Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay
Antimicrobial peptides (AMPs) are used to treat many infectious diseases. Nowadays, AMPs enjoy special attention due to their novel mode of action and kill various pathogens such as fungi, bacteria and viruses. Among insects, the ant genus can provide a potential antimicrobial peptide against variou...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853522300624X |
| _version_ | 1797690950132170752 |
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| author | Hubballi Aravinda Kadanakuppe Thammayya Shivakumara Krishanappa Chandrashekara Abalavadi Thammaiah Rani Ryan Casini Shaban R.M. Sayed Hosam O. Elansary Ahmed M. El-Sabrout |
| author_facet | Hubballi Aravinda Kadanakuppe Thammayya Shivakumara Krishanappa Chandrashekara Abalavadi Thammaiah Rani Ryan Casini Shaban R.M. Sayed Hosam O. Elansary Ahmed M. El-Sabrout |
| author_sort | Hubballi Aravinda |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) are used to treat many infectious diseases. Nowadays, AMPs enjoy special attention due to their novel mode of action and kill various pathogens such as fungi, bacteria and viruses. Among insects, the ant genus can provide a potential antimicrobial peptide against various microorganisms. In the present study, AMPs compounds were isolated and purified from the ant species Trichomyrmex scabriceps using reversed-phase high performance liquid chromatography (RP-HPLC). The extract from abdomen showed the highest protein content (4.60 µg/µl) compared to head and thorax. The HPLC profile of the abdomen showed 14 different peaks, of which the peaks eluting at the 6th, 8th, 17th and 23rd minutes had a higher intensity. The number of peaks was comparatively lower in the extracts from the head and thorax. The antimicrobial activity of the head, thorax and abdomen extracts of ants was evaluated against Gram-positive Staphylococcus aureus (MTCC 3160) and Gram-negative Escherichia coli (MTCC 2692) bacterial strains. Among the extracts tested, the abdomen alone exhibited a distinct zone of inhibition against E. coli (176.98 ± 3.36 mm2). However, the extracts of the head and thorax showed no zone of inhibition. In addition, none of the extracts showed a zone of inhibition against S. aureus. To our knowledge, this is the first report observed AMPs in T. scabriceps ant and their antimicrobial property against these bacterial strains. |
| first_indexed | 2024-03-12T02:06:29Z |
| format | Article |
| id | doaj.art-be5310b257d74b34a5afc4241d330695 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T02:06:29Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-be5310b257d74b34a5afc4241d3306952023-09-07T04:43:36ZengElsevierArabian Journal of Chemistry1878-53522023-10-011610105162Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assayHubballi Aravinda0Kadanakuppe Thammayya Shivakumara1Krishanappa Chandrashekara2Abalavadi Thammaiah Rani3Ryan Casini4Shaban R.M. Sayed5Hosam O. Elansary6Ahmed M. El-Sabrout7Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, Bengaluru 560065, IndiaICAR-National Bureau of Agricultural Insect Resources, Bengaluru, Karnataka 560024, India; Corresponding authors.Department of Agricultural Entomology, University of Agricultural Sciences, GKVK, Bengaluru 560065, IndiaCentral Horticultural Experiment Station (ICAR-IIHR), Chettalli, Kodagu, Karnataka 571248, IndiaSchool of Public Health, University of California, Berkeley, 2121 Berkeley Way, Berkeley, CA 94704, USADepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia; Corresponding authors.Department of Applied Entomology and Zoology, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria 21545, EgyptAntimicrobial peptides (AMPs) are used to treat many infectious diseases. Nowadays, AMPs enjoy special attention due to their novel mode of action and kill various pathogens such as fungi, bacteria and viruses. Among insects, the ant genus can provide a potential antimicrobial peptide against various microorganisms. In the present study, AMPs compounds were isolated and purified from the ant species Trichomyrmex scabriceps using reversed-phase high performance liquid chromatography (RP-HPLC). The extract from abdomen showed the highest protein content (4.60 µg/µl) compared to head and thorax. The HPLC profile of the abdomen showed 14 different peaks, of which the peaks eluting at the 6th, 8th, 17th and 23rd minutes had a higher intensity. The number of peaks was comparatively lower in the extracts from the head and thorax. The antimicrobial activity of the head, thorax and abdomen extracts of ants was evaluated against Gram-positive Staphylococcus aureus (MTCC 3160) and Gram-negative Escherichia coli (MTCC 2692) bacterial strains. Among the extracts tested, the abdomen alone exhibited a distinct zone of inhibition against E. coli (176.98 ± 3.36 mm2). However, the extracts of the head and thorax showed no zone of inhibition. In addition, none of the extracts showed a zone of inhibition against S. aureus. To our knowledge, this is the first report observed AMPs in T. scabriceps ant and their antimicrobial property against these bacterial strains.http://www.sciencedirect.com/science/article/pii/S187853522300624XTrichomyrmex scabricepsFormicidaeAntimicrobial peptidesRP-HPLCEscherichia coliStaphylococcus aureus |
| spellingShingle | Hubballi Aravinda Kadanakuppe Thammayya Shivakumara Krishanappa Chandrashekara Abalavadi Thammaiah Rani Ryan Casini Shaban R.M. Sayed Hosam O. Elansary Ahmed M. El-Sabrout Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay Arabian Journal of Chemistry Trichomyrmex scabriceps Formicidae Antimicrobial peptides RP-HPLC Escherichia coli Staphylococcus aureus |
| title | Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay |
| title_full | Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay |
| title_fullStr | Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay |
| title_full_unstemmed | Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay |
| title_short | Isolation of antimicrobial peptides from seed harvester ant, Trichomyrmex scabriceps (Mayr) (Hymenoptera: Formicidae) and their antimicrobial assay |
| title_sort | isolation of antimicrobial peptides from seed harvester ant trichomyrmex scabriceps mayr hymenoptera formicidae and their antimicrobial assay |
| topic | Trichomyrmex scabriceps Formicidae Antimicrobial peptides RP-HPLC Escherichia coli Staphylococcus aureus |
| url | http://www.sciencedirect.com/science/article/pii/S187853522300624X |
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