Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i>
The authors of this paper conducted a comparative metabolomic analysis of <i>Ophiocordyceps sinensis</i> (OS), providing the metabolic profiles of the stroma (OSBSz) and sclerotia (OSBSh) of OS by widely targeted metabolomics and untargeted metabolomics. The results showed that 778 and 1...
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| author | Jinna Zhou Donghai Hou Weiqiu Zou Jinhu Wang Run Luo Mu Wang Hong Yu |
| author_facet | Jinna Zhou Donghai Hou Weiqiu Zou Jinhu Wang Run Luo Mu Wang Hong Yu |
| author_sort | Jinna Zhou |
| collection | DOAJ |
| description | The authors of this paper conducted a comparative metabolomic analysis of <i>Ophiocordyceps sinensis</i> (OS), providing the metabolic profiles of the stroma (OSBSz) and sclerotia (OSBSh) of OS by widely targeted metabolomics and untargeted metabolomics. The results showed that 778 and 1449 metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. The metabolites in OSBSz and OSBSh are significantly differentiated; 71 and 96 differentially expressed metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. This suggests that these 71 metabolites (riboflavine, tripdiolide, bromocriptine, lumichrome, tetrahymanol, citrostadienol, etc.) and 96 metabolites (sancycline, vignatic acid B, pirbuterol, rubrophen, epalrestat, etc.) are potential biomarkers. 4-Hydroxybenzaldehyde, arginine, and lumichrome were common differentially expressed metabolites. Using the widely targeted metabolomics approach, the key pathways identified that are involved in creating the differentiation between OSBSz and OSBSh may be nicotinate and nicotinamide metabolism, thiamine metabolism, riboflavin metabolism, glycine, serine, and threonine metabolism, and arginine biosynthesis. The differentially expressed metabolites identified using the untargeted metabolomics approach were mainly involved in arginine biosynthesis, terpenoid backbone biosynthesis, porphyrin and chlorophyll metabolism, and cysteine and methionine metabolism. The purpose of this research was to provide support for the assessment of the differences between the stroma and sclerotia, to furnish a material basis for the evaluation of the physical effects of OS, and to provide a reference for the selection of detection methods for the metabolomics of OS. |
| first_indexed | 2024-03-10T01:02:50Z |
| format | Article |
| id | doaj.art-be582ca6efcc4b7289ff2ce94cbafce5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:02:50Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-be582ca6efcc4b7289ff2ce94cbafce52023-11-23T14:32:08ZengMDPI AGMolecules1420-30492022-06-012711364510.3390/molecules27113645Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i>Jinna Zhou0Donghai Hou1Weiqiu Zou2Jinhu Wang3Run Luo4Mu Wang5Hong Yu6College of Science, Tibet University, Lhasa 850001, ChinaSchool of Life Sciences, Yunnan University, Kunming 650106, ChinaSchool of Life Sciences, Yunnan University, Kunming 650106, ChinaCollege of Science, Tibet University, Lhasa 850001, ChinaSchool of Life Sciences, Yunnan University, Kunming 650106, ChinaPlant Science College, Tibet Agriculture & Animal Husbandry University, Nyingchi 851418, ChinaYunnan Herbal Laboratory, College of Ecology and Environmental Sciences, Yunnan University, Kunming 650106, ChinaThe authors of this paper conducted a comparative metabolomic analysis of <i>Ophiocordyceps sinensis</i> (OS), providing the metabolic profiles of the stroma (OSBSz) and sclerotia (OSBSh) of OS by widely targeted metabolomics and untargeted metabolomics. The results showed that 778 and 1449 metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. The metabolites in OSBSz and OSBSh are significantly differentiated; 71 and 96 differentially expressed metabolites were identified by the widely targeted metabolomics and untargeted metabolomics approaches, respectively. This suggests that these 71 metabolites (riboflavine, tripdiolide, bromocriptine, lumichrome, tetrahymanol, citrostadienol, etc.) and 96 metabolites (sancycline, vignatic acid B, pirbuterol, rubrophen, epalrestat, etc.) are potential biomarkers. 4-Hydroxybenzaldehyde, arginine, and lumichrome were common differentially expressed metabolites. Using the widely targeted metabolomics approach, the key pathways identified that are involved in creating the differentiation between OSBSz and OSBSh may be nicotinate and nicotinamide metabolism, thiamine metabolism, riboflavin metabolism, glycine, serine, and threonine metabolism, and arginine biosynthesis. The differentially expressed metabolites identified using the untargeted metabolomics approach were mainly involved in arginine biosynthesis, terpenoid backbone biosynthesis, porphyrin and chlorophyll metabolism, and cysteine and methionine metabolism. The purpose of this research was to provide support for the assessment of the differences between the stroma and sclerotia, to furnish a material basis for the evaluation of the physical effects of OS, and to provide a reference for the selection of detection methods for the metabolomics of OS.https://www.mdpi.com/1420-3049/27/11/3645metabolomics<i>Ophiocordyceps sinensis</i>widely targeted metabolomicsuntargeted metabolomicsFungi |
| spellingShingle | Jinna Zhou Donghai Hou Weiqiu Zou Jinhu Wang Run Luo Mu Wang Hong Yu Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i> Molecules metabolomics <i>Ophiocordyceps sinensis</i> widely targeted metabolomics untargeted metabolomics Fungi |
| title | Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i> |
| title_full | Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i> |
| title_fullStr | Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i> |
| title_full_unstemmed | Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i> |
| title_short | Comparison of Widely Targeted Metabolomics and Untargeted Metabolomics of Wild <i>Ophiocordyceps sinensis</i> |
| title_sort | comparison of widely targeted metabolomics and untargeted metabolomics of wild i ophiocordyceps sinensis i |
| topic | metabolomics <i>Ophiocordyceps sinensis</i> widely targeted metabolomics untargeted metabolomics Fungi |
| url | https://www.mdpi.com/1420-3049/27/11/3645 |
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