Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper
Cucumber mosaic virus (CMV), the most prevalent virus in chili pepper (Capsicum annuum L.), negatively affects chili pepper production in South Korea. In this study, foliar spraying with β-glucans obtained from the mycelial walls of the yeast-like fungus Aureobasidium pullulans inhibited CMV infecti...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2021-03-01
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| Series: | Research in Plant Disease |
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| Online Access: | http://www.online-rpd.org/upload/pdf/RPD-2021-27-1-17.pdf |
| _version_ | 1818354246014205952 |
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| author | Ju-Yeon Yoon V. S. R. Gangireddygari In-Sook Cho Bong-Nam Chung Byung-Dae Yoon Seung-Kook Choi |
| author_facet | Ju-Yeon Yoon V. S. R. Gangireddygari In-Sook Cho Bong-Nam Chung Byung-Dae Yoon Seung-Kook Choi |
| author_sort | Ju-Yeon Yoon |
| collection | DOAJ |
| description | Cucumber mosaic virus (CMV), the most prevalent virus in chili pepper (Capsicum annuum L.), negatively affects chili pepper production in South Korea. In this study, foliar spraying with β-glucans obtained from the mycelial walls of the yeast-like fungus Aureobasidium pullulans inhibited CMV infection of chili pepper if applied before virus inoculation. At three concentrations, β-glucans from A. pullulans significantly ameliorated CMV symptoms in treated chili pepper; the effect was greater in plants treated with 0.01% β-glucans than 0.005% or 0.001% β-glucans. Double antibody sandwich enzyme-linked immunosorbent assay showed that these β-glucans treatments resulted in 1.7- to 10-fold reductions in CMV accumulation in the treated chili pepper. The glucans did not act directly on the virus and did not interfere with virus disassembly or replication. Foliar spraying with 0.01% β-glucans from A. pullulans at 24 hr intervals for 3 days significantly increased plant height, the total number of fruit, and the fresh weight of chili pepper fruit. However, the stem diameter of chili pepper treated with β-glucans did not increase significantly. These results indicate that foliar spraying with β-glucans from A. pullulans acts an antiviral agent against CMV infection and stimulates chili pepper growth. |
| first_indexed | 2024-12-13T19:22:22Z |
| format | Article |
| id | doaj.art-be58ee19175d459f8b084abb0cd51599 |
| institution | Directory Open Access Journal |
| issn | 1598-2262 2233-9191 |
| language | English |
| last_indexed | 2024-12-13T19:22:22Z |
| publishDate | 2021-03-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | Research in Plant Disease |
| spelling | doaj.art-be58ee19175d459f8b084abb0cd515992022-12-21T23:34:07ZengHanrimwon Publishing CompanyResearch in Plant Disease1598-22622233-91912021-03-01271172310.5423/RPD.2021.27.1.171708Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili PepperJu-Yeon Yoon0V. S. R. Gangireddygari1In-Sook Cho2Bong-Nam Chung3Byung-Dae Yoon4Seung-Kook Choi5 Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea HD Bio-Corporation, KRIBB-BVC, Daejeon 34141, Korea Division of Research Planning and Coordination, Rural Development Administration, Jeonju 54875, KoreaCucumber mosaic virus (CMV), the most prevalent virus in chili pepper (Capsicum annuum L.), negatively affects chili pepper production in South Korea. In this study, foliar spraying with β-glucans obtained from the mycelial walls of the yeast-like fungus Aureobasidium pullulans inhibited CMV infection of chili pepper if applied before virus inoculation. At three concentrations, β-glucans from A. pullulans significantly ameliorated CMV symptoms in treated chili pepper; the effect was greater in plants treated with 0.01% β-glucans than 0.005% or 0.001% β-glucans. Double antibody sandwich enzyme-linked immunosorbent assay showed that these β-glucans treatments resulted in 1.7- to 10-fold reductions in CMV accumulation in the treated chili pepper. The glucans did not act directly on the virus and did not interfere with virus disassembly or replication. Foliar spraying with 0.01% β-glucans from A. pullulans at 24 hr intervals for 3 days significantly increased plant height, the total number of fruit, and the fresh weight of chili pepper fruit. However, the stem diameter of chili pepper treated with β-glucans did not increase significantly. These results indicate that foliar spraying with β-glucans from A. pullulans acts an antiviral agent against CMV infection and stimulates chili pepper growth.http://www.online-rpd.org/upload/pdf/RPD-2021-27-1-17.pdfantiviral activityβ-glucanschili peppercucumber mosaic virus (cmv)pepper growth |
| spellingShingle | Ju-Yeon Yoon V. S. R. Gangireddygari In-Sook Cho Bong-Nam Chung Byung-Dae Yoon Seung-Kook Choi Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper Research in Plant Disease antiviral activity β-glucans chili pepper cucumber mosaic virus (cmv) pepper growth |
| title | Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper |
| title_full | Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper |
| title_fullStr | Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper |
| title_full_unstemmed | Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper |
| title_short | Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper |
| title_sort | effects of β glucans from aureobasidium pullulans on cucumber mosaic virus infection in chili pepper |
| topic | antiviral activity β-glucans chili pepper cucumber mosaic virus (cmv) pepper growth |
| url | http://www.online-rpd.org/upload/pdf/RPD-2021-27-1-17.pdf |
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