A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils
From the stand point of the possible use of microorganisms in water purifi cation biotechnologies, studying microorganisms capable of removing dangerous pollutants such as heavy metals from the aquatic environment is of great importance. Microbial screening was conducted on isolates from microbiocen...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Saratov State University
2022-12-01
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| Series: | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
| Subjects: | |
| Online Access: | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2022/12/himiya_2022_4-409-418.pdf |
| _version_ | 1797976031627313152 |
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| author | Pleshakova, Ekaterina Vladimirovna Kasatkina, Milena Александровна Ngun, Clement T. Reshetnikov, Michae V. |
| author_facet | Pleshakova, Ekaterina Vladimirovna Kasatkina, Milena Александровна Ngun, Clement T. Reshetnikov, Michae V. |
| author_sort | Pleshakova, Ekaterina Vladimirovna |
| collection | DOAJ |
| description | From the stand point of the possible use of microorganisms in water purifi cation biotechnologies, studying microorganisms capable of removing dangerous pollutants such as heavy metals from the aquatic environment is of great importance. Microbial screening was conducted on isolates from microbiocenoses of highly magnetic soils of the city Mednogorsk (Orenburg region, Russia), to assess their resistance to manganese (II) ions. The objects of this research were nine strains of manganese-oxidizing microorganisms and two strains of iron-oxidizing microorganisms: Bacillus megaterium 69.3 and B. megaterium 69.5. It was observed that, most of the studied microorganisms were characterized by increased resistance to manganese (II) ions when grown on an agar medium. Maximum resistance to Mn (II) was observed in microbial strains: 55.2 and B. megaterium 69.5. The maximum tolerant concentration (MTC) of Mn (II) for these microorganisms was 300 and 350 mmol/L; the minimum inhibitory concentration (MIC) was 350 and 450 mmol/L, respectively. When studying the growth of the two microbial strains: 55.2 and B. megaterium 69.5 in liquid media for 5 days. It was discovered that, when cultured in a selective medium, microbial resistance of the strains to Mn (II) was higher compared to when cultured in a nutrient LB medium. The results showed high resistance of microorganisms to a range of Mn (II) concentrations: from 0,5 to 250 mmol/L in a liquid medium. Maximum growth of the studied microbial strains was observed at Mn (II) concentration of 10 mmol/L. These microbial strains with high resistance to Mn (II) open up the prospect of their practical use for biotechnological purposes involving the purifi cation of drinking and wastewater from high manganese content. |
| first_indexed | 2024-04-11T04:44:51Z |
| format | Article |
| id | doaj.art-be4e400a34af4e99bac761ad678cad80 |
| institution | Directory Open Access Journal |
| issn | 1816-9775 2541-8971 |
| language | English |
| last_indexed | 2024-04-11T04:44:51Z |
| publishDate | 2022-12-01 |
| publisher | Saratov State University |
| record_format | Article |
| series | Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология |
| spelling | doaj.art-be4e400a34af4e99bac761ad678cad802022-12-27T13:54:16ZengSaratov State UniversityИзвестия Саратовского университета. Новая серия: Серия Химия. Биология. Экология1816-97752541-89712022-12-0122440941810.18500/1816-9775-2022-22-4-409-418A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soilsPleshakova, Ekaterina Vladimirovna0Kasatkina, Milena Александровна1Ngun, Clement T.2Reshetnikov, Michae V.3Saratov State University, 83, Astrakhanskaya str., Saratov, 410012, RussiaSaratov State University, 83, Astrakhanskaya str., Saratov, 410012, RussiaSaratov State University, 83, Astrakhanskaya str., Saratov, 410012, RussiaSaratov State University, 83, Astrakhanskaya str., Saratov, 410012, RussiaFrom the stand point of the possible use of microorganisms in water purifi cation biotechnologies, studying microorganisms capable of removing dangerous pollutants such as heavy metals from the aquatic environment is of great importance. Microbial screening was conducted on isolates from microbiocenoses of highly magnetic soils of the city Mednogorsk (Orenburg region, Russia), to assess their resistance to manganese (II) ions. The objects of this research were nine strains of manganese-oxidizing microorganisms and two strains of iron-oxidizing microorganisms: Bacillus megaterium 69.3 and B. megaterium 69.5. It was observed that, most of the studied microorganisms were characterized by increased resistance to manganese (II) ions when grown on an agar medium. Maximum resistance to Mn (II) was observed in microbial strains: 55.2 and B. megaterium 69.5. The maximum tolerant concentration (MTC) of Mn (II) for these microorganisms was 300 and 350 mmol/L; the minimum inhibitory concentration (MIC) was 350 and 450 mmol/L, respectively. When studying the growth of the two microbial strains: 55.2 and B. megaterium 69.5 in liquid media for 5 days. It was discovered that, when cultured in a selective medium, microbial resistance of the strains to Mn (II) was higher compared to when cultured in a nutrient LB medium. The results showed high resistance of microorganisms to a range of Mn (II) concentrations: from 0,5 to 250 mmol/L in a liquid medium. Maximum growth of the studied microbial strains was observed at Mn (II) concentration of 10 mmol/L. These microbial strains with high resistance to Mn (II) open up the prospect of their practical use for biotechnological purposes involving the purifi cation of drinking and wastewater from high manganese content.https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2022/12/himiya_2022_4-409-418.pdfmanganese (ii) ionsmanganese-oxidizing microorganismsresistancemaximum tolerant concentrationminimum inhibitory concentration |
| spellingShingle | Pleshakova, Ekaterina Vladimirovna Kasatkina, Milena Александровна Ngun, Clement T. Reshetnikov, Michae V. A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils Известия Саратовского университета. Новая серия: Серия Химия. Биология. Экология manganese (ii) ions manganese-oxidizing microorganisms resistance maximum tolerant concentration minimum inhibitory concentration |
| title | A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils |
| title_full | A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils |
| title_fullStr | A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils |
| title_full_unstemmed | A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils |
| title_short | A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils |
| title_sort | study of manganese oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils |
| topic | manganese (ii) ions manganese-oxidizing microorganisms resistance maximum tolerant concentration minimum inhibitory concentration |
| url | https://ichbe.sgu.ru/sites/ichbe.sgu.ru/files/text-pdf/2022/12/himiya_2022_4-409-418.pdf |
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