Knockdown of PLAC1 inhibits BCa growth and migration through upregulation of FOXO3a
Placenta-specific protein 1 (PLAC1) is considered to play a pivotal role in cancer progression. Here, we investigated the role of PLAC1 in the growth and motility of bladder cancer (BCa) cells. Database analysis and Immunoblot assays were conducted to determine PLAC1 expression in BCa tissues and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MRE Press
2024-02-01
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| Series: | Journal of Men's Health |
| Subjects: | |
| Online Access: | https://oss.jomh.org/files/article/20240229-210/pdf/JOMH2023121301.pdf |
| Summary: | Placenta-specific protein 1 (PLAC1) is considered to play a pivotal role in
cancer progression. Here, we investigated the role of PLAC1 in the growth and
motility of bladder cancer (BCa) cells. Database analysis and Immunoblot assays
were conducted to determine PLAC1 expression in BCa tissues and its correlation
with patient prognosis. Furthermore, wound healing, transwell and tube formation
assays were performed to evaluate cell motility and angiogenic potential, and the
underlying mechanism via which PLAC1 knockdown inhibits BCa progression
in vitro was investigated. The data revealed that PLAC1 was obviously
overexpressed in BCa tissues and was associated with poor patient prognoses.
Additionally, silencing PLAC1 led to reduced viability, migratory capacity,
invasion potential and angiogenesis of BCa cells, including T24 and UMUC3 cells.
Further investigation showed that PLAC1 knockdown modulated the
phosphoinositide 3-kinase/protein kinase B/forkhead box O3a
(PI3K/Akt/FOXO3a) axis by enhancing the phosphorylation of FOXO3a while
suppressing the phosphorylation of PI3K as well as Akt. Moreover, we demonstrated
that the inhibition of BCa progression by PLAC1 knockdown was primarily mediated
through the targeting of FOXO3a. In summary, these findings confirmed the
potential of PLAC1 as a promising target for suppressing BCa growth by elevating
FOXO3a levels and modulating the PI3K/Akt/FOXO3a signaling axis. |
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| ISSN: | 1875-6867 1875-6859 |