Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)
Identification of filamentous fungi by conventional phenotypic methods are time-consuming, and a correct identification at the species level is prone to errors. Therefore, a more accurate and faster time-to-results, and cost-effective technique, is required, such as the Matrix-Assisted Laser Desorpt...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1362 |
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| author | Juan C. Gómez-Velásquez Natalia Loaiza-Díaz Gilma Norela Hernández Nelson Lima Ana C. Mesa-Arango |
| author_facet | Juan C. Gómez-Velásquez Natalia Loaiza-Díaz Gilma Norela Hernández Nelson Lima Ana C. Mesa-Arango |
| author_sort | Juan C. Gómez-Velásquez |
| collection | DOAJ |
| description | Identification of filamentous fungi by conventional phenotypic methods are time-consuming, and a correct identification at the species level is prone to errors. Therefore, a more accurate and faster time-to-results, and cost-effective technique, is required, such as the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). In this study, we describe the development of an in-house spectra library for the identification of filamentous fungi frequently isolated from patients with infections. An in-house spectra library was constructed using 14 reference strains grown in solid medium. Clinical isolates were identified either by the in-house spectra library or the Biotyper commercial library from Bruker Daltonics. Fungal identification was carried following the Biotyper’s established scores: ≤1.699: not reliably identified (NRI); 1.700–1.999: genus-level; ≥2.000: species-level. Clinical isolates were identified, with the in-house library, at species- and genus-level at 88.70% (55) and 3.22% (2), respectively. While 4.80% (3) was NRI and 3.22% (2) was discrepant concerning sequencing. On the contrary, identification up to species and genus-level with the commercial library was 44.44% (16) and 22.22% (8), respectively. NRI and the discrepancy was 30.55% (11) and 2.77% (1), respectively. For the reaming 26 isolates, 16 from <i>Neoscytalidium dimidiatum</i> and 10 from <i>Sporothrix</i> spp., respectively, the absence of spectrum and the specific spectra within the <i>Sporothrix</i> complex in the commercial library resulted in the inability to obtain an identification. In conclusion, the current results advocate the importance that each clinical microbiological laboratory needs to develop an ad hoc library associated with the MALDI-TOF MS fungal identification to overcome the limitations of the available commercial libraries. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T16:32:11Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj.art-be6132ed7bc04f0586db90216ce6e2532023-11-20T12:46:15ZengMDPI AGMicroorganisms2076-26072020-09-0189136210.3390/microorganisms8091362Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)Juan C. Gómez-Velásquez0Natalia Loaiza-Díaz1Gilma Norela Hernández2Nelson Lima3Ana C. Mesa-Arango4Laboratorio Clínico Grupo Synlab, Calle 19 A, Medellín 050021, ColombiaLaboratorio Clínico Grupo Synlab, Calle 19 A, Medellín 050021, ColombiaGrupo Académico de Epidemiología Clínica, Facultad de Medicina, Universidad de Antioquia, Carrera 51 D, Medellín 050010, ColombiaCEB-Centre of Biological Engineering, Micoteca da Universidade do Minho (MUM), University of Minho, Campus de Gualtar, 4710-057 Braga, PortugalGrupo de Investigación Dermatológica, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia, Avenida Juan del Corral, Medellín 050010, ColombiaIdentification of filamentous fungi by conventional phenotypic methods are time-consuming, and a correct identification at the species level is prone to errors. Therefore, a more accurate and faster time-to-results, and cost-effective technique, is required, such as the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). In this study, we describe the development of an in-house spectra library for the identification of filamentous fungi frequently isolated from patients with infections. An in-house spectra library was constructed using 14 reference strains grown in solid medium. Clinical isolates were identified either by the in-house spectra library or the Biotyper commercial library from Bruker Daltonics. Fungal identification was carried following the Biotyper’s established scores: ≤1.699: not reliably identified (NRI); 1.700–1.999: genus-level; ≥2.000: species-level. Clinical isolates were identified, with the in-house library, at species- and genus-level at 88.70% (55) and 3.22% (2), respectively. While 4.80% (3) was NRI and 3.22% (2) was discrepant concerning sequencing. On the contrary, identification up to species and genus-level with the commercial library was 44.44% (16) and 22.22% (8), respectively. NRI and the discrepancy was 30.55% (11) and 2.77% (1), respectively. For the reaming 26 isolates, 16 from <i>Neoscytalidium dimidiatum</i> and 10 from <i>Sporothrix</i> spp., respectively, the absence of spectrum and the specific spectra within the <i>Sporothrix</i> complex in the commercial library resulted in the inability to obtain an identification. In conclusion, the current results advocate the importance that each clinical microbiological laboratory needs to develop an ad hoc library associated with the MALDI-TOF MS fungal identification to overcome the limitations of the available commercial libraries.https://www.mdpi.com/2076-2607/8/9/1362in-house libraryfilamentous fungiMALDI-TOF MSclinical isolates |
| spellingShingle | Juan C. Gómez-Velásquez Natalia Loaiza-Díaz Gilma Norela Hernández Nelson Lima Ana C. Mesa-Arango Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia) Microorganisms in-house library filamentous fungi MALDI-TOF MS clinical isolates |
| title | Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia) |
| title_full | Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia) |
| title_fullStr | Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia) |
| title_full_unstemmed | Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia) |
| title_short | Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia) |
| title_sort | development and validation of an in house library for filamentous fungi identification by maldi tof ms in a clinical laboratory in medellin colombia |
| topic | in-house library filamentous fungi MALDI-TOF MS clinical isolates |
| url | https://www.mdpi.com/2076-2607/8/9/1362 |
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