Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes
Obesity is characterized by excessive lipid accumulation, hypertrophy, and hyperplasia of adipose cells. Hulless barley (Hordeum vulgare L. var. nudum Hook. f.) is the principal crop grown in the Qinghai-Tibet plateau. Polyphenols, the major bioactive compound in hulless barley, possess antioxidant,...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.933068/full |
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| author | Xianfeng Deng Bi Chen Qin Luo Xingru Zao Haizhe Liu Yongqiang Li |
| author_facet | Xianfeng Deng Bi Chen Qin Luo Xingru Zao Haizhe Liu Yongqiang Li |
| author_sort | Xianfeng Deng |
| collection | DOAJ |
| description | Obesity is characterized by excessive lipid accumulation, hypertrophy, and hyperplasia of adipose cells. Hulless barley (Hordeum vulgare L. var. nudum Hook. f.) is the principal crop grown in the Qinghai-Tibet plateau. Polyphenols, the major bioactive compound in hulless barley, possess antioxidant, anti-inflammatory, and antibacterial properties. However, the anti-obesity effect of hulless barley polyphenol (HBP) extract has not been explored. Therefore, the current study assessed the impact of HBP extract on preventing obesity. For this purpose, we evaluated the inhibitory effect of HBP extract against obesity-related enzymes. Moreover, we investigated the effect of HBP extract on adipocyte differentiation and adipogenesis through 3T3-L1 adipocytes. Our results demonstrated that HBP extract could inhibit α-amylase, α-glucosidase (α-GLU), and lipase in a dose-dependent manner. In addition, HBP extract inhibited the differentiation of 3T3-L1 preadipocytes by arresting the cell cycle at the G0/G1 phase. Furthermore, the extract suppressed the expression of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), regulating fatty acid synthase (FAS), fatty acid-binding protein 4 (FABP4), and adipose triglyceride lipase (ATGL). It was also observed that HBP extract alleviated intracellular lipid accumulation by attenuating oxidative stress. These findings specify that HBP extract could inhibit obesity-related enzymes, adipocyte differentiation, and adipogenesis. Therefore, it is potentially beneficial in preventing obesity. |
| first_indexed | 2024-12-10T17:19:47Z |
| format | Article |
| id | doaj.art-be585a48cb6e4ce2ab639f3f103ed70f |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-10T17:19:47Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-be585a48cb6e4ce2ab639f3f103ed70f2022-12-22T01:40:00ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-08-01910.3389/fnut.2022.933068933068Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymesXianfeng Deng0Bi Chen1Qin Luo2Xingru Zao3Haizhe Liu4Yongqiang Li5College of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaSchool of Life and Health Science, Kaili University, Kaili, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaObesity is characterized by excessive lipid accumulation, hypertrophy, and hyperplasia of adipose cells. Hulless barley (Hordeum vulgare L. var. nudum Hook. f.) is the principal crop grown in the Qinghai-Tibet plateau. Polyphenols, the major bioactive compound in hulless barley, possess antioxidant, anti-inflammatory, and antibacterial properties. However, the anti-obesity effect of hulless barley polyphenol (HBP) extract has not been explored. Therefore, the current study assessed the impact of HBP extract on preventing obesity. For this purpose, we evaluated the inhibitory effect of HBP extract against obesity-related enzymes. Moreover, we investigated the effect of HBP extract on adipocyte differentiation and adipogenesis through 3T3-L1 adipocytes. Our results demonstrated that HBP extract could inhibit α-amylase, α-glucosidase (α-GLU), and lipase in a dose-dependent manner. In addition, HBP extract inhibited the differentiation of 3T3-L1 preadipocytes by arresting the cell cycle at the G0/G1 phase. Furthermore, the extract suppressed the expression of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), regulating fatty acid synthase (FAS), fatty acid-binding protein 4 (FABP4), and adipose triglyceride lipase (ATGL). It was also observed that HBP extract alleviated intracellular lipid accumulation by attenuating oxidative stress. These findings specify that HBP extract could inhibit obesity-related enzymes, adipocyte differentiation, and adipogenesis. Therefore, it is potentially beneficial in preventing obesity.https://www.frontiersin.org/articles/10.3389/fnut.2022.933068/fullhulless barleypolyphenolsenzymes3T3-L1 cellsadipogenesis |
| spellingShingle | Xianfeng Deng Bi Chen Qin Luo Xingru Zao Haizhe Liu Yongqiang Li Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes Frontiers in Nutrition hulless barley polyphenols enzymes 3T3-L1 cells adipogenesis |
| title | Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes |
| title_full | Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes |
| title_fullStr | Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes |
| title_full_unstemmed | Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes |
| title_short | Hulless barley polyphenol extract inhibits adipogenesis in 3T3-L1 cells and obesity related-enzymes |
| title_sort | hulless barley polyphenol extract inhibits adipogenesis in 3t3 l1 cells and obesity related enzymes |
| topic | hulless barley polyphenols enzymes 3T3-L1 cells adipogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.933068/full |
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