The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability
Directed formation of the structure of the culture of living cells is the most important task of tissue engineering. New materials for 3D scaffolds of living tissue are critical for the mass adoption of regenerative medicine protocols. In this manuscript, we demonstrate the results of the molecular...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/5/1220 |
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| author | Veronika Anohova Lyudmila Asyakina Olga Babich Olga Dikaya Aleksandr Goikhman Ksenia Maksimova Margarita Grechkina Maxim Korobenkov Diana Burkova Aleksandr Barannikov Anton Narikovich Evgeny Chupakhin Anatoly Snigirev Sergey Antipov |
| author_facet | Veronika Anohova Lyudmila Asyakina Olga Babich Olga Dikaya Aleksandr Goikhman Ksenia Maksimova Margarita Grechkina Maxim Korobenkov Diana Burkova Aleksandr Barannikov Anton Narikovich Evgeny Chupakhin Anatoly Snigirev Sergey Antipov |
| author_sort | Veronika Anohova |
| collection | DOAJ |
| description | Directed formation of the structure of the culture of living cells is the most important task of tissue engineering. New materials for 3D scaffolds of living tissue are critical for the mass adoption of regenerative medicine protocols. In this manuscript, we demonstrate the results of the molecular structure study of collagen from <i>Dosidicus gigas</i> and reveal the possibility of obtaining a thin membrane material. The collagen membrane is characterized by high flexibility and plasticity as well as mechanical strength. The technology of obtaining collagen scaffolds, as well as the results of studies of its mechanical properties, surface morphology, protein composition, and the process of cell proliferation on its surface, are shown in the given manuscript. The investigation of living tissue culture grown on the surface of a collagen scaffold by X-ray tomography on a synchrotron source made it possible to remodel the structure of the extracellular matrix. It was found that the scaffolds obtained from squid collagen are characterized by a high degree of fibril ordering and high surface roughness and provide efficient directed growth of the cell culture. The resulting material provides the formation of the extracellular matrix and is characterized by a short time to living tissue sorption. |
| first_indexed | 2024-03-11T07:12:58Z |
| format | Article |
| id | doaj.art-be49bc08821d463cab82c684b3fba771 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T07:12:58Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-be49bc08821d463cab82c684b3fba7712023-11-17T08:27:51ZengMDPI AGPolymers2073-43602023-02-01155122010.3390/polym15051220The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell ViabilityVeronika Anohova0Lyudmila Asyakina1Olga Babich2Olga Dikaya3Aleksandr Goikhman4Ksenia Maksimova5Margarita Grechkina6Maxim Korobenkov7Diana Burkova8Aleksandr Barannikov9Anton Narikovich10Evgeny Chupakhin11Anatoly Snigirev12Sergey Antipov13Immanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaVoronezh State University, 1, University Square, Voronezh 394063, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaVoronezh State University, 1, University Square, Voronezh 394063, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaImmanuel Kant Baltic Federal University, Nevskogo 14, Kaliningrad 236006, RussiaDirected formation of the structure of the culture of living cells is the most important task of tissue engineering. New materials for 3D scaffolds of living tissue are critical for the mass adoption of regenerative medicine protocols. In this manuscript, we demonstrate the results of the molecular structure study of collagen from <i>Dosidicus gigas</i> and reveal the possibility of obtaining a thin membrane material. The collagen membrane is characterized by high flexibility and plasticity as well as mechanical strength. The technology of obtaining collagen scaffolds, as well as the results of studies of its mechanical properties, surface morphology, protein composition, and the process of cell proliferation on its surface, are shown in the given manuscript. The investigation of living tissue culture grown on the surface of a collagen scaffold by X-ray tomography on a synchrotron source made it possible to remodel the structure of the extracellular matrix. It was found that the scaffolds obtained from squid collagen are characterized by a high degree of fibril ordering and high surface roughness and provide efficient directed growth of the cell culture. The resulting material provides the formation of the extracellular matrix and is characterized by a short time to living tissue sorption.https://www.mdpi.com/2073-4360/15/5/1220collagen scaffoldsmechanical propertiesphysicochemical propertiesmorphologycompositioncell viability |
| spellingShingle | Veronika Anohova Lyudmila Asyakina Olga Babich Olga Dikaya Aleksandr Goikhman Ksenia Maksimova Margarita Grechkina Maxim Korobenkov Diana Burkova Aleksandr Barannikov Anton Narikovich Evgeny Chupakhin Anatoly Snigirev Sergey Antipov The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability Polymers collagen scaffolds mechanical properties physicochemical properties morphology composition cell viability |
| title | The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability |
| title_full | The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability |
| title_fullStr | The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability |
| title_full_unstemmed | The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability |
| title_short | The <i>Dosidicus gigas</i> Collagen for Scaffold Preparation and Cell Cultivation: Mechanical and Physicochemical Properties, Morphology, Composition and Cell Viability |
| title_sort | i dosidicus gigas i collagen for scaffold preparation and cell cultivation mechanical and physicochemical properties morphology composition and cell viability |
| topic | collagen scaffolds mechanical properties physicochemical properties morphology composition cell viability |
| url | https://www.mdpi.com/2073-4360/15/5/1220 |
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