Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model
Objectives: The objective of this study was to investigate the effect of botulinum toxin type A (BTX-A)–induced quadriceps muscle atrophy on the cartilage and subchondral bone in an otherwise intact rat joint model. Methods: The rat right quadriceps muscle atrophy was established by intramuscular in...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Journal of Orthopaedic Translation |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X19302153 |
| _version_ | 1818480127492751360 |
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| author | Jichun Xu Guorong She Tao Gui Huige Hou Jieruo Li Yuanfeng Chen Zhengang Zha |
| author_facet | Jichun Xu Guorong She Tao Gui Huige Hou Jieruo Li Yuanfeng Chen Zhengang Zha |
| author_sort | Jichun Xu |
| collection | DOAJ |
| description | Objectives: The objective of this study was to investigate the effect of botulinum toxin type A (BTX-A)–induced quadriceps muscle atrophy on the cartilage and subchondral bone in an otherwise intact rat joint model. Methods: The rat right quadriceps muscle atrophy was established by intramuscular injection of BTX-A. Twenty-four rats were divided randomly into 3 groups: The BTX-A–treated 4-week group; the BTX-A–treated 8-week group; and the control group injected with phosphate buffer saline were observed for 8 weeks. Muscle atrophy level was measured by weighing and histology examinations. Serum interleukin-1β level was tested by ELISA (enzyme linked immunosorbent assay); the subchondral bone was analysed by micro–computed tomography and the cartilage was measured by histology examinations (gross view, haematoxylin and eosin staining and Safranin-O/fast green staining) and immunohistochemistry test {collagen X [ColX]}. Results: BTX-A intramuscular injection led to muscle atrophy. Characteristics of muscle atrophy appeared in two BTX-A–injected groups but not in the control group. Quadriceps atrophy did not affect interleukin-1β level in serum, but resulted in subchondral bone abnormal changes with reduced bone volume/total tissue volume and increased Structure Model Index. Furthermore, the more the severe cartilage damage, the higher the histologic damage scores, followed by the higher the percentage of collagen X–positive chondrocytes caused by muscle atrophy. Conclusions: Quadriceps muscle atrophy triggered the subchondral bone abnormal change and cartilage degeneration, which would be a risk factor for development of osteoarthritis. The translational potential of this article: Our results indicate that anti-quadriceps muscle atrophy can be a candidate therapeutic target in the prevention of knee osteoarthritis. |
| first_indexed | 2024-12-10T11:19:06Z |
| format | Article |
| id | doaj.art-be4a46b4ce6947cdabd70a43fac0611f |
| institution | Directory Open Access Journal |
| issn | 2214-031X |
| language | English |
| last_indexed | 2024-12-10T11:19:06Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj.art-be4a46b4ce6947cdabd70a43fac0611f2022-12-22T01:51:03ZengElsevierJournal of Orthopaedic Translation2214-031X2020-05-01226772Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat modelJichun Xu0Guorong She1Tao Gui2Huige Hou3Jieruo Li4Yuanfeng Chen5Zhengang Zha6Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaInstitute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaCorresponding author. Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR China.; Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaCorresponding author. Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR China.; Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, PR ChinaObjectives: The objective of this study was to investigate the effect of botulinum toxin type A (BTX-A)–induced quadriceps muscle atrophy on the cartilage and subchondral bone in an otherwise intact rat joint model. Methods: The rat right quadriceps muscle atrophy was established by intramuscular injection of BTX-A. Twenty-four rats were divided randomly into 3 groups: The BTX-A–treated 4-week group; the BTX-A–treated 8-week group; and the control group injected with phosphate buffer saline were observed for 8 weeks. Muscle atrophy level was measured by weighing and histology examinations. Serum interleukin-1β level was tested by ELISA (enzyme linked immunosorbent assay); the subchondral bone was analysed by micro–computed tomography and the cartilage was measured by histology examinations (gross view, haematoxylin and eosin staining and Safranin-O/fast green staining) and immunohistochemistry test {collagen X [ColX]}. Results: BTX-A intramuscular injection led to muscle atrophy. Characteristics of muscle atrophy appeared in two BTX-A–injected groups but not in the control group. Quadriceps atrophy did not affect interleukin-1β level in serum, but resulted in subchondral bone abnormal changes with reduced bone volume/total tissue volume and increased Structure Model Index. Furthermore, the more the severe cartilage damage, the higher the histologic damage scores, followed by the higher the percentage of collagen X–positive chondrocytes caused by muscle atrophy. Conclusions: Quadriceps muscle atrophy triggered the subchondral bone abnormal change and cartilage degeneration, which would be a risk factor for development of osteoarthritis. The translational potential of this article: Our results indicate that anti-quadriceps muscle atrophy can be a candidate therapeutic target in the prevention of knee osteoarthritis.http://www.sciencedirect.com/science/article/pii/S2214031X19302153CartilageMuscle atrophyOsteoarthritisSubchondral bone |
| spellingShingle | Jichun Xu Guorong She Tao Gui Huige Hou Jieruo Li Yuanfeng Chen Zhengang Zha Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model Journal of Orthopaedic Translation Cartilage Muscle atrophy Osteoarthritis Subchondral bone |
| title | Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model |
| title_full | Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model |
| title_fullStr | Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model |
| title_full_unstemmed | Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model |
| title_short | Knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model |
| title_sort | knee muscle atrophy is a risk factor for development of knee osteoarthritis in a rat model |
| topic | Cartilage Muscle atrophy Osteoarthritis Subchondral bone |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X19302153 |
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