<sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice
Atherosclerosis is the most common disease of the vascular system and the metabolic disorder is one of its important molecular mechanisms. SAP protein is found to be highly expressed in atherosclerotic blood vessels. Our previous study found that SAP deficiency can significantly inhibit the developm...
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2022-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/12/1278 |
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| author | Qian Li Wanting Chen Wenbin Huang Ranran Hou Xinping Huang Man Xu Limei Que Lijing Wang Yongxia Yang |
| author_facet | Qian Li Wanting Chen Wenbin Huang Ranran Hou Xinping Huang Man Xu Limei Que Lijing Wang Yongxia Yang |
| author_sort | Qian Li |
| collection | DOAJ |
| description | Atherosclerosis is the most common disease of the vascular system and the metabolic disorder is one of its important molecular mechanisms. SAP protein is found to be highly expressed in atherosclerotic blood vessels. Our previous study found that SAP deficiency can significantly inhibit the development of atherosclerosis. However, the regulatory effect of SAP deficiency on AS metabolism is unknown. Based on <sup>1</sup>H-NMR metabonomics, this study investigated the serum metabolic changes in ApoE<sup>−/−</sup>;SAP<sup>−/−</sup> mice compared with ApoE<sup>−/−</sup> mice during the whole progression of atherosclerosis. The results showed that acetate, pyruvate, choline and VLDL + LDL were statistically regulated to the normal levels as in C57 mice by SAP deficiency in ApoE<sup>−/−</sup>;SAP<sup>−/−</sup> mice at 8 w (without obvious plaques). With the appearance and aggravation of atherosclerotic plaques (8 + 4 w and 8 + 8 w), the four metabolites of acetate, pyruvate, choline and VLDL + LDL were continuously regulated, which were denoted as the metabolic regulatory markers of SAP deficiency. We also found that the changes in these four metabolites had nothing to do with high-fat diet. Therefore, it was revealed that SAP deficiency regulated the metabolic disorders in ApoE<sup>−/−</sup> prior to the appearance of obvious atherosclerotic plaques, which is one of the important mechanisms leading to the inhibition of atherosclerosis, providing a new basis for the application of SAP in atherosclerosis. |
| first_indexed | 2024-03-09T16:06:30Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T16:06:30Z |
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| spelling | doaj.art-be53a3770454472d95fcc891e4012c292023-11-24T16:38:45ZengMDPI AGMetabolites2218-19892022-12-011212127810.3390/metabo12121278<sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> MiceQian Li0Wanting Chen1Wenbin Huang2Ranran Hou3Xinping Huang4Man Xu5Limei Que6Lijing Wang7Yongxia Yang8School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaDepartment of Breast Care Surgery, The First Affiliated Hospital, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaFoshan Fosun Chancheng Hospital, Foshan 528031, ChinaSchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Medical Information and Engineering, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaAtherosclerosis is the most common disease of the vascular system and the metabolic disorder is one of its important molecular mechanisms. SAP protein is found to be highly expressed in atherosclerotic blood vessels. Our previous study found that SAP deficiency can significantly inhibit the development of atherosclerosis. However, the regulatory effect of SAP deficiency on AS metabolism is unknown. Based on <sup>1</sup>H-NMR metabonomics, this study investigated the serum metabolic changes in ApoE<sup>−/−</sup>;SAP<sup>−/−</sup> mice compared with ApoE<sup>−/−</sup> mice during the whole progression of atherosclerosis. The results showed that acetate, pyruvate, choline and VLDL + LDL were statistically regulated to the normal levels as in C57 mice by SAP deficiency in ApoE<sup>−/−</sup>;SAP<sup>−/−</sup> mice at 8 w (without obvious plaques). With the appearance and aggravation of atherosclerotic plaques (8 + 4 w and 8 + 8 w), the four metabolites of acetate, pyruvate, choline and VLDL + LDL were continuously regulated, which were denoted as the metabolic regulatory markers of SAP deficiency. We also found that the changes in these four metabolites had nothing to do with high-fat diet. Therefore, it was revealed that SAP deficiency regulated the metabolic disorders in ApoE<sup>−/−</sup> prior to the appearance of obvious atherosclerotic plaques, which is one of the important mechanisms leading to the inhibition of atherosclerosis, providing a new basis for the application of SAP in atherosclerosis.https://www.mdpi.com/2218-1989/12/12/1278atherosclerosisSAP<sup>1</sup>H-NMRmetabonomics |
| spellingShingle | Qian Li Wanting Chen Wenbin Huang Ranran Hou Xinping Huang Man Xu Limei Que Lijing Wang Yongxia Yang <sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice Metabolites atherosclerosis SAP <sup>1</sup>H-NMR metabonomics |
| title | <sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice |
| title_full | <sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice |
| title_fullStr | <sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice |
| title_full_unstemmed | <sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice |
| title_short | <sup>1</sup>H-NMR-Based Metabonomics Study to Reveal the Progressive Metabolism Regulation of SAP Deficiency on ApoE<sup>−/−</sup> Mice |
| title_sort | sup 1 sup h nmr based metabonomics study to reveal the progressive metabolism regulation of sap deficiency on apoe sup sup mice |
| topic | atherosclerosis SAP <sup>1</sup>H-NMR metabonomics |
| url | https://www.mdpi.com/2218-1989/12/12/1278 |
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