Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus
ABSTRACT: Objectives: The aim of this study was to characterize the floR-carrying plasmids originating from Glaesserella parasuis and Actinobacillus indolicus isolated from pigs with respiratory disease in China. Methods: A total of 125 G. parasuis and 28 A. indolicus strains collected between 2009...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716523001571 |
| _version_ | 1827631010190196736 |
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| author | Yongliang Che Renjie Wu Hongjie Li Longbai Wang Xuemin Wu Qiuyong Chen Rujing Chen Lunjiang Zhou |
| author_facet | Yongliang Che Renjie Wu Hongjie Li Longbai Wang Xuemin Wu Qiuyong Chen Rujing Chen Lunjiang Zhou |
| author_sort | Yongliang Che |
| collection | DOAJ |
| description | ABSTRACT: Objectives: The aim of this study was to characterize the floR-carrying plasmids originating from Glaesserella parasuis and Actinobacillus indolicus isolated from pigs with respiratory disease in China. Methods: A total of 125 G. parasuis and 28 A. indolicus strains collected between 2009 and 2022 were screened for florfenicol resistance. Characterization of floR-positive isolates and plasmids were determined by antimicrobial susceptibility testing, serotyping, multilocus sequence typing (MLST), conjugation and transformation assays, whole-genome sequencing (WGS), and phylogenetic analysis. Results: One A. indolicus and six G. parasuis were identified as positive for floR. The six G. parasuis were assigned to four different serovars, including serovars 6, 7, 9, and unknown. In addition to strain XP11, six floR genes were located on plasmids. The six floR-bearing plasmids could be transformed into Pasteurella multocida and divided into two different types, including ∼5000 bp and ∼6000 bp plasmids. The ∼5000 bp plasmids consisting of rep, lysR, mobB, and floR genes, exhibited high similarity among Pasteurellaceae bacteria. Furthermore, the ∼6000 bp plasmids, consisting of rep, lysR, mobC, mobA/L, and floR genes, showed high similarity between G. parasuis and Actinobacillus Spp. Notably, WGS results showed that the floR modules of the two types of plasmids could be transferred and integrated into the diverse Pasteurellaceae- origined plasmids. Conclusion: This study firstly reported the characterization of floR-carrying plasmids from A. indolicus and a non-virulent serovar of G. parasuis in pigs in China and elucidated the transmission mechanism of the floR resistance gene among the Pasteurellaceae family. |
| first_indexed | 2024-03-09T14:15:02Z |
| format | Article |
| id | doaj.art-be59be10226a49e8b017642aed84202d |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-03-09T14:15:02Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-be59be10226a49e8b017642aed84202d2023-11-29T04:24:27ZengElsevierJournal of Global Antimicrobial Resistance2213-71652023-12-0135163171Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicusYongliang Che0Renjie Wu1Hongjie Li2Longbai Wang3Xuemin Wu4Qiuyong Chen5Rujing Chen6Lunjiang Zhou7Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, China; Corresponding author at: Fujian Academy of Agricultural Sciences Institute of Animal Husbandry and Veterinary Medicine, Pudang Road 104＃, Xindian Town, Jin’an District, Fuzhou City, China.ABSTRACT: Objectives: The aim of this study was to characterize the floR-carrying plasmids originating from Glaesserella parasuis and Actinobacillus indolicus isolated from pigs with respiratory disease in China. Methods: A total of 125 G. parasuis and 28 A. indolicus strains collected between 2009 and 2022 were screened for florfenicol resistance. Characterization of floR-positive isolates and plasmids were determined by antimicrobial susceptibility testing, serotyping, multilocus sequence typing (MLST), conjugation and transformation assays, whole-genome sequencing (WGS), and phylogenetic analysis. Results: One A. indolicus and six G. parasuis were identified as positive for floR. The six G. parasuis were assigned to four different serovars, including serovars 6, 7, 9, and unknown. In addition to strain XP11, six floR genes were located on plasmids. The six floR-bearing plasmids could be transformed into Pasteurella multocida and divided into two different types, including ∼5000 bp and ∼6000 bp plasmids. The ∼5000 bp plasmids consisting of rep, lysR, mobB, and floR genes, exhibited high similarity among Pasteurellaceae bacteria. Furthermore, the ∼6000 bp plasmids, consisting of rep, lysR, mobC, mobA/L, and floR genes, showed high similarity between G. parasuis and Actinobacillus Spp. Notably, WGS results showed that the floR modules of the two types of plasmids could be transferred and integrated into the diverse Pasteurellaceae- origined plasmids. Conclusion: This study firstly reported the characterization of floR-carrying plasmids from A. indolicus and a non-virulent serovar of G. parasuis in pigs in China and elucidated the transmission mechanism of the floR resistance gene among the Pasteurellaceae family.http://www.sciencedirect.com/science/article/pii/S2213716523001571SwineGlaesserella parasuisfloRFlorfenicolSmall plasmidPasteurellaceae family |
| spellingShingle | Yongliang Che Renjie Wu Hongjie Li Longbai Wang Xuemin Wu Qiuyong Chen Rujing Chen Lunjiang Zhou Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus Journal of Global Antimicrobial Resistance Swine Glaesserella parasuis floR Florfenicol Small plasmid Pasteurellaceae family |
| title | Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus |
| title_full | Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus |
| title_fullStr | Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus |
| title_full_unstemmed | Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus |
| title_short | Characterization of the plasmids harbouring the florfenicol resistance gene floR in Glaesserella parasuis and Actinobacillus indolicus |
| title_sort | characterization of the plasmids harbouring the florfenicol resistance gene flor in glaesserella parasuis and actinobacillus indolicus |
| topic | Swine Glaesserella parasuis floR Florfenicol Small plasmid Pasteurellaceae family |
| url | http://www.sciencedirect.com/science/article/pii/S2213716523001571 |
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