Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish
Abstract Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that the...
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Nature Portfolio
2018-10-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-018-33817-w |
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| author | Jorke H. Kamstra Selma Hurem Leonardo Martin Martin Leif C. Lindeman Juliette Legler Deborah Oughton Brit Salbu Dag Anders Brede Jan Ludvig Lyche Peter Aleström |
| author_facet | Jorke H. Kamstra Selma Hurem Leonardo Martin Martin Leif C. Lindeman Juliette Legler Deborah Oughton Brit Salbu Dag Anders Brede Jan Ludvig Lyche Peter Aleström |
| author_sort | Jorke H. Kamstra |
| collection | DOAJ |
| description | Abstract Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that these effects are accompanied by changes of DNA methylation possibly inherited by subsequent generations. We assessed DNA methylation in F1 embryos (5.5 hours post fertilization) with whole genome bisulfite sequencing following parental exposure to 8.7 mGy/h for 27 days and found 5658 differentially methylated regions (DMRs). DMRs were predominantly located at known regulatory regions, such as gene promoters and enhancers. Pathway analysis indicated the involvement of DMRs related to similar pathways found with gene expression analysis, such as development, apoptosis and cancers, which could be linked to previous observed developmental defects and genomic instability in the offspring. Follow up of 19 F1 DMRs in F2 and F3 embryos revealed persistent effects up to the F3 generation at 5 regions. These results indicate that ionizing radiation related effects in offspring can be linked to DNA methylation changes that partly can persist over generations. Monitoring DNA methylation could serve as a biomarker to provide an indication of ancestral exposures to ionizing radiation. |
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| id | doaj.art-be5bf4d2934f42899d7fd9c93edd5642 |
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| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:30:11Z |
| publishDate | 2018-10-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-be5bf4d2934f42899d7fd9c93edd56422022-12-21T18:01:44ZengNature PortfolioScientific Reports2045-23222018-10-018111310.1038/s41598-018-33817-wIonizing radiation induces transgenerational effects of DNA methylation in zebrafishJorke H. Kamstra0Selma Hurem1Leonardo Martin Martin2Leif C. Lindeman3Juliette Legler4Deborah Oughton5Brit Salbu6Dag Anders Brede7Jan Ludvig Lyche8Peter Aleström9Faculty of Veterinary Medicine, Norwegian University of Life SciencesFaculty of Veterinary Medicine, Norwegian University of Life SciencesFaculty of Veterinary Medicine, Norwegian University of Life SciencesFaculty of Veterinary Medicine, Norwegian University of Life SciencesInstitute for Environment, Health and Societies, College of Health and Life Sciences, Brunel University LondonFaculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life SciencesFaculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life SciencesFaculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life SciencesFaculty of Veterinary Medicine, Norwegian University of Life SciencesFaculty of Veterinary Medicine, Norwegian University of Life SciencesAbstract Ionizing radiation is known to cause DNA damage, yet the mechanisms underlying potential transgenerational effects of exposure have been scarcely studied. Previously, we observed effects in offspring of zebrafish exposed to gamma radiation during gametogenesis. Here, we hypothesize that these effects are accompanied by changes of DNA methylation possibly inherited by subsequent generations. We assessed DNA methylation in F1 embryos (5.5 hours post fertilization) with whole genome bisulfite sequencing following parental exposure to 8.7 mGy/h for 27 days and found 5658 differentially methylated regions (DMRs). DMRs were predominantly located at known regulatory regions, such as gene promoters and enhancers. Pathway analysis indicated the involvement of DMRs related to similar pathways found with gene expression analysis, such as development, apoptosis and cancers, which could be linked to previous observed developmental defects and genomic instability in the offspring. Follow up of 19 F1 DMRs in F2 and F3 embryos revealed persistent effects up to the F3 generation at 5 regions. These results indicate that ionizing radiation related effects in offspring can be linked to DNA methylation changes that partly can persist over generations. Monitoring DNA methylation could serve as a biomarker to provide an indication of ancestral exposures to ionizing radiation.https://doi.org/10.1038/s41598-018-33817-wTransgenerational EffectsDifferentially Methylated Regions (DMRs)Whole-genome Bisulfite Sequencing (WGBS)WGBS DataDifferentially Expressed Genes (DEGs) |
| spellingShingle | Jorke H. Kamstra Selma Hurem Leonardo Martin Martin Leif C. Lindeman Juliette Legler Deborah Oughton Brit Salbu Dag Anders Brede Jan Ludvig Lyche Peter Aleström Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish Scientific Reports Transgenerational Effects Differentially Methylated Regions (DMRs) Whole-genome Bisulfite Sequencing (WGBS) WGBS Data Differentially Expressed Genes (DEGs) |
| title | Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish |
| title_full | Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish |
| title_fullStr | Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish |
| title_full_unstemmed | Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish |
| title_short | Ionizing radiation induces transgenerational effects of DNA methylation in zebrafish |
| title_sort | ionizing radiation induces transgenerational effects of dna methylation in zebrafish |
| topic | Transgenerational Effects Differentially Methylated Regions (DMRs) Whole-genome Bisulfite Sequencing (WGBS) WGBS Data Differentially Expressed Genes (DEGs) |
| url | https://doi.org/10.1038/s41598-018-33817-w |
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