Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats
The present study is aimed to elucidate the possible involvement of the thermosensitive TRPM2 ion channel in changing of the temperature sensitivity of the hypothalamus after different cold exposures—long-term adaptation to cold and short-term cooling. Quantitative RT-PCR was used to study the expre...
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MDPI AG
2023-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/2/65 |
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| author | Anna A. Evtushenko Irina P. Voronova Tamara V. Kozyreva |
| author_facet | Anna A. Evtushenko Irina P. Voronova Tamara V. Kozyreva |
| author_sort | Anna A. Evtushenko |
| collection | DOAJ |
| description | The present study is aimed to elucidate the possible involvement of the thermosensitive TRPM2 ion channel in changing of the temperature sensitivity of the hypothalamus after different cold exposures—long-term adaptation to cold and short-term cooling. Quantitative RT-PCR was used to study the expression of the gene of thermosensitive TRPM2 ion channel in the hypothalamus in the groups of control (kept for 5 weeks at +20 to +22 °C) and cold-adapted (5 weeks at +4 to +6 °C) rats, as well as in the groups of animals which were subjected to acute cooling (rapid or slow) with subsequent restoration of body temperature to the initial level. It has been shown that after long-term adaptation to cold, the decrease in the <i>Trpm2</i> gene expression was observed in the hypothalamus, while a short-term cooling does not affect the expression of the gene of this ion channel. Thus, long-term adaptation to cold results in the decrease in the activity not only of the TRPV3 ion channel gene, as shown earlier, but also of the <i>Trpm2</i> gene in the hypothalamus. The overlapping temperature ranges of the functioning of these ion channels and their unidirectional changes during the adaptation of the homoeothermic organism to cold suggest their functional interaction. The decrease in the <i>Trpm2</i> gene expression may indicate the participation of this ion channel in adaptive changes in hypothalamic thermosensitivity, but only as a result of long-term cold exposure and not of a short-term cooling. These processes occurring at the genomic level are one of the molecular mechanisms of the adaptive changes. |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T08:59:17Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-be706b4293d44cc1a8b6bad24c581b782023-11-16T19:50:37ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-014521002101110.3390/cimb45020065Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of RatsAnna A. Evtushenko0Irina P. Voronova1Tamara V. Kozyreva2Department of Thermophysiology, Scientific Research Institute of Neurosciences and Medicine, 630117 Novosibirsk, RussiaDepartment of Thermophysiology, Scientific Research Institute of Neurosciences and Medicine, 630117 Novosibirsk, RussiaDepartment of Thermophysiology, Scientific Research Institute of Neurosciences and Medicine, 630117 Novosibirsk, RussiaThe present study is aimed to elucidate the possible involvement of the thermosensitive TRPM2 ion channel in changing of the temperature sensitivity of the hypothalamus after different cold exposures—long-term adaptation to cold and short-term cooling. Quantitative RT-PCR was used to study the expression of the gene of thermosensitive TRPM2 ion channel in the hypothalamus in the groups of control (kept for 5 weeks at +20 to +22 °C) and cold-adapted (5 weeks at +4 to +6 °C) rats, as well as in the groups of animals which were subjected to acute cooling (rapid or slow) with subsequent restoration of body temperature to the initial level. It has been shown that after long-term adaptation to cold, the decrease in the <i>Trpm2</i> gene expression was observed in the hypothalamus, while a short-term cooling does not affect the expression of the gene of this ion channel. Thus, long-term adaptation to cold results in the decrease in the activity not only of the TRPV3 ion channel gene, as shown earlier, but also of the <i>Trpm2</i> gene in the hypothalamus. The overlapping temperature ranges of the functioning of these ion channels and their unidirectional changes during the adaptation of the homoeothermic organism to cold suggest their functional interaction. The decrease in the <i>Trpm2</i> gene expression may indicate the participation of this ion channel in adaptive changes in hypothalamic thermosensitivity, but only as a result of long-term cold exposure and not of a short-term cooling. These processes occurring at the genomic level are one of the molecular mechanisms of the adaptive changes.https://www.mdpi.com/1467-3045/45/2/65TRP ion channelsTRPM2gene expressionhypothalamuslong-term adaptation to coldshort-term cooling |
| spellingShingle | Anna A. Evtushenko Irina P. Voronova Tamara V. Kozyreva Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats Current Issues in Molecular Biology TRP ion channels TRPM2 gene expression hypothalamus long-term adaptation to cold short-term cooling |
| title | Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats |
| title_full | Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats |
| title_fullStr | Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats |
| title_full_unstemmed | Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats |
| title_short | Effect of Long-Term Adaptation to Cold and Short-Term Cooling on the Expression of the TRPM2 Ion Channel Gene in the Hypothalamus of Rats |
| title_sort | effect of long term adaptation to cold and short term cooling on the expression of the trpm2 ion channel gene in the hypothalamus of rats |
| topic | TRP ion channels TRPM2 gene expression hypothalamus long-term adaptation to cold short-term cooling |
| url | https://www.mdpi.com/1467-3045/45/2/65 |
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