Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks
Here the authors fuse hRad51 and variants thereof to Cas9 nickase to facilitate homology-directed repair without generating double strand breaks, minimizing indel formation and off-target editing. This tool represents progress towards the goal of performing HDR without an excess of undesired side pr...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-05-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-09983-4 |