSM and scanning electron microscopy (SEM).
Materials and Methods:
Acroflat lower arch splints (acrylic appliance) were worn five participants for three days without any disturbance. The formed plaques were assessed using CLSM combined with vital fluorescence technique and SEM.
Results:
In this study accumulated dental plaque revealed varied plaque microflora vitality and thickness according to participant's oral hygiene. The thickness of plaque smears ranged from 40.32 to 140.72 µm and 65.00 to 128.88 µm for live (vital) and dead accumulated microorganisms, respectively. Meanwhile, the thickness of plaque on the appliance ranged from 101 µm to 653 µm. CLSM revealed both dead and vital bacteria on the surface of the dental plaque. In addition, SEM revealed layers of various bacterial aggregations in all dental plaques.
Conclusion:
This study offers a potent non-invasive tool to evaluate and assess the dental plaque biofilm, which is a very important factor in the development of dental caries. |
| first_indexed | 2024-04-14T04:11:02Z |
| format | Article |
| id | doaj.art-be480c41b22e41e9821994b5f37e1189 |
| institution | Directory Open Access Journal |
| issn | 2676-296X |
| language | English |
| last_indexed | 2024-04-14T04:11:02Z |
| publishDate | 2009-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Frontiers in Dentistry |
| spelling | doaj.art-be480c41b22e41e9821994b5f37e11892022-12-22T02:13:11ZengTehran University of Medical SciencesFrontiers in Dentistry2676-296X2009-12-0164Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy KA. Al-Salihi 0 NABA. Tarmidzi1Associated Professor, Department of Craniofacial and Oral Sciences, School of Dental Sciences, UniveDental officer, Department of Clinical Studies, School of Dental Sciences, University Sains MalaysiaObjective: Confocal laser scanning microscopy (CLSM) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of CLSM for the biologist is its ability to provide optical sections from three-dimensional specimen. The present study was designed to assess the thickness content of in vivo accumulated dental plaque using CLSM and scanning electron microscopy (SEM). Materials and Methods: Acroflat lower arch splints (acrylic appliance) were worn five participants for three days without any disturbance. The formed plaques were assessed using CLSM combined with vital fluorescence technique and SEM. Results: In this study accumulated dental plaque revealed varied plaque microflora vitality and thickness according to participant's oral hygiene. The thickness of plaque smears ranged from 40.32 to 140.72 µm and 65.00 to 128.88 µm for live (vital) and dead accumulated microorganisms, respectively. Meanwhile, the thickness of plaque on the appliance ranged from 101 µm to 653 µm. CLSM revealed both dead and vital bacteria on the surface of the dental plaque. In addition, SEM revealed layers of various bacterial aggregations in all dental plaques. Conclusion: This study offers a potent non-invasive tool to evaluate and assess the dental plaque biofilm, which is a very important factor in the development of dental caries.https://jdt.tums.ac.ir/index.php/jdt/article/view/194MicroscopyConfocalDental PlaqueElectronFluorescent Antibody Technique |
| spellingShingle | KA. Al-Salihi NABA. Tarmidzi Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy Frontiers in Dentistry Microscopy Confocal Dental Plaque Electron Fluorescent Antibody Technique |
| title | Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy |
| title_full | Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy |
| title_fullStr | Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy |
| title_full_unstemmed | Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy |
| title_short | Preliminary Study of In Vivo Formed Dental Plaque Using Confocal Microscopy and Scanning Electron Microscopy |
| title_sort | preliminary study of in vivo formed dental plaque using confocal microscopy and scanning electron microscopy |
| topic | Microscopy Confocal Dental Plaque Electron Fluorescent Antibody Technique |
| url | https://jdt.tums.ac.ir/index.php/jdt/article/view/194 |
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