Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae
One of the obstacles to eradicating <i>paratuberculosis</i> or Johne’s Disease (JD) seems to be the persistence of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (<i>Map</i>) in the environment due to its ability to survive alone or vectoriz...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/11/1781 |
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| author | Vincent Rochard Thierry Cochard Stéphanie Crapart Vincent Delafont Jean-Louis Moyen Yann Héchard Franck Biet |
| author_facet | Vincent Rochard Thierry Cochard Stéphanie Crapart Vincent Delafont Jean-Louis Moyen Yann Héchard Franck Biet |
| author_sort | Vincent Rochard |
| collection | DOAJ |
| description | One of the obstacles to eradicating <i>paratuberculosis</i> or Johne’s Disease (JD) seems to be the persistence of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (<i>Map</i>) in the environment due to its ability to survive alone or vectorized. It has been shown that <i>Map</i> is widely distributed in soils and water. Previously, we isolated amoebae associated with <i>Map</i> strains in the environment of bovines from an infected herd. This work aims to verify our working hypothesis, which suggests that amoebae may play a role in the transmission of JD. In this study, we sampled water in the vicinity of herds infected with <i>Map</i> or <i>Mycobacterium bovis</i> (<i>M. bovis</i>) and searched for amoebae and mycobacteria. Live amoebae were recovered from all samples. Among these amoebae, four isolates associated with the presence of mycobacteria were identified and characterized. <i>Map</i> and other mycobacterial species were detected by qPCR and, in some cases, by culture. This study suggests that amoebae and <i>Map</i> may be found in the same environment and might represent a risk of exposure of animals to pathogenic mycobacteria. These data open up new perspectives on the control measures to be put in place to prevent contamination by <i>Map</i>. |
| first_indexed | 2024-03-11T03:12:49Z |
| format | Article |
| id | doaj.art-be5dbbeed9964826bdf02079390f83e0 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T03:12:49Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-be5dbbeed9964826bdf02079390f83e02023-11-18T07:29:37ZengMDPI AGAnimals2076-26152023-05-011311178110.3390/ani13111781Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental AmoebaeVincent Rochard0Thierry Cochard1Stéphanie Crapart2Vincent Delafont3Jean-Louis Moyen4Yann Héchard5Franck Biet6Laboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l’Eau, F-86073 Poitiers, FranceInstitut National de Recherche pour l’Agriculture—INRAE, Université de Tours, ISP, F-37390 Nouzilly, FranceLaboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l’Eau, F-86073 Poitiers, FranceLaboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l’Eau, F-86073 Poitiers, FranceLaboratoire Départemental d’Analyse et de Recherche de la Dordogne, F-24660 Coulounieix-Chamiers, FranceLaboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l’Eau, F-86073 Poitiers, FranceInstitut National de Recherche pour l’Agriculture—INRAE, Université de Tours, ISP, F-37390 Nouzilly, FranceOne of the obstacles to eradicating <i>paratuberculosis</i> or Johne’s Disease (JD) seems to be the persistence of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (<i>Map</i>) in the environment due to its ability to survive alone or vectorized. It has been shown that <i>Map</i> is widely distributed in soils and water. Previously, we isolated amoebae associated with <i>Map</i> strains in the environment of bovines from an infected herd. This work aims to verify our working hypothesis, which suggests that amoebae may play a role in the transmission of JD. In this study, we sampled water in the vicinity of herds infected with <i>Map</i> or <i>Mycobacterium bovis</i> (<i>M. bovis</i>) and searched for amoebae and mycobacteria. Live amoebae were recovered from all samples. Among these amoebae, four isolates associated with the presence of mycobacteria were identified and characterized. <i>Map</i> and other mycobacterial species were detected by qPCR and, in some cases, by culture. This study suggests that amoebae and <i>Map</i> may be found in the same environment and might represent a risk of exposure of animals to pathogenic mycobacteria. These data open up new perspectives on the control measures to be put in place to prevent contamination by <i>Map</i>.https://www.mdpi.com/2076-2615/13/11/1781amoebaeenvironmentJohne’s disease<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i>transmission |
| spellingShingle | Vincent Rochard Thierry Cochard Stéphanie Crapart Vincent Delafont Jean-Louis Moyen Yann Héchard Franck Biet Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae Animals amoebae environment Johne’s disease <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> transmission |
| title | Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae |
| title_full | Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae |
| title_fullStr | Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae |
| title_full_unstemmed | Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae |
| title_short | Presence of Non-Tuberculous Mycobacteria Including <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> Associated with Environmental Amoebae |
| title_sort | presence of non tuberculous mycobacteria including i mycobacterium avium i subsp i paratuberculosis i associated with environmental amoebae |
| topic | amoebae environment Johne’s disease <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> transmission |
| url | https://www.mdpi.com/2076-2615/13/11/1781 |
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