Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646
The study addresses the growth of the wild-type strain <i>Cupriavidus necator</i> B-10646 and the synthesis of sulfur-containing polyhydroxyalkanoates (PHA) by this strain on media containing fructose and three different precursors (3-mercaptopropionic acid, 3′,3′-dithiodipropionic acid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/4/1005 |
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| author | Natalia O. Zhila Kristina Yu. Sapozhnikova Arina V. Berezovskaya Evgeniy G. Kiselev Ekaterina I. Shishatskaya Aleksander D. Vasiliev Sabu Thomas Tatiana G. Volova |
| author_facet | Natalia O. Zhila Kristina Yu. Sapozhnikova Arina V. Berezovskaya Evgeniy G. Kiselev Ekaterina I. Shishatskaya Aleksander D. Vasiliev Sabu Thomas Tatiana G. Volova |
| author_sort | Natalia O. Zhila |
| collection | DOAJ |
| description | The study addresses the growth of the wild-type strain <i>Cupriavidus necator</i> B-10646 and the synthesis of sulfur-containing polyhydroxyalkanoates (PHA) by this strain on media containing fructose and three different precursors (3-mercaptopropionic acid, 3′,3′-dithiodipropionic acid and 3′,3′-thiodipropionic acid). By varying the concentration and number of doses of the precursors added into the bacterial culture, it was possible to find conditions that ensure the formation of 3-mercaptopropionate (3MP) monomers from the precursors and their incorporation into the C-chain of poly(3-hydroxybutyrate). A series of P(3HB-<i>co</i>-3MP) copolymer samples with different content of 3MP monomers (from 2.04 to 39.0 mol.%) were synthesized and the physicochemical properties were studied. The effect of 3MP monomers is manifested in a certain decrease in the molecular weight of the samples and an increase in polydispersity. Temperature changes are manifested in the appearance of two peaks in the melting region with different intervals regardless of the 3MP content. The studied P(3HB-<i>co</i>-3MP) samples, regardless of the content of 3MP monomers, are characterized by equalization of the ratio of the amorphous and crystalline phases and have a close degree of crystallinity with a minimum of 42%, = and a maximum of 54%. |
| first_indexed | 2024-03-11T08:14:08Z |
| format | Article |
| id | doaj.art-be54d090e9304f469b30914464b5978c |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T08:14:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-be54d090e9304f469b30914464b5978c2023-11-16T22:52:52ZengMDPI AGPolymers2073-43602023-02-01154100510.3390/polym15041005Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646Natalia O. Zhila0Kristina Yu. Sapozhnikova1Arina V. Berezovskaya2Evgeniy G. Kiselev3Ekaterina I. Shishatskaya4Aleksander D. Vasiliev5Sabu Thomas6Tatiana G. Volova7Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaL.V. Kirensky Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/38 Akademgorodok, 660036 Krasnoyarsk, RussiaBasic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., 660041 Krasnoyarsk, RussiaInstitute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, 660036 Krasnoyarsk, RussiaThe study addresses the growth of the wild-type strain <i>Cupriavidus necator</i> B-10646 and the synthesis of sulfur-containing polyhydroxyalkanoates (PHA) by this strain on media containing fructose and three different precursors (3-mercaptopropionic acid, 3′,3′-dithiodipropionic acid and 3′,3′-thiodipropionic acid). By varying the concentration and number of doses of the precursors added into the bacterial culture, it was possible to find conditions that ensure the formation of 3-mercaptopropionate (3MP) monomers from the precursors and their incorporation into the C-chain of poly(3-hydroxybutyrate). A series of P(3HB-<i>co</i>-3MP) copolymer samples with different content of 3MP monomers (from 2.04 to 39.0 mol.%) were synthesized and the physicochemical properties were studied. The effect of 3MP monomers is manifested in a certain decrease in the molecular weight of the samples and an increase in polydispersity. Temperature changes are manifested in the appearance of two peaks in the melting region with different intervals regardless of the 3MP content. The studied P(3HB-<i>co</i>-3MP) samples, regardless of the content of 3MP monomers, are characterized by equalization of the ratio of the amorphous and crystalline phases and have a close degree of crystallinity with a minimum of 42%, = and a maximum of 54%.https://www.mdpi.com/2073-4360/15/4/1005biosynthesisdifferent precursorssulfur-containing PHAschemical composition3MP monomers contentsproperties |
| spellingShingle | Natalia O. Zhila Kristina Yu. Sapozhnikova Arina V. Berezovskaya Evgeniy G. Kiselev Ekaterina I. Shishatskaya Aleksander D. Vasiliev Sabu Thomas Tatiana G. Volova Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646 Polymers biosynthesis different precursors sulfur-containing PHAs chemical composition 3MP monomers contents properties |
| title | Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646 |
| title_full | Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646 |
| title_fullStr | Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646 |
| title_full_unstemmed | Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646 |
| title_short | Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain <i>Cupriavidus necator</i> B-10646 |
| title_sort | biosynthesis and properties of sulfur containing polyhydroxyalkanoates phas produced by wild type strain i cupriavidus necator i b 10646 |
| topic | biosynthesis different precursors sulfur-containing PHAs chemical composition 3MP monomers contents properties |
| url | https://www.mdpi.com/2073-4360/15/4/1005 |
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